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F0628

Ferredoxin-NADP+ Reductase from Spinacia oleracea (spinach)

lyophilized powder, ≥15 units/mg solid, secondary activity: ≥10 units/mg solid NADPH diaphorase

Synonyme(s) :

Ferridoxin-NADP+ oxidoreductase

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A propos de cet article

Numéro CAS:
9029-33-8
Numéro CE :
1.18.1.2 (BRENDA, IUBMB)
UNSPSC Code:
12352204
NACRES:
NA.54
MDL number:
MFCD00131050
Specific activity:
≥15 units/mg solid
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form

lyophilized powder

Quality Segment

200

specific activity

≥15 units/mg solid

foreign activity

NADH diaphorase activity ≤10 units/mg solid

storage temp.

−20°C

General description

Ferredoxin-NADP+ Reductase is a ferredoxin-soluble partner with a molar ratio to PSI of 0.9 and 3.

Application

Ferredoxin-NADP+ Reductase was used in in vitro ferredoxin-dependent desaturation of fatty acids in cyanobacterial thylakoid membranes.[1] It was also used to regulate glyceraldehyde-3-phosphate dehydrogenase.[2]
Ferredoxin-NADP+ Reductase from Spinacia oleracea (spinach) has been used to test the effect of potential protein partners on the activity of the glyceraldehyde-3-phosphate dehydrogenase (GAPDH)−CP12 preparation. It has also been used in standard assay conditions for phycocyanobilin:ferredoxin oxidoreductase (PcyA) enzyme assay.

Biochem/physiol Actions

Ferredoxin-NADP+ reductase catalyzes the reversible conversion of reduced ferredoxin to oxidized ferredoxin during photosynthesis.[3] Ferredoxin-NADP(H) reductase constitutes a family of hydrophilic FAD-containing monomeric enzymes that deliver NADPH or low potential one-electron donors to redox-based metabolisms in plastids, mitochondria, and bacteria.[4]
Ferredoxin-NADP+ reductase catalyzes the reversible conversion of reduced ferredoxin to oxidized ferredoxin during photosynthesis[3]. Ferredoxin-NADP(H) reductase constitutes a family of hydrophilic FAD-containing monomeric enzymes that deliver NADPH or low potential one-electron donors to redox-based metabolisms in plastids, mitochondria, and bacteria.

Other Notes

One unit will reduce 1.0 millimole of cytochrome C per min at pH 7.5 at 25 °C in the presence of spinach ferredoxin and NADP.

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Cet article
F3013P9752F5875
Ferredoxin-NADP+ Reductase from Spinacia oleracea (spinach) lyophilized powder, ≥15 units/mg solid, secondary activity: ≥10 units/mg solid NADPH diaphorase

Sigma-Aldrich

F0628

Ferredoxin-NADP+ Reductase from Spinacia oleracea (spinach)

Ferredoxin from Spinacia oleracea (spinach) lyophilized powder, Ferredoxin ≥15 wt. %

Sigma-Aldrich

F3013

Ferredoxin from Spinacia oleracea (spinach)

Phosphoriboisomerase from spinach Type I, partially purified powder, ≥40 units/mg protein (biuret)

Sigma-Aldrich

P9752

Phosphoriboisomerase from spinach

Ferredoxin from Spinacia oleracea (spinach) buffered aqueous solution

Sigma-Aldrich

F5875

Ferredoxin from Spinacia oleracea (spinach)

specific activity

≥15 units/mg solid

specific activity

-

specific activity

≥40 units/mg protein (biuret)

specific activity

-

form

lyophilized powder

form

lyophilized powder

form

partially purified powder

form

buffered aqueous solution

storage temp.

−20°C

storage temp.

−20°C

storage temp.

−20°C

storage temp.

−70°C

Quality Level

200

Quality Level

200

Quality Level

200

Quality Level

200

foreign activity

NADH diaphorase activity ≤10 units/mg solid

foreign activity

-

foreign activity

-

foreign activity

-

Classe de stockage

11 - Combustible Solids

wgk

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, type N95 (US)

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Certificats d'analyse (COA)

Lot/Batch Number
0000416094
0000416094
0000316871
0000316871
SLCN7462

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Numéro d'article de commerce international

RéférenceGTIN
F0628-5UN04061832729435
F0628-1UN04061833611319
F0628-10UN04061832729411

Questions

1–2 of 2 Questions  

  1. Hi, how can I prepare a stock solution with the 1 U vial? It's not very clear for me in the specification sheet. I need a 1 uM final concentration of reductase for my assay, how do I prepare a stock solution of it?

    1 answer

    1. The molecular weight of this enzyme has not been analyzed. The enzymatic assay states a solubility of 1 mg/ ml in water.
      Immediately prior to pipetting into cuvette, prepare a solution at 1 mg / mL in cold purified water. Place on ice.
      This solution is stable for a maximum of thirty minutes.

      Helpful?

  2. Hi there, could you please provide the specific methodology to extract this lyophilized powder from the vial? I didn't know how to extract this ferredoxin-NADP+ reductase from the vial

    1 answer

    1. The vials contain less than 1mg solid so reconstituting the whole vial is recommended.

      Helpful?

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