C4290
Butyrylcholinesterase from equine serum
lyophilized powder, ≥500 units/mg protein
Synonyme(s) :
Acylcholine acyl-hydrolase, Choline esterase, butyryl, Pseudocholinesterase
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A propos de cet article
Numéro CAS:
UNSPSC Code:
12352204
NACRES:
NA.54
EC Number:
232-579-2
MDL number:
Specific activity:
≥500 units/mg protein
Biological source:
equine serum
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equine serum
Quality Level
form
lyophilized powder
specific activity
≥500 units/mg protein
composition
Protein, ≥10%
storage temp.
−20°C
Application
Butyrylcholinesterase (BChE) from equine serum has been used:
- to determine the inhibitory concentration of bupivacaine on butyrylcholinesterase
- in acetylcholinesterase (AChE)/BChE activity assay to determine the inhibitory activity of benzothiazole-piperazine compounds[1]
Selective inhibition of BChE activity can be used in the detection of organophosphates. Its use in the treatment of organophosphate toxicity shows promise. There is a correlation between the level of BChE in human blood and degree of protection against potentially toxic nerve agents.[2] There has also been interest in the roles of cholinesterases with regard to Alzheimer′s disease. Investigations into selective inhibitors may provide a clearer picture of the physiological role of BChE in both healthy and diseased individuals.[3] The enzyme has been used to test bupivacaine as an inhibitor of butyrylcholinesterase during acetylcholinesterase assay using cerebrospinal fluid.[4]
Biochem/physiol Actions
Butyrylcholinesterase (BChE) is a serine hydrolase that is structurally similar to acetylcholinesterase (AChE), but differs in substrate specificities and inhibitor sensitivities. BChE can, unlike AChE, efficiently hydrolyze larger esters of choline such as butyrylcholine and benzoylcholine. The enzyme is a tetrameric glycoprotein with four equal subunits (110 kDa each). The enzyme is activated by Ca2+ and Mg2+ and the activity is constant over the pH range 6.0-8.0. It is inhibited by Betaine, nicotine, organophosphates, carbamates.
Physical form
Highly purified, lyophilized powder containing buffer salts
Analysis Note
Protein determined by biuret
Other Notes
One unit will hydrolyze 1.0 μmole of butyrylcholine to choline and butyrate per min at pH 8.0 at 37 °C. The activity obtained using butyrylcholine as substrate is ~2.5 times that obtained using acetylcholine.
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Cet article | |||
|---|---|---|---|
| biological source equine serum | biological source - | biological source - | biological source - |
| specific activity ≥500 units/mg protein | specific activity ≥900 units/mg protein | specific activity ≥900 units/mg protein | specific activity ≥10 units/mg protein |
| form lyophilized powder | form lyophilized powder | form lyophilized powder | form lyophilized powder |
| storage temp. −20°C | storage temp. −20°C | storage temp. −20°C | storage temp. −20°C |
| Quality Level 200 | Quality Level 300 | Quality Level 400 | Quality Level 200 |
| composition Protein, ≥10% | composition Protein, ≥10% | composition - | composition Protein, ≥60% |
signalword
Danger
hcodes
pcodes
Hazard Classifications
Resp. Sens. 1
Classe de stockage
11 - Combustible Solids
wgk
WGK 3
flash_point_f
Not applicable
flash_point_c
Not applicable
ppe
Eyeshields, Gloves, type N95 (US)
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Contenu apparenté
QC Methods
Stefano Cinti et al.
Nature protocols, 14(8), 2437-2451 (2019-07-05)
Despite substantial advances in sensing technologies, the development, preparation, and use of self-testing devices is still confined to specialist laboratories and users. Decentralized analytical devices will enormously impact daily lives, enabling people to analyze diverse clinical, environmental, and food samples
Determination of thyroxine binding globulin.
Bergmeyer, H.U
Methods of Enzymatic Analysis, 2, 833-833 (1974)
W H Kluge et al.
BMC biochemistry, 2, 17-17 (2002-01-22)
Most test systems for acetylcholinesterase activity (E.C.3.1.1.7.) are using toxic inhibitors (BW284c51 and iso-OMPA) to distinguish the enzyme from butyrylcholinesterase (E.C.3.1.1.8.) which occurs simultaneously in the cerebrospinal fluid. Applying Ellman's colorimetric method, we were looking for a non-toxic inhibitor to
Numéro d'article de commerce international
| Référence | GTIN |
|---|---|
| C4290-1KU | 04061833491966 |