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492016

NP-40-Alternative

Synonym(e):

NP-40-Alternative – CAS 9016-45-9 – Calbiochem, Nonylphenylpolyethylenglykol

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PackungsgrößeSKUVerfügbarkeitPreis
100 mL

Voraussichtlich HEUTE zu versendenvonStrasbourg

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500 mL

Voraussichtlich HEUTE zu versendenvonStrasbourg

331,00 €
1000 mL

Voraussichtlich HEUTE zu versendenvonStrasbourg

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Über diesen Artikel

CAS-Nummer:
UNSPSC Code:
12161902
NACRES:
NA.25
MDL number:

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description

RTECS - MD0905000

Quality Segment

form

liquid

manufacturer/tradename

Calbiochem®

storage condition

OK to freeze, desiccated

color

clear colorless

CMC

50 - 300 μM

solubility

water: 25 mg/mL, aqueous buffer: soluble

density

1.06 g/mL

application(s)

(Isolating membrane proteins)

shipped in

ambient

storage temp.

15-25°C

SMILES string

O(CCOc1ccc(cc1)CCCCCCCCC)CCO

InChI

1S/C19H32O3/c1-2-3-4-5-6-7-8-9-18-10-12-19(13-11-18)22-17-16-21-15-14-20/h10-13,20H,2-9,14-17H2,1H3

InChI key

BLXVTZPGEOGTGG-UHFFFAOYSA-N

General description

Ein nicht-ionisches Tensid, das bei der Isolierung und Reinigung von funktionellen Membranproteinkomplexen und bei der zweidimensionalen (2D-)Gelelektrophorese verwendet wird. Ähnliche Anwendung wie bei NP-40 und TRITON X-100 Detergens (Katalog-Nr. <;A href="https://./?[islink]ViewProductDetail-BySKU&;#124;&;#124;SKU&;#124;EMD_BIO-648462&;#124;[/islink]">;648462<;/A>;).

Application

NP-40 Alternative has been used:
  • as a component of the lysis buffer for solubilizing proteins from transfected HEK293 cells.
  • as a component of the wash buffer for efficient solubilization of biotinylated plasma proteins in plasma protein profiling and chromatin immunoprecipitation (ChIP).
  • for the purification of doublet microtubules from cilia for single particle cryo-electron microscopy (cryo-EM).

Other Notes

Ripoche, P., et al. 1982. Biochim. Biophys. Acta693, 497.

Legal Information

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany
Triton is a trademark of The Dow Chemical Company or an affiliated company of Dow

Disclaimer

Toxizität: Reizstoff (B)

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Dieser Artikel
49201856741X45
form

liquid

form

solution

form

liquid

form

-

application(s)

(Isolating membrane proteins)

application(s)

(Isolating membrane proteins)

application(s)

-

application(s)

-

storage temp.

15-25°C

storage temp.

2-8°C

storage temp.

-

storage temp.

-

density

1.06 g/mL

density

-

density

1.06 g/mL at 20 °C

density

-

CMC

50 - 300 μM

CMC

50 - 300 μM

CMC

0.08 mM (20-25°C)

CMC

-

solubility

water: 25 mg/mL, aqueous buffer: soluble

solubility

water: soluble

solubility

-

solubility

-


pictograms

Exclamation markEnvironment

Lagerklasse

11 - Combustible Solids

flash_point_f

503.6 °F - Pensky-Martens closed cup - (own results)

flash_point_c

262 °C - Pensky-Martens closed cup - (own results)

signalword

Danger

Hazard Classifications

Aquatic Acute 1 - Aquatic Chronic 1 - ED ENV 1 - Eye Irrit. 2 - Skin Irrit. 2


Zulassungslistungen

Zulassungslistungen werden hauptsächlich für chemische Produkte erstellt. Für nicht-chemische Produkte können hier nur begrenzte Angaben gemacht werden. Kein Eintrag bedeutet, dass keine der Komponenten gelistet ist. Es liegt in der Verantwortung des Benutzers, die sichere und legale Verwendung des Produkts zu gewährleisten.

9016-45-9

CAS No.

9016-45-9

CAS No.

9016-45-9

CAS No.


Analysenzertifikate (COA)

Suchen Sie nach Analysenzertifikate (COA), indem Sie die Lot-/Chargennummer des Produkts eingeben. Lot- und Chargennummern sind auf dem Produktetikett hinter den Wörtern ‘Lot’ oder ‘Batch’ (Lot oder Charge) zu finden.

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Ju Gao et al.
Autophagy, 12(4), 632-647 (2016-04-07)
During proteasomal stress, cells can alleviate the accumulation of polyubiquitinated proteins by targeting them to perinuclear aggresomes for autophagic degradation, but the mechanism underlying the activation of this compensatory pathway remains unclear. Here we report that PINK1-s, a short form
Gustav Roder et al.
European journal of immunology, 39(10), 2682-2694 (2009-09-04)
Tapasin (Tpn) is an ER chaperone that is uniquely dedicated to MHC-I biosynthesis. It binds MHC-I molecules, integrates them into peptide-loading complexes, and exerts quality control of the bound peptides; only when an "optimal peptide" is bound will the MHC-I
Ying Zhang et al.
Cancer research, 79(19), 4994-5007 (2019-08-09)
Human astrocytomas and oligodendrogliomas are defined by mutations of the metabolic enzymes isocitrate dehydrogenase (IDH) 1 or 2, resulting in the production of the abnormal metabolite D-2 hydroxyglutarate. Here, we studied the effect of mutant IDH on cell proliferation and



Global Trade Item Number

SKUGTIN
492016-100ML07790788050832
492016-1000ML04055977273489
492016-500ML04055977273496

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  1. How can I determine the shelf life / expiration / retest date of this product?

    1 Antwort
    1. If this product has an expiration or retest date, it will be shown on the Certificate of Analysis (COA, CofA). If there is no retest or expiration date listed on the product's COA, we do not have suitable stability data to determine a shelf life. For these products, the only date on the COA will be the release date; a retest, expiration, or use-by-date will not be displayed.
      For all products, we recommend handling per defined conditions as printed in our product literature and website product descriptions. We recommend that products should be routinely inspected by customers to ensure they perform as expected.
      For products without retest or expiration dates, our standard warranty of 1 year from the date of shipment is applicable.
      For more information, please refer to the Product Dating Information document: https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/marketing/global/documents/418/501/product-dating-information-06-25-mk.pdf

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  2. How is shipping temperature determined? And how is it related to the product storage temperature?

    1 Antwort
    1. Products may be shipped at a different temperature than the recommended long-term storage temperature. If the product quality is sensitive to short-term exposure to conditions other than the recommended long-term storage, it will be shipped on wet or dry-ice. If the product quality is NOT affected by short-term exposure to conditions other than the recommended long-term storage, it will be shipped at ambient temperature. As shipping routes are configured for minimum transit times, shipping at ambient temperature helps control shipping costs for our customers. For more information, please refer to the Storage and Transport Conditions document: https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/marketing/global/documents/316/622/storage-transport-conditions-mk.pdf

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