C40

Catalase from bovine liver

lyophilized powder, ≥10,000 units/mg protein

• Synonyme(s) : H₂O₂:H₂O₂ oxidoreductase
• Numéro CAS: 9001-05-2
• Numéro de classification (Commission des enzymes): 1.11.1.6 (BRENDA, IUBMB)
• Numéro CE : 232-577-1
• Numéro MDL: MFCD00081483
• Code UNSPSC : 12352204
• eCl@ss : 32160410
• Nomenclature NACRES : NA.54

Propriétés

• Forme: lyophilized powder
• Niveau de qualité: 400
• Activité spécifique: ≥10,000 units/mg protein
• Poids mol.: tetramer ~250 kDa
• Contient: ≤0.2 wt. % Thymol
• Point isoélectrique: 5.4
• Solubilité: 50 mM potassium phosphate buffer, pH 7.0: soluble 2.0 mg/mL
• Numéro d'accès UniProt: P00432
• Conditions d'expédition: wet ice
• Température de stockage: −20°C
• InChI: 1S/C9H10O3/c1-2-12-9(11)7-3-5-8(10)6-4-7/h3-6,10H,2H2,1H3
• Clé InChI: NUVBSKCKDOMJSU-UHFFFAOYSA-N
• Informations sur le gène: cow ... CAT(280743)

Description

Description générale

Research Area: Cell Signaling

Catalase from bovine liver is a tetramer consisting of 4 equal subunits each with a 60 kDa molecular weight. Each of these subunits contains iron bound to a protoheme IX group. The enzyme will also strongly bind to NADP, where NADP and the heme group are within 13.7 angstroms.

Application

Catalase acts as a natural antioxidant to study the roles of reactive oxygen species in gene expression and apoptosis. It has also been used to protect against oxidative damage to proteins, lipids, and nucleic acids. Industrially, catalzes have been used to remove hydrogen peroxide added to milk and cheese, in textile bleaching, and to examine its positive effects on the viability of DNA-repair mutants of E. coli.

Catalase from bovine liver may be used:

• to prepare H₂O₂-O₂ based biocathode for applications in glucose biofuel cells
• to study the kinetic properties and storage stability of catalase immobilized on to florisil
• in glutathione-mediated superoxide generation in an aqueous solution

Actions biochimiques/physiologiques

Catalase activity is constant over the pH range of 4.0-8.5. The enzyme activity is inhibited by 3-amino-1-H-1,2,4 triazole, cyanide, azide, hydroxylamine, cyanogen bromide, 2-mercaptoethanol, dithiothreitol, dianisidine, and nitrate. Incubation of catalase with ascorbate or ascorbate/Cu²⁺ results in degradation of the catalase molecule.

Catalase, an antioxidant enzyme found in all aerobic organisms, catalyzes the degradation of hydrogen peroxide, a byproduct of metabolic processes, into less harmful water and oxygen. It can also react with alkylhydrogen peroxides, such as methylperoxide and ethylperoxide and the second H₂O₂ molecule can be replaced by methanol, ethanol, propanol, formate and nitrate as a hydrogen donor. Catalase enzyme uses either iron (Fe) or manganese (Mn) as cofactor, and are classified as Fe-CAT or Mn-CAT.

Attention

Solutions of catalse should not be frozen. Frozen solution will result in a 50-70% loss of activity.

Définition de l'unité

One unit will decompose 1.0 μmole of H₂O₂ per min at pH 7.0 at 25 °C, while the H₂O₂ concentration falls from 10.3 to 9.2 mM, measured by the rate of decrease of A₂₄₀.

Autres remarques

The protein content is determined by the Biuret method. In the product specifications, a protein content >= 70% is guaranteed. The impurities are not analyzed. The only other content specification is the concentration of Thymol, <= 0.2% wt.

Informations sur la sécurité

• Pictogrammes: GHS08
• Mention d'avertissement: Danger
• Mentions de danger: H334
• Conseils de prudence: P261 - P284 - P501
• Classification des risques: Resp. Sens. 1
• Code de la classe de stockage: 11 - Combustible Solids
• Classe de danger pour l'eau (WGK): WGK 1
• Point d'éclair (°F): Not applicable
• Point d'éclair (°C): Not applicable
• Équipement de protection individuelle: Eyeshields, Gloves, type N95 (US)