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Atto 425-Biotin

BioReagent, suitable for fluorescence, ≥95.0% (HPCE)

Synonyme(s) :

Biotin-Atto 425

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A propos de cet article

Formule empirique (notation de Hill) :
C37H53N5O7S
Poids moléculaire :
711.91
UNSPSC Code:
12352108
eCl@ss:
34058011
PubChem Substance ID:
NACRES:
NA.32
MDL number:


product line

BioReagent

assay

≥95.0% (HPCE)

form

powder

manufacturer/tradename

ATTO-TEC GmbH

fluorescence

λex 437 nm; λem 483 nm in 0.1 M phosphate pH 7.0

suitability

suitable for fluorescence

storage temp.

−20°C

SMILES string

CCOC(=O)C1=Cc2cc3C(C)CC(C)(C)N(CCCC(=O)NCCCCCNC(=O)CCCCC4SCC5NC(=O)NC45)c3cc2OC1=O

InChI

1S/C37H53N5O7S/c1-5-48-34(45)26-19-24-18-25-23(2)21-37(3,4)42(28(25)20-29(24)49-35(26)46)17-11-14-32(44)39-16-10-6-9-15-38-31(43)13-8-7-12-30-33-27(22-50-30)40-36(47)41-33/h18-20,23,27,30,33H,5-17,21-22H2,1-4H3,(H,38,43)(H,39,44)(H2,40,41,47)

InChI key

LPVYZZMUDCZDQO-UHFFFAOYSA-N

General description

Atto 425 is a new label with high quantum yield (0.90) as well as sufficient stoke′s shift (excitation maximum 436 nm, emission maximum 484 nm). It is optimized for excitation with argon laser, and is characterized by high photostability. It does nearly not show cis-trans-isomerization, which limits brightness and reproducibility for many other dyes.

Application

Atto fluorescent labels are designed for high sensitivity applications, including single molecule detection. Atto labels have rigid structures that do not show any cis-trans-isomerization. Thus these labels display exceptional intensity with minimal spectral shift on conjugation. Atto 425 biotin is suitable for fluorescence labeling of avidin/streptavidin conjugated molecules.

Legal Information

This product is for Research use only. In case of intended commercialization, please contact the IP-holder (ATTO-TEC GmbH, Germany) for licensing.

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Cet article
410512856252595
suitability

suitable for fluorescence

suitability

suitable for fluorescence

suitability

suitable for fluorescence

suitability

suitable for fluorescence

fluorescence

λex 437 nm; λem 483 nm in 0.1 M phosphate pH 7.0

fluorescence

-

fluorescence

-

fluorescence

λex 511 nm; λem 533 nm±10 nm in PBS, pH 7.4

form

powder

form

-

form

-

form

solid

assay

≥95.0% (HPCE)

assay

≥90% (HPLC)

assay

≥90% (HPLC), ≥90% (degree of coupling )

assay

≥90.0% (HPLC)

storage temp.

−20°C

storage temp.

−20°C

storage temp.

−20°C

storage temp.

−20°C

product line

BioReagent

product line

BioReagent

product line

BioReagent

product line

-


Classe de stockage

11 - Combustible Solids

wgk

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, type N95 (US)



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Atto dyes are a series of fluorescent dyes that meet the critical needs of modern fluorescent technologies.


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H Denk
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Praus, P., et al.
Spectroscopy: An International Journal, 24, 303-307 (2010)
Richard S Agnes et al.
Journal of the American Chemical Society, 132(17), 6075-6080 (2010-04-13)
A fluorescent sensor of protein kinase activity has been developed and used to characterize the compartmentalized location of cAMP-dependent protein kinase activity in mitochondria. The sensor functions via a phosphorylation-induced release of a quencher from a peptide-based substrate, producing a



Numéro d'article de commerce international

RéférenceGTIN
28616-1MG-F04061823353595

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