Phospho(enol)pyruvic acid trisodium salt hydrate

Name: Phospho(enol)pyruvic acid trisodium salt hydrate
Brand: SIGMA

≥97% (enzymatic), powder

Synonyme(s) :

2-(Phosphonooxy)-2-propenoic acid trisodium salt, PEP

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A propos de cet article

Formule linéaire :

C₃H₂O₆PNa₃· x H₂O

Numéro CAS:

5541-93-5

Poids moléculaire :

233.99 (anhydrous basis)

UNSPSC Code:

12352204

PubChem Substance ID:

24898810

NACRES:

NA.32

MDL number:

MFCD00150734

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Propriétés

Nom du produit

Phospho(enol)pyruvic acid trisodium salt hydrate, ≥97% (enzymatic)

Quality Segment

200

assay

≥97% (enzymatic)

form

powder

solubility

water: 100 mg/mL, clear to slightly hazy, colorless to very faintly yellow

storage temp.

−20°C

SMILES string

[Na].OC(=O)C(=C)OP(O)(O)=O

InChI

1S/C3H5O6P.Na.H/c1-2(3(4)5)9-10(6,7)8;;/h1H2,(H,4,5)(H2,6,7,8);;

InChI key

KZPUEZVJSLLABH-UHFFFAOYSA-N

Description

Application

Phospho(enol)pyruvic acid trisodium salt hydrate has been used in:

several enzyme activity assays, such as PRPP synthetase (PRPPS) assay, ribokinase (RK) assay, adenine phosphoribosyltransferase (APRT) assay
in the reaction mixture for enzyme kinetic assay to determine platelet-derived growth factor receptor α (PDGFRA) kinetic parameters
as a component in buffer B to react with adenosine diphosphate (ADP) to form adenosine triphosphate (ATP) for energy charge measurement

Biochem/physiol Actions

Phospho(enol)pyruvic acid (PEP) is involved in glycolysis and gluconeogenesis. In glycolysis, PEP is metabolized by pyruvate kinase to yield pyruvate. In plants, PEP is involved in the formation of aromatic amino acids as well as in the carbon fixation pathway.

Phosphoenolpyruvic acid plays a major role in the initial step of glycolysis by acting as an inhibitor of hexokinase, phosphoglucose isomerase, phosphofructokinase and aldolase.