F4018

Anti-Mouse IgG (Fab specific)−FITC antibody produced in goat

affinity isolated antibody, buffered aqueous solution

• Synonym(e): Goat Anti-Mouse IgG (Fab specific)−Fluorescein isothiocyanate
• UNSPSC Code: 12352203
• NACRES: NA.46
• MDL number: MFCD00162622
• Conjugate: FITC conjugate
• Clone: polyclonal
• Application: IF, IHC (p)
• Citations: 21

Eigenschaften

• biological source: goat
• Quality Segment: 200
• conjugate: FITC conjugate
• antibody form: affinity isolated antibody
• antibody product type: secondary antibodies
• clone: polyclonal
• form: buffered aqueous solution
• storage condition: protect from light
• technique(s): immunohistochemistry (formalin-fixed, paraffin-embedded sections): 1:200, indirect immunofluorescence: 1:64
• storage temp.: −20°C
• target post-translational modification: unmodified

Beschreibung

General description

IgG (immunoglobulin G) is found in four subclasses IgG1, IgG2, IgG3 and IgG4. IgG has a monomeric structure.

IgG antibody subtype is the most abundant serum immunoglobulins of the immune system. It is secreted by B cells and is found in blood and extracellular fluids and provides protection from infections caused by bacteria, fungi and viruses. Maternal IgG is transferred to fetus through the placenta that is vital for immune defence of the neonate against infections.

Immunogen

Purified mouse IgG Fab fragment

Application

Anti-Mouse IgG (Fab specific) FITC antibody produced in goat has been used in :

• fluorescent staining
• immunocytochemistry
• immunofuorescence

Biochem/physiol Actions

Anti-Mouse IgG (Fab specific)-FITC antibody is specific for Fab fragment of all mouse IgG subclasses. Goat anti-Mouse IgG is conjugated to FITC and then purified by gel filtration to remove free FITC. The antibody is adsorbed with human IgG to ensure minimal cross reactivity. The conjugate shows no reactivity with mouse IgG, Fc fragment, human, bovine, or horse serum.

IgG (immunoglobulin G) antibody provides protection from infections caused by bacteria, fungi and viruses. Maternal IgG is transferred to fetus through the placenta that is vital for immune defence of the neonate against infections. IgG antibody participates in complement fixation and opsonization.

Physical form

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.

Preparation Note

Adsorbed to reduce background staining with bovine, horse, or human samples.

Other Notes

Antibody adsorbed with bovine, equine and human serum proteins.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Sicherheitshinweise

• Lagerklasse: 10 - Combustible liquids
• wgk: nwg
• flash_point_f: Not applicable
• flash_point_c: Not applicable
• ppe: Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)