FD40S

Isothiocyanate-dextrane marqué à la fluorescence

average mol wt 40,000

• Synonyme(s) : FITC-dextrane
• Numéro CAS: 60842-46-8
• Numéro MDL: MFCD00131092
• Code UNSPSC : 12352201
• Nomenclature NACRES : NA.25

Propriétés

• Source biologique: synthetic (organic)
• Conjugué: FITC conjugate
• Forme: powder
• Poids mol.: average mol wt 40,000
• Ampleur du marquage: 0.003-0.020 mol FITC per mol glucose
• Couleur: yellow to dark orange
• Solubilité: H₂O: soluble 25 mg/mL, clear to slightly hazy, yellow to deep orange
• Température de stockage: 2-8°C

Description

Description générale

Dextran is a polymer of anhydroglucose. It is composed of approximately 95% α-D-(166) linkages. The remaining (163) linkages account for the branching of dextran.[1][2] Native dextran has been found to have a molecular weight (MW) in the range of 9 million to 500 million.[3] FITC-dextran has an excitation maximum of 490 nm. It has a pH range of 5 to 9. [4]

Application

Dextrane marqué à l′isothiocyanate de fluorescéine pour utilisation potentielle dans les études de perfusion chez l′animal.

Fluorescein isothiocyanate–dextran (FITC-dextran, fluorescein-dextran, FITC) was used in the following processes:

• Fluorescein-dextran microscopy.[5][6][7]
• To evaluate the in vivo pulmonary vascular permeability in polymorphonuclear leukocyte α-defensins expressing transgenic mice.[8]
• To quantify endocytic activity by flow cytometry.[9]
• To label α-lactalbumin and ribonuclease.[10]

FITC-dextran is suitable for extensive microcirculation and cell permeability research utilizing microfluorimetry. It is also suitable for the study of plant cell wall porosity and capillary permeability.

Liaison

Similar to FD-40, but produced by Sigma.

Notes préparatoires

Sigma typically tests the solubility of FITC dextrans in water at concentrations at or above 25 mg/ml. Solutions should be protected from light. In vivo, FITC-dextran is stable for more than 24 hours.

Autres remarques

Native dextran has been found to have a molecular weight (MW) in the range of 9 million to 500 million. Lower MW dextrans will exhibit slightly less branching and have a more narrow range of MW distribution. Dextrans with MW greater than 10,000 behave as if they are highly branched. As the MW increases, dextran molecules attain greater symmetry. Dextrans with MW of 2,000 to 10,000, exhibit the properties of an expandable coil. At MW below 2,000, dextran is more rod-like. The MW of dextran is measured by one or more of the following methods: low angle laser light scattering, size exclusion chromatography, copper-complexation and anthrone reagent colorometric reducing-end sugar determination and viscosity.

To gain a comprehensive understanding of our extensive range of Dextrans for your research, we encourage you to visit our Carbohydrates Category page.

Informations sur la sécurité

• Pictogrammes: GHS07
• Mention d'avertissement: Warning
• Mentions de danger: H315,H319,H335
• Conseils de prudence: P261 - P264 - P271 - P280 - P302 + P352 - P305 + P351 + P338
• Classification des risques: Eye Irrit. 2 - Skin Irrit. 2 - STOT SE 3
• Organes cibles: Respiratory system
• Code de la classe de stockage: 11 - Combustible Solids
• Classe de danger pour l'eau (WGK): WGK 3
• Point d'éclair (°F): Not applicable
• Point d'éclair (°C): Not applicable
• Équipement de protection individuelle: Eyeshields, Gloves, type N95 (US)