LIG2
QuickLink™ DNA Ligation Kit
joins blunt-end and sticky-end DNA fragments
Synonyme(s) :
DNA Ligation Kit, Ligation Kit
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| Conditionnement | Référence | Disponibilité | Prix |
|---|---|---|---|
| 1 kit | Veuillez contacter notre Service Clients pour connaître la disponibilité de ce produit. | 694,00 $ |
A propos de cet article
UNSPSC Code:
12352200
EC Number:
232-770-0
NACRES:
NA.55
694,00 $
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Molecular Biology
Quality Level
form
liquid
usage
kit sufficient for 50 ligation reactions
technique(s)
molecular cloning: suitable
shipped in
dry ice
storage temp.
−70°C
General description
Sigma′s DNA Ligation Kit contains all of the reagents necessary to perform DNA ligation reactions at room temperature using blunt or sticky ends. This kit replaces previous workflows requiring cooking steps and long incubations. Pre-made buffers allow for fast & easy setup.
Application
QuickLink™ DNA Ligation Kit has been used for the ligation of pGL2-basic luciferase-reporter plasmid with vascular endothelial growth factor (VEGF) promoter sequence and for the ligation of NLR family caspase activating and recruitment domain (CARD)-containing protein 4 (Nlrc4) promoter with the luciferase plasmid vector pGL4.10-luc2
The QuickLink™ DNA Ligation Kit is suitable for:
- Joining blunt or cohesive-end fragments of DNA into a cloning vector
- Recircularization of linear DNA
- Formation of concatamers
- dsDNA nick repair
Biochem/physiol Actions
One of the most important steps in the cloning process is the ligation of linear DNA into a cloning vector. DNA ligations are performed by incubating DNA fragments with appropriately linearized cloning vectors in the presence of buffer, ATP, and DNA ligase. Many parameters affect ligations such as the relative ratio of insert to vector, the quality and type of the DNA ends, the temperature of ligation and the concentration of DNA.
Features and Benefits
- Fast 5 minutes ligation
- High ligation efficiency
- Room temperature reactions – no cooling required
- Perform bacterial transformation with the reaction mixture
Other Notes
Sufficient for 50 reactions:
- 500uL 2X Ligation Buffer A (L9537)
- 100uL 5X Ligation Buffer B (L9662)
- 250 units T4 DNA Ligase (D2886) in 50% glycerol with 10 mM Tris-HCl (pH 7.5) 50 mM KCl, and 1 mM DTT
Legal Information
QuickLink is a trademark of Sigma-Aldrich Co. LLC
1 of 1
Cet article | |||
|---|---|---|---|
| technique(s) molecular cloning: suitable | technique(s) - | technique(s) DNA purification: suitable | technique(s) - |
| usage kit sufficient for 50 ligation reactions | usage 1 kit sufficient for 150 ligation reactions | usage sufficient for 350 purifications | usage sufficient for 40 reactions (DNA ligation) |
| grade Molecular Biology | grade Molecular Biology | grade - | grade - |
| storage temp. −70°C | storage temp. −20°C | storage temp. 15-25°C | storage temp. −20°C |
| shipped in dry ice | shipped in wet ice | shipped in - | shipped in - |
| form liquid | form - | form - | form - |
Classe de stockage
10 - Combustible liquids
flash_point_f
Not applicable
flash_point_c
Not applicable
signalword
Danger
hcodes
pcodes
Hazard Classifications
Resp. Sens. 1
wgk
WGK 1
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Protocoles
T4 DNA ligase is used for the joining of DNA molecules with compatible cohesive (sticky) termini, joining of blunt ended double stranded DNA molecules
Contenu apparenté
I R Lehman
Science (New York, N.Y.), 186(4166), 790-797 (1974-11-29)
DNA ligase of E. coli is a polypeptide of molecular weight 75,000. The comparable T4-induced enzyme is somewhat smaller (63,000 to 68,000). Both enzymes catalyze the synthesis of phosphodiester bonds between adjacent 5'-phosphoryl and 3'-hydroxyl groups in nicked duplex DNA
Constitutive Activation of the Nlrc4 inflammasome prevents hepatic fibrosis and promotes hepatic regeneration after partial hepatectomy
DeSantis DA, et al.
Mediators of Inflammation, 2015(2), 455-465 (2015)
R Rossi et al.
Nucleic acids research, 25(11), 2106-2113 (1997-06-01)
ATP-dependent DNA ligases are essential enzymes in both DNA replication and DNA repair processes. Here we report a functional characterization of the T4 DNA ligase. One N-terminal and two C-terminal deletion mutants were expressed in Escherichia coli as histidine- tagged
Numéro d'article de commerce international
| Référence | GTIN |
|---|---|
| LIG2-1KT | 04061833967225 |