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F7425

ANTI-FLAG® antibody produced in rabbit

affinity isolated antibody, buffered aqueous solution

ANTI-FLAG® antibody produced in rabbit

Sinónimos:

Anti-ddddk, Anti-dykddddk

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Tamaño de envaseSKUDisponibilidadPrecio
0.2 mg

Disponible para enviar HOYdesdeMILWAUKEE

$632.00

Acerca de este artículo

UNSPSC Code:
12352203
NACRES:
NA.32
MDL number:
MFCD02262912
Conjugate:
unconjugated
Clone:
polyclonal
Application:
DB, IF, IP, WB CL
Citations:
3023

$632.00

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biological source

rabbit

Quality Level

200

conjugate

unconjugated

antibody form

affinity isolated antibody

antibody product type

primary antibodies

clone

polyclonal

form

buffered aqueous solution

species reactivity

all

concentration

~0.8 mg/mL

technique(s)

dot blot: 1-2.5 μg/mL, immunoprecipitation (IP): 4-8 μg using amino terminal FLAG-BAP fusion protein from E. coli crude lysate, indirect immunofluorescence: 5-10 μg/mL using 293T cells transfected with a plasmid encoding FLAG-JNK, western blot (chemiluminescent): 1-2.5 μg/mL using an E. coli periplasmic extract expressing an N-terminal FLAG fusion protein

isotype

IgG

immunogen sequence

DYKDDDDK

shipped in

dry ice

storage temp.

−20°C

General description

El anticuerpo policlonal de afinidad anti-FLAG de conejo ANTI-FLAG reconoce el epítopo FLAG situado en las proteínas de fusión FLAG. Este anticuerpo reacciona con los extremos N-terminal, Met-N-terminal, C-terminal de las proteínas de fusión FLAG.
El epítopo FLAG es una proteína de ocho aminoácidos con un sitio de escisión por enterocinasa.

Immunogen

FLAG; secuencia peptídica DYKDDDDK

Application

El anticuerpo reconoce el epítopo FLAG situado en las proteínas de fusión etiquetadas con FLAG en los extremos N-terminal o C-terminal mediante la aplicación de ensayos de inmunotransferencia por puntos (dot blot), inmunotransferencia, inmunoprecipitación e inmunocitoquímica.
Conozca más detalles del producto en nuestro portal de la aplicación FLAG®.

Biochem/physiol Actions

El epítopo FLAG desempeña un papel crucial en la purificación mediante inmunoafinidad de las proteínas de fusión.

Physical form

Disolución en disolución salina tamponada con fosfato 0,01 M, pH 7,4, que contiene seroalbúmina bovina al 1 % y acida sódica 15 mM.

Preparation Note

Purificado mediante cromatografía de afinidad en una columna portadora del péptido inmunizante.

Other Notes

Ganador del premio CiteAb 2024 como proveedor de éxito en la investigación del Parkinson

Legal Information

FLAG is a registered trademark of Merck KGaA, Darmstadt, Germany

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Este artículo
F2555F3165SAB4200119
ANTI-FLAG® antibody produced in rabbit affinity isolated antibody, buffered aqueous solution

Millipore

F7425

ANTI-FLAG® antibody produced in rabbit

Monoclonal ANTI-FLAG® antibody produced in rabbit clone SIG1-25, ascites fluid

Sigma-Aldrich

F2555

Monoclonal ANTI-FLAG® antibody produced in rabbit

ANTI-FLAG® M2 antibody, Mouse monoclonal clone M2, purified immunoglobulin (Purified IgG1 subclass), buffered aqueous solution (10 mM sodium phosphate, 150 mM NaCl, pH 7.4, containing 0.02% sodium azide)

Sigma-Aldrich

F3165

ANTI-FLAG® M2 antibody, Mouse monoclonal

Monoclonal Anti-FLAG-Peroxidase antibody produced in rat 2-4 mg/mL, clone 6F7, purified immunoglobulin

