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SeqPlex RNA Amplification Kit

For use with high throughput sequencing technologies

Sinónimos:

WGA kit, Whole transcriptome amplification

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Tamaño de envaseSKUDisponibilidadPrecio
10 reactions
Comprobar disponibilidad del carrito
$1,080.00
50 reactions
Comprobar disponibilidad del carrito
$4,900.00
500 reactions
Comprobar disponibilidad del carrito
$24,550.00

Acerca de este artículo

NACRES:
NA.55
UNSPSC Code:
41121800

$1,080.00


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Quality Level

technique(s)

whole genome amplification: suitable

dilution

(WTA)

input

purified RNA

shipped in

wet ice

storage temp.

−20°C

General description

The SeqPlex RNA Amplification Kit for whole transcriptome amplification (WTA) is designed to facilitate next-generation sequencing (NGS) from small quantities or from degraded/highly fragmented RNA (e.g. RNA from formalin-fixed paraffin-embedded (FFPE) tissue samples). The SeqPlex kit allows the user to pre-amplify these and other small quantity/highly fragmented RNA samples for input into a NGS workflow. It also facilitates the amplification of non-polyA tailed RNA isolated from tissue, cultured cells, formalin-fixed samples, or serum while maintaining patterns of differential expression found in the unamplified sample.

Application

SeqPlex RNA Amplification Kit has been used to amplify poly(A)-selected RNA.[1][2]

Features and Benefits

  • Low quantities of total RNA random priming technology amplifies fragmented or intact RNA from all sources including FFPE and RIP.
  • Semi-degenerate library primer design for more complete transcriptome coverage and efficient priming.
  • No need to fragment DNA before sequencing.
  • Amplifies ds-cDNA in 8 hours or less.
  • Compatible with all next generation sequencing platforms except Pacific Bioscience.

Other Notes

SEQR-500RXN is manufactured on-demand. Contact us for more information.

Legal Information

SeqPlex is a trademark of Sigma-Aldrich Co. LLC

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Este artículo
SEQXEWTA2WTA1
technique(s)

whole genome amplification: suitable

technique(s)

whole genome amplification: suitable

technique(s)

whole genome amplification: suitable

technique(s)

whole genome amplification: suitable

storage temp.

−20°C

storage temp.

−20°C

storage temp.

−20°C

storage temp.

−20°C

Quality Level

200

Quality Level

200

Quality Level

200

Quality Level

200

shipped in

wet ice

shipped in

wet ice

shipped in

wet ice

shipped in

wet ice

dilution

(WTA)

dilution

(WGA)

dilution

(WTA)

dilution

(WTA)

input

purified RNA

input

purified DNA

input

purified RNA

input

purified DNA


Clase de almacenamiento

11 - Combustible Solids

flash_point_f

Not applicable

flash_point_c

Not applicable

wgk

WGK 3



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Protocolos

The SeqPlex RNA Amplification kit provides a method for amplification of total RNA or isolated mRNA prior to entry into the workflows of the commonly used deep sequencing platforms.

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SKUGTIN
SEQR-500RXN04061837018114
SEQR-10RXN04061837018107
SEQR-50RXN04061837018121

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