A7345
Anti-V5 Agarose Affinity Gel antibody produced in mouse
purified immunoglobulin, clone V5-10
Synonyme(s) :
Monoclonal Anti-V5
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A propos de cet article
Conjugate:
agarose conjugate
Clone:
V5-10, monoclonal
Application:
IP, affinity chromatography
Citations:
161
Le tarif et la disponibilité ne sont pas disponibles actuellement.
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Laissez-nous vous aiderbiological source
mouse
Quality Level
conjugate
agarose conjugate
antibody form
purified immunoglobulin
antibody product type
primary antibodies
clone
V5-10, monoclonal
analyte chemical class(es)
proteins (V5)
technique(s)
affinity chromatography: suitable, immunoprecipitation (IP): suitable
capacity
2.5 nmol/mL, gel (V5 fusion protein)
shipped in
wet ice
storage temp.
2-8°C
General description
Anti-V5 Agarose Affinity Gel is made of purified immunoglobulin fraction of monoclonal anti-V5 isolated from ascites fluid of the V5-10 hybridoma coupled to cyanogen bromide-activated beaded agarose. The purified monoclonal antibody is immobilized on agarose.
The Anti-V5 Agarose Affinity Gel is composed of the monolonal anti-V5 antibody conjugated to cyanogen bromide-activated beaded agarose. The antibody recognizes the V5-tagged recombinant fusion proteins expressed in transfected mammalian cells.
Application
Biochem/physiol Actions
Recognizes V5 Tag (GKPIPNPLLGLDST) fusion proteins
Recombinant DNA technology enables the attachment of genes of interest to specific sequences or genes that can provide ′affinity handles′ designed to enable the selective identification and purification of the protein of interest.[6] The addition of a V5 tag sequence (GKPIPNPLLGLDST)[6] to a given gene creates a stable fusion product that does not appear to interfere with the bioactivity of the protein, or with the biodistribution of the tagged product. Many recombinant proteins have been engineered to express a short sequence derived from the V5 from simian virus known as V5-tag.[7] Anti-“tag” agarose conjugates may facilitate the detection, isolation and purification of these proteins.[7]
Physical form
Suspension of beaded agarose (1:1 v/v) in 0.01 M phosphate buffered saline, containing 15 mM sodium azide.
Preparation Note
For continuous use and extended storage, store at 2 °C to 8 °C. Do not freeze.
Disclaimer
Unless otherwise stated in our catalog, our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Cet article | |||
|---|---|---|---|
| antibody form purified immunoglobulin | antibody form purified immunoglobulin | antibody form purified immunoglobulin | antibody form purified immunoglobulin |
| biological source mouse | biological source mouse | biological source mouse | biological source mouse |
| clone V5-10, monoclonal | clone P5D4, monoclonal | clone V5-10, monoclonal | clone HA-7, monoclonal |
| conjugate agarose conjugate | conjugate agarose conjugate | conjugate CY3 conjugate | conjugate agarose conjugate |
| shipped in wet ice | shipped in wet ice | shipped in wet ice | shipped in wet ice |
| technique(s) affinity chromatography: suitable, immunoprecipitation (IP): suitable | technique(s) immunoprecipitation (IP): suitable, protein purification: suitable | technique(s) immunocytochemistry: 2.0-4.0 μg/mL | technique(s) - |
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Classe de stockage
10 - Combustible liquids
wgk
WGK 3
flash_point_f
Not applicable
flash_point_c
Not applicable
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Contenu apparenté
Lynnette C Goatley et al.
Journal of virology, 85(7), 3294-3305 (2011-01-21)
The African swine fever virus (ASFV)-encoded CD2v transmembrane protein is required for the hemadsorption of red blood cells around infected cells and is also required for the inhibition of bystander lymphocyte proliferation in response to mitogens. We studied the expression
Q Meng et al.
Analytical biochemistry, 421(2), 351-361 (2011-11-01)
Quantitation of individual monoclonal antibodies (mAbs) within a combined antibody drug product is required for preclinical and clinical drug development, including pharmacokinetic (PK), toxicology, stability, and biochemical characterization studies of such drugs. We have developed an antitoxin, XOMA 3AB, consisting
Arko Dasgupta et al.
PLoS genetics, 11(5), e1005215-e1005215 (2015-05-16)
Most organisms on earth sense light through the use of chromophore-bearing photoreceptive proteins with distinct and characteristic photocycle lengths, yet the biological significance of this adduct decay length is neither understood nor has been tested. In the filamentous fungus Neurospora
Numéro d'article de commerce international
| Référence | GTIN |
|---|---|
| A7345-1ML | 04061833384046 |