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05019

7-Diethylamino-3-[N-(2-maleimidoethyl)carbamoyl]coumarin

BioReagent, suitable for fluorescence, ≥97.0% (HPLC)

Sinónimos:

MDCC

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Tamaño de envaseSKUDisponibilidadPrecio
5 mg

Disponible para enviar HOYdesdeMILWAUKEE

$154.00

Acerca de este artículo

Fórmula empírica (notación de Hill):
C20H21N3O5
Número CAS:
Peso molecular:
383.40
UNSPSC Code:
12352116
PubChem Substance ID:
NACRES:
NA.32
MDL number:

$154.00


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product line

BioReagent

Quality Segment

assay

≥97.0% (HPLC)

suitability

suitable for fluorescence

SMILES string

CCN(CC)c1ccc2C=C(C(=O)NCCN3C(=O)C=CC3=O)C(=O)Oc2c1

InChI

1S/C20H21N3O5/c1-3-22(4-2)14-6-5-13-11-15(20(27)28-16(13)12-14)19(26)21-9-10-23-17(24)7-8-18(23)25/h5-8,11-12H,3-4,9-10H2,1-2H3,(H,21,26)

InChI key

IXQPRUQVJIJUEB-UHFFFAOYSA-N

Application

Thiol-reactive probe for protein labelling
7-Diethylamino-3-[N-(2-maleimidoethyl)carbamoyl]coumarin is utilized as a fluoresencent biological sensing device . Used for real-time measurements for the release of inorganic phosphates during enzymatic reaction when MDCC is conjugated to a mutant phosphate-binding protein . Also, utilized for intramolecular fluorescence energy transfer (FRET) experiments .

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Este artículo
G762700062107M9307
suitability

suitable for fluorescence

suitability

-

suitability

-

suitability

-

Quality Level

100

Quality Level

200

Quality Level

100

Quality Level

200

assay

≥97.0% (HPLC)

assay

≥90%

assay

-

assay

-

product line

BioReagent

product line

-

product line

-

product line

-


pictograms

Exclamation mark

signalword

Warning

hcodes

Hazard Classifications

Acute Tox. 4 Oral

Clase de almacenamiento

11 - Combustible Solids

wgk

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

dust mask type N95 (US), Eyeshields, Gloves



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Maria M Martinez-Senac et al.
Biochemistry, 44(51), 16967-16976 (2005-12-21)
RecG is a DNA helicase involved in the repair of damage at a replication fork and catalyzes the reversal of the fork to a point beyond the damage in the template strand. It unwinds duplex DNA in reactions that are
Martin R Webb
Molecular bioSystems, 3(4), 249-256 (2007-03-21)
Biosensors are becoming widely used both in basic research and in screening assays and reagentless sensors with fluorescent reporter groups attached to proteins form one class. This article describes the development of sensors for two small molecules, driven in particular
Lyndon L E Salins et al.
Sensors and actuators. B, Chemical, 97(1), 81-89 (2004-03-06)
This work explores the potential use of a member of the periplasmic family of binding proteins, the phosphate binding protein (PBP), as the biorecognition element in a sensing scheme for the detection of inorganic phosphate (Pi). The selectivity of this



Número de artículo de comercio global

SKUGTIN
150215-5G04061831818727
1483185-10MG04061833852651
PHR3071-25MG04065266899955
150215-1G04061825583037
05019-1MG-F04061823353243
05019-5MG-F04061833488553

Questions

  1. What do the excitation/emission peaks of this dye look like?

    1 answer
    1. The product specification requires an emission wavelength range of 460-470nm. The product is not screened for excitation wavelength, however it is expected to fall within a range of 416 to 425 nm. The compound is typically used for real-time measurements for the release of inorganic phosphates during enzymatic reaction when MDCC is conjugated to a mutant phosphate-binding protein . Please see the link below to review a publication that may be of interest:
      https://pubs.acs.org/doi/10.1021/bi9804277

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