This product is stable as a powder, kept refrigerated. See the certificate of analysis for a lot-specific recommended retest date. It is also stable as a crystalline suspension of 3.2 M (NH4)2SO4 solution containing potassium phosphate buffer, pH 6.0 (see Product No. P6140). It is reasonably stable in solution in phosphate buffer. We have found that at 10 mg/mL in 0.1 phosphate buffer, pH 6.0, solutions kept frozen at -20°C lose less than 2% of their activity per week.
P8375
Peroxidasa from horseradish
Type VI, essentially salt-free, lyophilized powder, ≥250 units/mg solid (using pyrogallol)
Sinónimos:
Donante:peróxido de hidrógeno oxidorreductasa, Peroxidasa de rábano picante
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| Tamaño de envase | SKU | Disponibilidad | Precio |
|---|---|---|---|
| 1000 units | Disponible para enviar HOYdesdeCarlos Spegazzini | US$ 89,30 | |
| 2000 units | Disponible para enviar HOYdesdeCarlos Spegazzini | US$ 146,00 | |
| 5000 units | Comprobar disponibilidad del carrito | US$ 153,00 | |
| 10000 units | Comprobar disponibilidad del carrito | US$ 356,00 | |
| 25000 units | Comprobar disponibilidad del carrito | US$ 608,00 | |
| 100000 units | Comprobar disponibilidad del carrito | US$ 2.580,00 | |
| 250000 units | Comprobar disponibilidad del carrito | US$ 4.950,00 |
Acerca de este artículo
Número CAS:
eCl@ss:
32160410
UNSPSC Code:
12352204
NACRES:
NA.54
EC Number:
232-668-6
MDL number:
Specific activity:
≥250 units/mg solid (using pyrogallol)
US$ 89,30
PRECIOS SIN IMPUESTOS NACIONALES
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Servicio técnico
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Type VI
Quality Segment
form
essentially salt-free, lyophilized powder
specific activity
≥250 units/mg solid (using pyrogallol)
mol wt
~44 kDa
solubility
0.1 M phosphate buffer: soluble 10 mg/mL, clear, orange to red (pH 6.0)
absorbance ratio
RZ 2.5-4.0
application(s)
diagnostic assay manufacturing
storage temp.
2-8°C
SMILES string
[O+H2]O[O-]
InChI
1S/H2O3/c1-3-2/h1-2H
InChI key
JSPLKZUTYZBBKA-UHFFFAOYSA-N
General description
La peroxidasa de rábano picante se aísla de las raíces de rábano picante (Amoracia rusticana) y pertenece al grupo de ferroprotoporfirina de las peroxidasas. La HRP es un polipéptido monocatenario que contiene cuatro puentes bisulfuro. Es una glucoproteína que contiene un 18 % de carbohidratos. La composición de los carbohidratos consiste en galactosa, arabinosa, xilosa, fucosa, manosa, manosamina y galactosamina dependiendo de la isoenzima específica. Su peso molecular (~44 kDa) incluye la cadena polipeptídica (33 890 dalton), la hemina más Ca2+ (~700 dalton) y los carbohidratos (~9 400 dalton). Existen al menos siete isoenzimas de la HRP. El punto isoeléctrico de las isoenzimas de la peroxidasa de rábano picante oscila entre 3,0 y 9,0.
Application
Horseradish peroxidase (HRP) is isolated from horseradish roots (Amoracia rusticana) and belongs to the ferroprotoporphyrin group of peroxidases.. It is used in biochemistry applications such as western blots, ELISA and Immunohistochemistry. Horseradish peroxidase is used to amplify a weak signal and increase detectability of a target molecule, such as a protein. Product P8375, type VI, is an essentially salt free lyophilized powder. It is commonly used to determine amounts of glucose and peroxides in solution. It has been used to study sensory input of cervical spinal cord neurons[1].
The enzyme has been used to develop a thermostable soybean peroxidase-based biosensor by cross-linking and electrically ′wiring′ the enzyme through a redox-conducting hydrogel to a glassy carbon electrode. It has also been used to assay the oxidation of low density lipoprotein.[2]
Biochem/physiol Actions
When incubated with a substrate, horseradish peroxidase produces a coloured, fluorimetric, or luminescent derivative of the labeled molecule, allowing quantification. Horseradish peroxidase has been shown to slightly reduce the level of inhibition in a cydAB mutant. Known inhibitors are sodium azide, cyanide, L-cystine, dichromate, ethylenethiourea, hydroxylamine, sulfide, vanadate, p-aminobenzoic acid, and Cd2+, Co2+, Cu2+, Fe3+, Mn2+, Ni2+, and Pb2+ ions.
