D2192
Desthiobiotin polyethyleneoxide Iodoacetamide
≥90% (HPLC)
Synonyme(s) :
Desthiobiotin PEO iodoacetamide
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A propos de cet article
Formule empirique (notation de Hill) :
C21H38IN5O6
Poids moléculaire :
583.46
UNSPSC Code:
12352200
PubChem Substance ID:
MDL number:
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≥90% (HPLC)
form
powder
solubility
H2O: 10 mg/mL
storage temp.
2-8°C
SMILES string
CC1NC(=O)NC1CCCCCC(=O)NCCC(=O)NCCOCCOCCNC(=O)CI
InChI
1S/C21H38IN5O6/c1-16-17(27-21(31)26-16)5-3-2-4-6-18(28)23-8-7-19(29)24-9-11-32-13-14-33-12-10-25-20(30)15-22/h16-17H,2-15H2,1H3,(H,23,28)(H,24,29)(H,25,30)(H2,26,27,31)
InChI key
DBOWCCSTSCXTEL-UHFFFAOYSA-N
Application
Especially useful for cysteine labeling in many proteomics applications such as peptide mapping and mass spectrometry.
Features and Benefits
- Easily eluted from streptavidin or avidin affinity matrices under mild conditions
- Incorportates a 16 atom hydrophilic spacer
- Typically coupled to sulfhydryl groups at pH 7.5 - 8.5
Other Notes
Sulfhydryl specific, water soluble biotinylation reagent.
signalword
Warning
hcodes
Hazard Classifications
Eye Irrit. 2 - Skin Irrit. 2 - STOT SE 3
Classe de stockage
13 - Non Combustible Solids
wgk
WGK 3
flash_point_f
Not applicable
flash_point_c
Not applicable
ppe
dust mask type N95 (US), Eyeshields, Gloves
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Contenu apparenté
Instructions
Michael B Goshe et al.
Journal of proteome research, 2(2), 153-161 (2003-04-29)
The ability to identify and quantitate integral membrane proteins is an analytical challenge for mass spectrometry-based proteomics. The use of surfactants to solubilize and facilitate derivatization of these proteins can suppress peptide ionization and interfere with chromatographic separations during microcapillary
Advances in proteome analysis by mass spectrometry.
T J Griffin et al.
The Journal of biological chemistry, 276(49), 45497-45500 (2001-10-05)
T P Conrads et al.
Analytical chemistry, 73(9), 2132-2139 (2001-05-17)
We describe the combined use of 15N-metabolic labeling and a cysteine-reactive biotin affinity tag to isolate and quantitate cysteine-containing polypeptides (Cys-polypeptides) from Deinococcus radiodurans as well as from mouse B16 melanoma cells. D. radiodurans were cultured in both natural isotopic