C7594
Casein Blocking Buffer
for Northern and Southern blotting, powder blend
Synonyme(s) :
blocking buffer
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About This Item
Produits recommandés
Qualité
for molecular biology
Niveau de qualité
Forme
powder blend
Technique(s)
Southern blotting: suitable
pH
6.8-7.2(reconstituted per directions)
Solubilité
H2O: soluble, faintly yellow (dissolves in 5-10 minutes.)
Température de stockage
room temp
Catégories apparentées
Description générale
Casein is a milk-derived substance routinely used in blocking buffers for Western, Southern and Northern Blot procedures. Blocking agents are used to reduce non-specific binding of detection reagents to the membranes.
Application
Suitable as a blocking Buffer for Western, Southern and Northern Blot membranes. Blocked membranes can then be used in either chemiluminescent, colorimetric, or DIG detection assays.
Caractéristiques et avantages
- Better blocking performance than dried milk or BSA
- Compatible with neutral and positively-charged membranes
- Compatible with chemiluminescent, colorimetric , and DIG detection assays
Notes préparatoires
Dissolve entire contents of bottle in 800 mL molecular biology grade water. Stir until well mixed, then add water to final volume of 1L. Store at 2-8°C for up to one week.
Produit(s) apparenté(s)
Réf. du produit
Description
Tarif
Code de la classe de stockage
11 - Combustible Solids
Classe de danger pour l'eau (WGK)
WGK 1
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