Sigma-Aldrich

SAB4200119

Monoclonal Anti-FLAG-Peroxidase antibody produced in rat

species reactivity

all

species reactivity

-

species reactivity

all

species reactivity

all

clone

polyclonal

clone

SIG1-25, monoclonal

clone

M2, monoclonal

clone

6F7, monoclonal

conjugate

unconjugated

conjugate

unconjugated

conjugate

unconjugated

conjugate

peroxidase conjugate

biological source

rabbit

biological source

rabbit

biological source

mouse

biological source

rat

antibody form

affinity isolated antibody

antibody form

ascites fluid

antibody form

purified immunoglobulin (Purified IgG1 subclass)

antibody form

purified immunoglobulin

shipped in

dry ice

shipped in

dry ice

shipped in

dry ice

shipped in

dry ice

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Clase de almacenamiento

12 - Non Combustible Liquids

flash_point_f

Not applicable

flash_point_c

Not applicable

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Certificados de análisis (COA)

Lot/Batch Number
0000485141
0000485141
0000422515
0000422515
0000395474

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SKUGTIN
F7425-.2MG04061838255501
1 Review This action will navigate to reviews.

Questions

1–9 of 9 Questions  

  1. Can this AB (F7425) used for mouse 30um thick section. mouse will be perfused with 4% PFA and than cryopreserved. thanks

    1 answer

    1. This product has been recommended for use in immunofluorescence and indirect immunofluorescence at 5-10 μg/mL using 293T cells transfected with a plasmid encoding FLAG-JNK. It has not been tested for use in IHC applications. The end-user will need to optimize the assay. Please see the link below to review the product datasheet:
      https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/product/documents/207/353/f7425dat-ms.pdf

      In general, antibodies work well on 5-15 uM sections, for 30 uM sections, blocking /permeabilization steps and antibody dilution, the incubation time will need to be optimized by end user. Please see the links below to review an alternative anti-Flag antibody that may be more suitable for this application. Note that this product is a mouse monoclonal antibody:
      https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/product/documents/175/747/f1804bul-ms.pdf
      https://www.sigmaaldrich.com/product/sigma/f1804

      Helpful?

  2. Is there a recommended isotype control for this antibody?

    1 answer

    1. Any rabbit IgG such as product I5006 would be an appropriate isotype control for this antibody.

      Helpful?

  3. Is there any references for the use of Product F7465, ANTI-FLAG® from rabbit, in immunohistochemistry or western blotting?

    1 answer

    1. K. Schafer and T. Braun, Monoclonal Anti-FLAG Antibodies React with a New Isoform of Rat Mg++ Dependent Protein Phosphatase. Biochemical and Biophysical Research Communications, 207, 708.

      Helpful?

  4. Is Product F7425, ANTI-FLAG® from rabbit, a monoclonal or polyclonal?

    1 answer

    1. F7425 is a polyclonal antibody from rabbit.

      Helpful?

  5. What is the Department of Transportation shipping information for this product?

    1 answer

    1. Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product.

      Helpful?

  6. What is the concentration of Product F7425, ANTI-FLAG® from rabbit?

    1 answer

    1. The concentration is 0.9 mg protein per ml.

      Helpful?

  7. What are the positive controls that can be used with Product F7425, ANTI-FLAG® from rabbit?

    1 answer

    1. The positive controls that can be used are as follows:Amino-terminal FLAG-BAP fusion protein, Product No. P7582Carboxy-terminal FLAG-BAP fusion protein, Product No. P7457Amino-terminal Met-FLAG-BAP fusion protein, Product No. P5975.

      Helpful?

  8. How to get rid of background when using Product F7465, ANTI-FLAG® from rabbit,?

    1 answer

    1. To reduce non-specific background on tissue staining, a small amount of Triton X-100 (Product No. X100 or T8787), can be added to the wash buffers and antibody incubation buffers. Try a Triton X-100 concentration of 0.1% to start. Tween 20 (Product No. P5927) can also be used.

      Helpful?

  9. How much Product F7425, ANTI-FLAG® from rabbit, should be used in western blotting and ELISA applications?

    1 answer

    1. For a Western blot from a PAGE run (preferably) under denaturing conditions, a 1:400 dilution of this antibody should be used.  A 1-10 μg/ml concentration should be used when using thie antibody in an ELISA application.

      Helpful?

Reviews

Active Filters

  1. Nottingham
    • Review 1
    • Votes 0
    5 out of 5 stars.

    Western blotting

    I HAVE USED THIS ANTIBODY ALOT DURING MY RESEARCH. AND IT HAS WORKED PERFECTLY.

    Helpful?