D4941

Anti-phospho-DAP-Kinase (pSer³⁰⁸) antibody, Mouse monoclonal

clone DKPS308, purified from hybridoma cell culture

• Synonyme(s) : Anti-DAPK, Anti-ROCO3
• Numéro MDL: MFCD04040944
• Code UNSPSC : 12352203
• Nomenclature NACRES : NA.41

Propriétés

• Source biologique: mouse
• Niveau de qualité: 200
• Conjugué: unconjugated
• Forme d'anticorps: purified from hybridoma cell culture
• Type de produit anticorps: primary antibodies
• Clone: DKPS308, monoclonal
• Forme: buffered aqueous solution
• Poids mol.: antigen ~160 kDa
• Espèces réactives: human
• Technique(s): immunoprecipitation (IP): suitable; indirect ELISA: suitable; microarray: suitable; western blot: 1-2 μg/mL using 293T (human embryonal kidney) cells transfected with DAP-kinase expression vector.
• Isotype: IgG1
• Numéro d'accès UniProt: P53355
• Conditions d'expédition: dry ice
• Température de stockage: −20°C
• Modification post-traductionnelle de la cible: phosphorylation (pSer308)
• Informations sur le gène: human ... DAPK1(1612)

Description

Description générale

Monoclonal Anti-phospho DAP-Kinase (pSer308) (mouse IgG1 isotype) is derived from the DKPS308 hybridoma produced by the fusion of mouse myeloma cells and splenocytes from BALB/c mice immunized with a phosphopeptide of human DAP-kinase, conjugated to KLH. Death Associated Protein Kinase (DAPK) is characterized with a multidomain structure including subdomain typical of serine/threonine kinases, a Ca²⁺/calmodulin regulatory domain, eight ankyrin repeats followed by two P-loop motifs and a typical death domain module. In addition, it also contains two auto-inhibitory domains, one of them Ca²⁺/calmodulin dependent. In the absence of this latter domain, DAPK is constitutively active.

Immunogène

Phosphopeptide corresponding to amino acids 303-312 (pSer³⁰⁸) of human DAP-kinase, conjugated to KLH.

Application

Anti-phospho-DAP-Kinase (pSer308) antibody, Mouse monoclonal has been used in:

• western blot assay
• immunocytochemistry
• enzyme linked immunosorbent assay (ELISA)
• immunoprecipitation

Monoclonal anti-phospho-DAP-kinase (pSer³⁰⁸) antibody can be used in indirect ELISA, immunoblotting and immunoprecipitation. It can also be used in western blotting.

Actions biochimiques/physiologiques

Death Associated Protein Kinase (DAPK) plays crucial role in programmed cell death as well as in autophagy. DAPK activity is regulated by phosphorylation. Autophosphorylation at Ser³⁰⁸ on the calmodulin regulatory domain negatively regulate DAPK activity. This autophosphorylation, which occurs in cells at the basal state, lowers the affinity of DAPK for calmodulin and thus the kinase is inactive. Under some apoptotic conditions DAPK undergoes dephosphorylation. Consequently, it binds to calmodulin with higher affinity, becomes activated, phosphorylates its downstream substrate proteins, and mediates apoptosis. Monoclonal anti-phospho-DAP-kinase (pSer308) antibody can be used to study the mechanism of DAPK activation in apoptosis. It can also be used in microarray.

Forme physique

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.

Clause de non-responsabilité

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Informations sur la sécurité

• Code de la classe de stockage: 12 - Non Combustible Liquids
• Classe de danger pour l'eau (WGK): nwg
• Point d'éclair (°F): Not applicable
• Point d'éclair (°C): Not applicable