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P5985

Protease from Bacillus sp.

Name: Protease from <I>Bacillus</I> sp.
Brand: SIGMA

liquid, ≥16 U/g

Synonyme(s) :

Everlase 16.0 L

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A propos de cet article

UNSPSC Code:

12352204

EC Number:

232-752-2

NACRES:

NA.54

MDL number:

MFCD00132092

Specific activity:

≥16 U/g

Biological source:

Bacillus sp.

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Propriétés

biological source

Bacillus sp.

Quality Level

200

form

liquid

specific activity

≥16 U/g

mol wt

20--30 kDa

storage temp.

2-8°C

Description

General description

Proteases are ubiquitously found in nature and microbes are the desired source for these enzymes. They are classified into four classes namely aspartic, serine, cysteine and metalloproteases.

Application

Protease from Bacillus sp. has been used to determine its proteolytic activity by spectrophotometric method.

Biochem/physiol Actions

Protease catabolizes proteins by hydrolysis of peptide bonds. Proteases are inactivated by serine active-site inhibitors, such as phenylmethylsulfonyl fluoride (PMSF) and diisopropylfluorophosphate. Proteases, secreted from Bacillus sp., typically have molecular weights ranging from 20,000 to 30,000. They are typically stabilized by Ca²⁺ and have high isoelectric points. It is highly soluble in detergents at a wide range of pH and temperatures. Protease improves healing process by efficiently removing necrotic materials from wounds. It is extensively used in laundry detergents to remove protein based stains from clothing. In addition, protease is also used in food, pharmaceutical, leather and silk industries. It is a key constituent in biopharmaceutical products including contact-lens enzyme cleaners and enzymatic deriders. Proteases also aid in various physiological processes such as protein turnover, digestion, blood coagulation, fertilization and cell differentiation. It also facilitates growth, cell signaling, the immune response, and apoptosis.

Legal Information

A product of Novozyme Corp.

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Cet article	P3111	P5860	P0029
Sigma-Aldrich P5985 Protease from Bacillus sp.	Sigma-Aldrich P3111 Protease from Bacillus sp.	Sigma-Aldrich P5860 Protease from Bacillus sp.	Sigma-Aldrich P0029 Protease from Bacillus sp.
specific activity ≥16 U/g	specific activity ≥16 U/g	specific activity ≥8 U/g	specific activity -
biological source Bacillus sp.	biological source Bacillus sp.	biological source -	biological source Bacillus sp.
form liquid	form liquid	form liquid	form granular
mol wt 20--30 kDa	mol wt 20-30 kDa	mol wt 20--30 kDa	mol wt -
storage temp. 2-8°C	storage temp. 2-8°C	storage temp. 2-8°C	storage temp. 2-8°C
Quality Level 200	Quality Level 200	Quality Level 200	Quality Level 200