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M4530

Medium 199

With Earle′s salts, ʟ-glutamine and sodium bicarbonate, liquid, sterile-filtered, suitable for cell culture

Medium 199
1 of 1 reviewers took part in a promotion

Synonyme(s) :

M199 Medium, TCM 199

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ConditionnementRéférenceDisponibilitéPrix
100 mL
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19,70 $
500 mL
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44,60 $
1 L
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92,30 $
6 x 500 mL
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246,00 $
6 x 1 L
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441,00 $

A propos de cet article

UNSPSC Code:
12352207
NACRES:
NA.75

19,70 $


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Nom du produit

Medium 199, With Earle′s salts, L-glutamine and sodium bicarbonate, liquid, sterile-filtered, suitable for cell culture

Quality Segment

sterility

sterile-filtered

form

liquid

technique(s)

cell culture | mammalian: suitable

impurities

endotoxin, tested

components

NaHCO3: yes
L-glutamine: yes
HEPES: no
Earle’s salts (5% CO2): yes
phenol red: yes
sodium pyruvate: no

shipped in

ambient

storage temp.

2-8°C

General description

Complete liquid version of the original M 199 formulation published by Morgan.
Medium 199 is a combination of vitamins, amino acids, and other factors that were originally developed as a completely defined media formulation for the culture of primary explants. This medium, when properly supplemented has broad applicability, particularly for non-transformed cells. Medium 199 is widely used for vaccine production, in vitro cultivation of primary pancreatic explants, and lens tissues.

Application

Medium 199 has been used:
  • as a suspension media to maintain the primary neuronal cultures
  • as a component of the collagen gel for ex vivo embryonic heart culture
  • to maintain the ovarian tissue sections as part of the slow-freezing protocol


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1 of 1

Cet article
M2154M2520M3769
form

liquid

form

liquid

form

powder

form

powder

sterility

sterile-filtered

sterility

sterile-filtered

sterility

-

sterility

-

technique(s)

cell culture | mammalian: suitable

technique(s)

cell culture | mammalian: suitable

technique(s)

cell culture | mammalian: suitable

technique(s)

cell culture | mammalian: suitable

components

NaHCO3: yes
L-glutamine: yes
HEPES: no
Earle’s salts (5% CO2): yes
phenol red: yes
sodium pyruvate: no

components

sodium pyruvate: no
L-glutamine: no
NaHCO3: yes
HEPES: no
phenol red: yes
Earle’s salts (5% CO2): yes

components

L-glutamine: yes
phenol red: yes
Earle’s salts (5% CO2): yes
NaHCO3: no
sodium pyruvate: no
HEPES: 25 mM

components

L-glutamine: no
phenol red: no
HEPES: no
sodium pyruvate: no
Earle’s salts (5% CO2): yes
NaHCO3: no

shipped in

ambient

shipped in

ambient

shipped in

ambient

shipped in

ambient

storage temp.

2-8°C

storage temp.

2-8°C

storage temp.

2-8°C

storage temp.

2-8°C


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Classe de stockage

10 - Combustible liquids

wgk

WGK 1

flash_point_f

Not applicable

flash_point_c

Not applicable



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Articles

Calcium is an ionically stable divalent cation with important beneficial and toxic properties in cell culture. It is a component of a wide range of cell culture media.

Medium 199 supports non-transformed cell cultivation in virology and vaccine production, offering broad species applicability.

Contenu apparenté

Discover the formulation for Medium 199 for cell culture applications, including vaccine development.


Li Ma et al.
The journals of gerontology. Series A, Biological sciences and medical sciences, 72(4), 489-498 (2016-08-28)
We previously reported that pretreatment with the potent antioxidant TEMPOL improves mitochondrial function and restores preconditioning in the aging heart. Because mitophagy is implicated in cardiac preconditioning and declines with age, this study was designed to investigate how age influences
Patrick C Baer et al.
International journal of molecular sciences, 19(6) (2018-06-13)
Stem cell-based therapies require cells with a maximum regenerative capacity in order to support regeneration after tissue injury and organ failure. Optimization of this regenerative potential of mesenchymal stromal/stem cells (MSC) or their conditioned medium by in vitro preconditioning regimens
Vladimir Jovasevic et al.
Journal of virology, 82(7), 3311-3319 (2008-01-25)
In this report we propose a model in which after the herpes simplex virus (HSV) capsid docks at the nuclear pore, the tegument protein attached to the capsid must be cleaved by a serine or a cysteine protease in order



Numéro d'article de commerce international

RéférenceGTIN
M4530-100ML04061837847363
M4530-500ML04061837847370
M4530-1L04061834059028
M4530-6X1L04061834059035
M4530-6X500ML04061834059042

Questions

Reviews

1 of 1 reviewers took part in a promotion

Active Filters

  1. London, U.K.
    • Reviews 3
    • Votes 6
    5 out of 5 stars.

    Good value for money and can be used as the basal medium for endothelial cell growth

    Really good value for money and always arrives with a good shelf-life in terms of expiry date. Great for adding cytokines + hydrocortisone to to create your own endothelial cell growth medium - has kept my HUVECs consistently happy since I started using it in 2018. Also great to use when isolating human primary endothelial cells.

    Helpful?