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IP05

Protein G Plus/Protein A Agarose Suspension

Protein G PLUS/Protein A-Agarose mixture specifically formulated for immunoprecipitation.

Synonyme(s) :

Affinity resin, Protein G/Agarose

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ConditionnementRéférenceDisponibilitéPrix
1.5 mL
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198,00 $

A propos de cet article

NACRES:
NA.56
UNSPSC Code:
41116133

198,00 $

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form

slurry (Liquid)

contains

≤0.1% sodium azide as preservative

manufacturer/tradename

Calbiochem®

storage condition

do not freeze

technique(s)

protein purification: suitable

suitability

suitable for microbiology

shipped in

wet ice

storage temp.

2-8°C

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Cet article
IP04IP02IP10
Protein G Plus/Protein A Agarose Suspension Protein G PLUS/Protein A-Agarose mixture specifically formulated for immunoprecipitation.

Millipore

IP05

Protein G Plus/Protein A Agarose Suspension

Protein G Plus-Agarose Suspension Protein G PLUS agarose suspension specifically formulated for immunoprecipitation.

Millipore

IP04

Protein G Plus-Agarose Suspension

Protein A Agarose Suspension Protein A Agarose Suspension designed for immunoprecipitation applications

Millipore

IP02

Protein A Agarose Suspension

Protein G Plus/Protein A-Agarose A mixture of Protein G PLUS and Protein A covalently conjugated to agarose. Useful for purification of IgG from biological fluids.

Millipore

IP10

Protein G Plus/Protein A-Agarose

technique(s)

protein purification: suitable

technique(s)

immunoprecipitation (IP): suitable

technique(s)

-

technique(s)

protein purification: suitable

form

slurry (Liquid)

form

slurry (Liquid)

form

slurry (Liquid)

form

slurry (Liquid)

suitability

suitable for microbiology

suitability

suitable for immunoprecipitation

suitability

-

suitability

suitable for microbiology

storage temp.

2-8°C

storage temp.

2-8°C

storage temp.

2-8°C

storage temp.

2-8°C

shipped in

wet ice

shipped in

wet ice

shipped in

wet ice

shipped in

wet ice

contains

≤0.1% sodium azide as preservative

contains

≤0.1% sodium azide as preservative

contains

≤0.1% sodium azide as preservative

contains

≤0.1% sodium azide as preservative

General description

Designed for immunoprecipitation applications. This product is blocked with BSA to reduce non-specific binding and cannot be used for purification.
Protein G PLUS/Protein A-Agarose mixture specifically formulated for immunoprecipitation.

Application

Immunoprecipitation (see comments)

Physical form

33% slurry in PBS.

Other Notes

Agarose solution is supplied ready to use. Protein G Plus/Protein A Agarose immunoprecipitation reagent is blocked with BSA and should not be used for immunoglobulin purification or covalent cross-linking. For immunoprecipitation reactions 15 µl of solution per µg primary antibody is recommended. Preclearing will minimize extra bands resulting from nonspecific precipitation. To preclear, add to the sample 20 µl of agarose conjugate and 1 µg of normal IgG from the same species as the immunoprecipitating antibody. When immunoblotting is used for detection, some secondary antibodies can react nonspecifically with BSA or other proteins present at high concentrations in the sample. This can be eliminated by reducing the concentration of secondary antibody.

Legal Information

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Toxicity: Standard Handling (A)

Classe de stockage

11 - Combustible Solids

wgk

WGK 1

flash_point_f

Not applicable

flash_point_c

Not applicable

Certificats d'analyse (COA)

Recherchez un Certificats d'analyse (COA) en saisissant le numéro de lot du produit. Les numéros de lot figurent sur l'étiquette du produit après les mots "Lot" ou "Batch".

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Autophagy, 15(9), 1495-1505 (2019-03-02)
Several studies have shown that dysfunction of macroautophagy/autophagy is associated with many human diseases, including neurodegenerative disease and cancer. To explore the molecular mechanisms of autophagy, we performed a cell-based functional screening with SH-SY5Y cells stably expressing GFP-LC3, using an
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Journal of the American Society of Nephrology : JASN, 28(8), 2337-2352 (2017-03-02)
The rapid growth of an aging population creates challenges regarding age-related diseases, including AKI, for which both the prevalence and death rate increase with age. The molecular mechanism by which the aged kidney becomes more susceptible to acute injury has
Lixuan Zhan et al.
Journal of neuroinflammation, 18(1), 97-97 (2021-04-22)
Our previous study indicated that hypoxic preconditioning reduced receptor interacting protein (RIP) 3-mediated necroptotic neuronal death in hippocampal CA1 of adult rats after transient global cerebral ischemia (tGCI). Although mixed lineage kinase domain-like (MLKL) has emerged as a crucial molecule
Abdulaziz Asiri et al.
Pathology international, 69(7), 381-391 (2019-07-11)
Cten is an oncogene promoting EMT in many signaling pathways, namely through Snail. We investigated whether Cten function could be mediated through Src. Cten levels were modulated by forced expression in HCT116 and gene knockdown in SW620 CRC (colorectal cancer)
Yingying Qu et al.
Molecular cancer, 13, 95-95 (2014-04-30)
Grb2 (Growth factor receptor-bound protein 2) is a key adaptor protein in maintaining the ERK activity via linking Sos1 (Son of sevenless homolog 1) or other proteins to activated RTKs, such as EGFR. Currently, little knowledge is available concerning the
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Contenu apparenté

Agarose Beads for Immunoprecipitation and Antibody Purification

We manufacture IP and antibody purification agarose beads that purify immunoglobulins and IgG fractions. Our kits also provide a total antibody purification process

Numéro d'article de commerce international

RéférenceGTIN
IP05-1.5ML04055977220223

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