The band detected on western blot is typically between 100-120 kDa. Please see a link to the reference below.
https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7425076/#MOESM2
AB5302
Anti-Amyloid Precursor Protein (APP) Antibody
CHEMICON®, rabbit polyclonal
Sinónimos:
APP
Iniciar sesión para ver los precios por organización y contrato.
Seleccione un Tamaño
Cambiar Vistas
| Tamaño de envase | SKU | Disponibilidad | Precio |
|---|---|---|---|
| 100 μL | Comprobar disponibilidad del carrito | $519.00 |
Acerca de este artículo
UNSPSC Code:
12352203
eCl@ss:
32160702
NACRES:
NA.41
Clone:
polyclonal
Species reactivity:
monkey, mouse, human, bovine
Application:
ICC, IHC, IP, WB
Citations:
7
$519.00
Comprobar disponibilidad del carrito
Está disponible un anticuerpo recombinante y sin conservantes para su diana. Pruebe ZRB1151
Servicio técnico
¿Necesita ayuda? Nuestro equipo de científicos experimentados está aquí para ayudarle.
Permítanos ayudarleNombre del producto
Anti-Amyloid Precursor Protein Antibody, KPI Domain, serum, Chemicon®
biological source
rabbit
Quality Segment
antibody form
serum
antibody product type
primary antibodies
clone
polyclonal
species reactivity
monkey, mouse, human, bovine
manufacturer/tradename
Chemicon®
technique(s)
immunocytochemistry: suitable, immunohistochemistry: suitable, immunoprecipitation (IP): suitable, western blot: suitable
NCBI accession no.
UniProt accession no.
shipped in
dry ice
target post-translational modification
unmodified
Gene Information
human ... APP(351)
Immunogen
APP.
Application
Anti-Amyloid Precursor Protein Antibody, KPI Domain is an antibody against Amyloid Precursor Protein for use in IP, WB, IC, IH.
Western blot: 1:250 - 1:1,000
Immunoprecipitation: Requires reductions and alkylation of disulfide bonds.
Immunocytochemistry: On Cos7 and NteraZ cell lines, requires reduction and alkylation of disulphide bonds.
Immunohistochemistry: Works on formaldehyde fixed, paraffin embedded tissue, requires reduction and alkylation of disulphide bonds or microwaving.
Optimal working dilutions must be determined by end user.
Reduction /Alkylation protocol:
To reduce incubate the slide (Blot) in TBS (PBS) + 50mM 2-mercaptoethanol at 37°C for 3 h in the dark. Wash twice with TBS, then incubate in TBS (PBS) + 20 mg/ ml of Iodoacetic acid for 30 min at room temperature. Wash twice with TBS (PBS), block and process as usual.
Immunoprecipitation: Requires reductions and alkylation of disulfide bonds.
Immunocytochemistry: On Cos7 and NteraZ cell lines, requires reduction and alkylation of disulphide bonds.
Immunohistochemistry: Works on formaldehyde fixed, paraffin embedded tissue, requires reduction and alkylation of disulphide bonds or microwaving.
Optimal working dilutions must be determined by end user.
Reduction /Alkylation protocol:
To reduce incubate the slide (Blot) in TBS (PBS) + 50mM 2-mercaptoethanol at 37°C for 3 h in the dark. Wash twice with TBS, then incubate in TBS (PBS) + 20 mg/ ml of Iodoacetic acid for 30 min at room temperature. Wash twice with TBS (PBS), block and process as usual.
Biochem/physiol Actions
Amyloid Precursor Protein (APP), KPI Domain.
Legal Information
CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany
1 of 1
Este artículo | |||
|---|---|---|---|
| antibody form serum | antibody form serum | antibody form serum | antibody form purified immunoglobulin |
| biological source rabbit | biological source rabbit | biological source rabbit | biological source mouse |
| Quality Level 100 | Quality Level 100 | Quality Level 100 | Quality Level 100 |
| Gene Information human ... APP(351) | Gene Information human ... APP(351) | Gene Information human ... APP(351) | Gene Information human ... APP(351) |
| UniProt accession no. | UniProt accession no. | UniProt accession no. | UniProt accession no. |
| species reactivity monkey, mouse, human, bovine | species reactivity human, monkey, mouse | species reactivity human, mouse | species reactivity human |
Still not finding the right product?
Explore all of our products under Anti-Amyloid Precursor Protein Antibody, KPI Domain
— o —
Pruebe nuestra Herramienta de selección de productos para limitar sus opciones
wgk
WGK 2
flash_point_f
Not applicable
flash_point_c
Not applicable
Clase de almacenamiento
12 - Non Combustible Liquids
Certificados de análisis (COA)
Busque Certificados de análisis (COA) introduciendo el número de lote del producto. Los números de lote se encuentran en la etiqueta del producto después de las palabras «Lot» o «Batch»
¿Ya tiene este producto?
Encuentre la documentación para los productos que ha comprado recientemente en la Biblioteca de documentos.
Número de artículo de comercio global
| SKU | GTIN |
|---|---|
| AB5302 | 04053252335037 |
-
Hello, I bought this antibody and I run wstern-blot. I would like to know what is the molecular wight for the KPI domain? Thanks
1 answer-
Helpful?
-