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D6898

Deoxyribonucleic acid sodium salt from herring testes

Type XIV

Synonyme(s) :

DNA

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ConditionnementRéférenceDisponibilitéPrix
250 mg
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83,40 $
1 g
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581,00 $
10 g
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1 060,00 $

A propos de cet article

Numéro CAS:
UNSPSC Code:
41106305
eCl@ss:
32160414
NACRES:
NA.51
MDL number:
Form:
solid
Solubility:
water: 2 mg/mL, clear to hazy, colorless
Storage temp.:
2-8°C

83,40 $


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type

Type XIV

Quality Segment

form

solid

color

white

solubility

water: 2 mg/mL, clear to hazy, colorless

storage temp.

2-8°C

General description

Deoxyribonucleic acid sodium salt from herring testes, also known as HtDNA is a natural source of DNA designed for DNA binding agent studies. The detection limits of HtDNA are around 5.0ng and linear ranges of light scattering intensity (I(LS)) is 0.05-2.5 mµg.[1]

Application

Deoxyribonucleic acid sodium salt from herring testes is used in studies of DNA binding agents that modulate DNA structure and function. Herring testes DNA is also used in physicochemical studies of DNA behavior in solution.

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1 of 1

Cet article
D3159D1626D3664
Quality Level

200

Quality Level

200

Quality Level

200

Quality Level

200

form

solid

form

powder

form

solid

form

powder or crystals

solubility

water: 2 mg/mL, clear to hazy, colorless

solubility

-

solubility

H2O: 2 mg/mL, clear to hazy

solubility

-

color

white

color

-

color

-

color

-

storage temp.

2-8°C

storage temp.

2-8°C

storage temp.

2-8°C

storage temp.

2-8°C

type

Type XIV

type

-

type

-

type

-


Classe de stockage

13 - Non Combustible Solids

wgk

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, type N95 (US)



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Certificats d'analyse (COA)

Lot/Batch Number

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Numéro d'article de commerce international

RéférenceGTIN
D6898-250MG04061833587966
D6898-1G04061826761694
D6898-10G04061833587959

Questions

1–3 of 3 Questions  
  1. What is the species for product D6898? It's only listed as Herring on CofO.

    1 answer
    1. The species is Clupea harengus.

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  2. What buffer should be used to dissolve this for long term storage? Is there any stock concentration recommendations?

    1 answer
    1. The solubility of this product is tested at 2 mg/mL in H2O. Solutions of DNA have been stored successfully for several months at 4 °C, in 10 mM Tris, pH 7.5 - 8.0, with 1 mM EDTA, without a bacteriostatic agent. At low concentrations (25 μg/ml), DNA tends to absorb onto the surfaces of plastic tubes. It is not recommended to store DNA in alkaline solutions since DNA tends to degrade at a pH greater than 8.0.

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  3. What is the molecular weight range of Product D6898, Deoxyribonucleic acid sodium salt from herring testes, Type XIV? What is the difference between Product D6898 and D3159 (Deoxyribonucleic acid from herring sperm)?

    1 answer
    1. The molecular weight of this product is not determined . However, one publication has described "linear DNA-type XIV from herring testes sodium salt (Na content 6.2%, H2O content 4.5%)" as having a molecular weight between 400 and 1000 base pairs with a center of distribution at 700 bps". Assuming the average molecular weight of a base pair (bp) to be 649.0 g/mol, these three MW values calculate as follows:
      400 bp = 259,600 MW
      700 bp = 454,300 MW
      1000 bp = 649,000 MW
      Wu, J.-C., et al., Neutron and X-ray reflectivity studies on DNA adsorption on mixed DPPC/DC-Cholesterol monolayers. Physica B, 385-386, 841-844 (2006). (August 2007).


      The difference between Product D6898 and Product D3159 is the testing. Please view the sample Certificate of Analysis:
      https://www.sigmaaldrich.com/certificates/COFA/D6/D6898/D6898-BULK________SLBW8354__.pdf
      https://www.sigmaaldrich.com/certificates/sapfs/PROD/sap/certificate_pdfs/COFA/Q14/D3159-BULKSLCQ1911.pdf

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