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A7646

Sigma-Aldrich

Apyrase from potatoes

ATPase ≥60 units/mg protein, lyophilized powder (partially purified)

Synonyme(s) :

Adénosine 5′-diphosphatase, Adénosine 5′-triphosphatase

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About This Item

Numéro CAS:
9000-95-7
Numéro de classification (Commission des enzymes):
3.6.1.5 (BRENDA, IUBMB)
Numéro CE :
232-569-8
Numéro MDL:
MFCD00130542
Code UNSPSC :
12352204
Nomenclature NACRES :
NA.54

Source biologique

potato

Type

Grade III

Forme

lyophilized powder (partially purified)

ATPase activity

≥60 units/mg protein

Poids mol.

~49 kDa by gel filtration

Composition

Protein, 30-60%

Solubilité

H2O: soluble 10 mg/mL, slightly hazy

Application(s)

diagnostic assay manufacturing

Activité étrangère

Acid Phosphatase ≤5% of base activity

Température de stockage

−20°C

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Application

Apyrase is used to hydrolyze nucleoside triphosphates and diphosphates. For hydrolysis of organic di and triphosphates, the optimal pH is 6, and for inorganic substrates, the optimal pH is 5.1 Apyrase, from Sigma, has been used in inhibition studies of platelet-aggregation. The enzyme has been used to assess the possibility of ATP involvement in intestinal lamina propria Th17 cell differentiation.
Au moins deux isoenzymes sont présentes dans diverses souches de S. tuberosum : l′une avec un rapport ATPase/ADPase élevé (∼ 10) et l′autre avec un rapport faible (∼ 1).
Réaction : ATP → ADP+Pi → AMP+2Pi.

Actions biochimiques/physiologiques

Apyrase is found in all eukaryotes and some prokaryotes. Apyrase, from potato, has a crucial role in regulating growth and development. Apyrase is involved in the inactivation of synaptic ATP as a neurotransmitter following nerve stimulation and in the inhibition of ADP induced platelet aggregation to prevent thrombosis. Divalent metal ions are required for activity and best activity is observed with calcium ion at 5 mM. The molecular weight of the protein is found to be approximately 45 kDa.

Conditionnement

Sold on the basis of ATPase units

Autres remarques

Mixture of high and low ratio isoenzymes.

Qualité

Enthält Säurephosphatase.

Définition de l'unité

One unit will liberate 1.0 μmole of inorganic phosphate from ATP or ADP per min at pH 6.5 at 30 °C.

Pictogrammes

Health hazard
GHS08

Mention d'avertissement

Danger

Mentions de danger

H334

Conseils de prudence

P261 - P284 - P501

Classification des risques

Resp. Sens. 1

Code de la classe de stockage

11 - Combustible Solids

Classe de danger pour l'eau (WGK)

WGK 1

Point d'éclair (°F)

Not applicable

Point d'éclair (°C)

Not applicable

Équipement de protection individuelle

Eyeshields, Gloves, type N95 (US)

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Yen-Ta Chen et al.
Journal of tissue engineering and regenerative medicine, 13(12), 2155-2168 (2019-09-11)
This study tested the hypothesis that preactivated and disaggregated shape-changed platelet (PreD-SCP) therapy significantly protected rat kidney from ischemia-reperfusion (IR) injury. Adult-male Sprague-Dawley rats (n = 24) were equally categorized into Groups 1 (sham-operated control [SC]), 2 (SC + PreD-SCP)
H J Kruse et al.
Arteriosclerosis and thrombosis : a journal of vascular biology, 14(11), 1821-1828 (1994-11-01)
The blocker of receptor-mediated calcium entry SK&F 96365 was used to evaluate the contribution of calcium influx to the formation of biologically active endothelial prostanoids and endothelium-derived relaxing factor (EDRF). SK&F 96365 inhibited histamine-stimulated calcium entry into human umbilical vein
Marko Gosak et al.
Life (Basel, Switzerland), 11(5) (2021-05-01)
Ca2+ homeostasis and signaling disturbances are associated with lens pathophysiology and are involved in cataract formation. Here, we explored the spatiotemporal changes in Ca2+ signaling in lens epithelial cells (LECs) upon local mechanical stimulation, to better understand the LECs' intercellular
Xiao-Jing Kuang et al.
Theranostics, 12(8), 3703-3718 (2022-06-07)
Rationale: Stress is a major risk factor for the development of depression. However, the underlying molecular mechanisms of stress vulnerability in depression are largely uncharacterized. Methods: P2X2 receptors (a major receptor for gliotransmitter-ATP) in the medial prefrontal cortex (mPFC) were
Flow-cytometric analysis of mouse platelet function.
Bernhard Nieswandt et al.
Methods in molecular biology (Clifton, N.J.), 272, 255-268 (2004-07-01)
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