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A6680

N-Acetylneuraminic Acid Aldolase from microorganisms

Name: N-Acetylneuraminic Acid Aldolase from microorganisms
Brand: SIGMA

lyophilized powder, ≥20 units/mg protein (biuret)

Synonyme(s) :

N-Acetylneuraminate Pyruvate Lyase, N-Acetylneuraminic Acid Lyase, NANA Aldolase, Sialic Aldolase

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A propos de cet article

Numéro CAS:

9027-60-5

NACRES:

NA.32

UNSPSC Code:

12352204

Beilstein/REAXYS Number:

2697172

MDL number:

MFCD00131656

Numéro CE :

4.1.3.3 (BRENDA, IUBMB)

Specific activity:

≥20 units/mg protein (biuret)

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Propriétés

form

lyophilized powder

Quality Segment

200

specific activity

≥20 units/mg protein (biuret)

mol wt

~98 kDa

storage temp.

−20°C

Description

General description

Isoelectric point: 4.6 ± 0.1
Michaelis constant: 2.5 x 10‾³M (N-Acetylneuraminic acid)
Structure: 3 subunits (approx. 35,000) per mol of enzyme
Inhibitors: p-Chloromercuribenzoate, sodium dodecyl sulfact, Hg⁺⁺, Ag⁺
Optimum pH: 7.5– 8.0
Optimum temp: 70°C
pH Stability: pH 6.0–9.0 (10°C, 25hr)
Thermal stability: Below 65°C (pH 7.5, 30 min)

Application

This enzyme is useful for enzymatic determination of N-acetylneuraminic acid and sialic acid when
coupled with the related enzymes in clinical analysis.
For industrial use, this enzyme is useful for enzymatic synthesis of sialic acid.

Used in the Sialic Acid Quantification Kit, SIALIC-Q

Physical form

Lyophilized powder containing mannitol and EDTA

Other Notes

One unit will release 1.0 μmole of pyruvate from NANA per min at pH 7.7 at 37 °C.

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Cet article	A2714	S1826	A8811
Sigma-Aldrich A6680 N-Acetylneuraminic Acid Aldolase from microorganisms	Sigma-Aldrich A2714 Aldolase from rabbit muscle	Sigma-Aldrich S1826 Sialic Acid Aldolase from Escherichia coli K12	Sigma-Aldrich A8811 Aldolase from rabbit muscle
specific activity ≥20 units/mg protein (biuret)	specific activity ≥8.0 units/mg protein	specific activity ≥3.0 units/mg protein	specific activity 10-20 units/mg protein
form lyophilized powder	form lyophilized powder	form lyophilized powder	form ammonium sulfate suspension
storage temp. −20°C	storage temp. −20°C	storage temp. −20°C	storage temp. 2-8°C
mol wt ~98 kDa	mol wt -	mol wt 33.4 kDa	mol wt -
Quality Level 200	Quality Level 300	Quality Level 200	Quality Level 200