HRP readily combines with hydrogen peroxide (H2O2) and the resultant [HRP-H2O2] complex can oxidize a wide variety of hydrogen donors. The optimal pH is 6.0-6.5 and the enzyme is most stable in the pH range of 5.0-9.0. HRP can be conjugated to antibodies by several different methods including glutaraldehyde, periodate oxidation, through disulfide bonds, and also via amino and thiol directed cross-linkers. It is smaller and more stable than the enzyme labels β-galactosidase and alkaline phosphatase and hence, it is the most desired label. Also, its glycosylation leads to lower non-specific binding.
Analysis Note
La RZ (Reinheitszahl) es la relación de absorbancia A403/A275 determinada a 0,5-1,0 mg/ml en agua desionizada. Es una medida del contenido de hemina, no de la actividad enzimática. Incluso preparaciones con RZ elevada pueden tener una actividad enzimática baja.
Other Notes
Una unidad de pirogalol formará 1,0 mg de purpurogalina a partir de pirogalol en 20 segundos a pH 6,0 a 20 °C.
Véase más información sobre la peroxidasa en www.sigma-aldrich.com/enzymeexplorer.
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Este artículo | |||
|---|---|---|---|
| specific activity ≥250 units/mg solid (using pyrogallol) | specific activity ≥250 units/mg solid (using pyrogallol), 950-2000 units/mg solid (using ABTS) | specific activity 150-250 units/mg solid (using pyrogallol) | specific activity ≥50 units/mg solid (using pyrogallol) |
| form essentially salt-free, lyophilized powder | form essentially salt-free, lyophilized powder | form essentially salt-free, lyophilized powder | form essentially salt-free, lyophilized powder |
| application(s) diagnostic assay manufacturing | application(s) diagnostic assay manufacturing | application(s) diagnostic assay manufacturing | application(s) - |
| solubility 0.1 M phosphate buffer: soluble 10 mg/mL, clear, orange to red (pH 6.0) | solubility 0.1 M phosphate buffer: soluble 10 mg/mL, clear, orange to red (pH 6.0), H2O: soluble | solubility 0.1 M phosphate buffer: soluble (pH 6.0), H2O: soluble | solubility 0.1 M phosphate buffer: soluble (pH 6.0), H2O: soluble |
| storage temp. 2-8°C | storage temp. 2-8°C | storage temp. 2-8°C | storage temp. 2-8°C |
| mol wt ~44 kDa | mol wt ~44 kDa | mol wt ~44 kDa | mol wt ~44 kDa |
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signalword
Danger
hcodes
pcodes
Hazard Classifications
Resp. Sens. 1
Clase de almacenamiento
11 - Combustible Solids
wgk
WGK 1
ppe
Eyeshields, Gloves, type N95 (US)
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Protocolos
This procedure is for the determination of Peroxidase enzymatic activity using Pyrogallol as the substrate.
To standardize a procedure for the assay of Peroxidase using 2,2'-Azino-bis(3-Ethylbenzthiazoline-6-Sulfonic Acid) as a substrate.
Reinheitszahl (RZ) is the ratio of absorbance due to hemin (A403, Soret region) to absorbance due to protein (A275).
Artículos
Discover our peroxidase from horseradish enzymes, products, substrates, and inhibitors for your ELISA, immunoassay, and protein application needs.
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Número de artículo de comercio global
| SKU | GTIN |
|---|---|
| P8375-5KU | 04061835513031 |
| P8375-2KU | 04061835558827 |
| P8375-250KU | 04061835558803 |
| P8375-100KU | 04061835558780 |
| P8375-25KU | 04061835558810 |
| P8375-1KU | 04061835513024 |
| P8375-10KU | 04061835558797 |
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What can you tell me about the stability of Peroxidase from horseradish, Product P8375, (as is, in suspension and in solution)?
1 answer-
Helpful?
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How do I convert units of Peroxidase from horseradish, Product P8375, to solid?
1 answer-
The activity listed on the Certificate of Analysis for each lot can be used to convert from units to milligrams of solid.
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What is the Department of Transportation shipping information for this product?
1 answer-
Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product.
Helpful?
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What is the optimal pH range of Peroxidase from horseradish, Product P8375?
1 answer-
The optimal pH range for product P8375 is 6.0 - 6.5.
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