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C8868

Cholesterol Oxidase from Brevibacterium

Name: Cholesterol Oxidase from <I>Brevibacterium</I>
Brand: SIGMA
Price: 293 USD

lyophilized powder, ≥50 units/mg protein, recombinant, expressed in E. coli

Sinónimos:

Cholesterol: oxygen oxidoreductase

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Tamaño de envase	SKU	Disponibilidad	Precio
100 units		Comprobar disponibilidad del carrito	US$ 293,00
500 units		Comprobar disponibilidad del carrito	US$ 990,00

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Número CAS:

9028-76-6

UNSPSC Code:

12352204

NACRES:

NA.54

EC Number:

232-842-1

MDL number:

MFCD00130783

Número CE:

1.1.3.6 (BRENDA, IUBMB)

Specific activity:

≥50 units/mg protein

Recombinant:

expressed in E. coli

US$ 293,00

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Propiedades

recombinant

expressed in E. coli

Quality Segment

200

form

lyophilized powder

specific activity

≥50 units/mg protein

mol wt

64 kDa

composition

Protein, ≥15% biuret

storage temp.

−20°C

Descripción

General description

Type II cholesterol oxidase containing FAD cofactor covalently linked to the enzyme.

Application

Cholesterol oxidase has been used in a study to demonstrate that microheterogeneity in hydrophobic-hydrophilic block copolymer systems can be exploited for immobilizing enzymes and to carry out enzymatic reactions. Cholesterol oxidase has also been used in a study that concluded that amphipaths that activate cholesterol might be useful in treating NPC disease.

Cholesterol oxidase is used to determine serum cholesterol.[1][2] The enzyme also finds application in the microanalysis of steroids in food samples and in distinguishing 3-ketosteroids from 3β-hydroxysteroids.[3] Transgenic plants expressing cholesterol oxidase are being investigated in the fight against the cotton boll weevil.[4] CHOD has also been used as a molecular probe to elucidate cellular membrane structures.[5][6]

Biochem/physiol Actions

Cholesterol oxidase (CHOD) is a monomeric flavoprotein containing FAD that catalyzes the first step in cholesterol catabolism. This bifunctional enzyme oxidizes cholesterol to cholest-5-en-3-one in an FAD-requiring step, which is then isomerized to cholest-4-en-3-one with the release of H₂O₂.[7]

Physical form

Supplied as a lyophilzed powder containing sucrose

Other Notes

One unit will convert 1.0 μmole of cholesterol to 4-cholesten-3-one per min at pH 7.5 at 25 °C. Note: 4-cholesten-3-one may undergo isomerization.

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Este artículo	C1235	C5421	C4405
Sigma-Aldrich C8868 Cholesterol Oxidase from microorganisms	Sigma-Aldrich C1235 Cholesterol Oxidase microbial	Sigma-Aldrich C5421 Cholesterol Oxidase from microorganisms	Sigma-Aldrich C4405 Choline Oxidase from Arthrobacter globiformis
specific activity ≥50 units/mg protein	specific activity ≥10 units/mg protein	specific activity ≥30 units/mg protein (biuret)	specific activity 8-20 units/mg solid
form lyophilized powder	form lyophilized powder	form aqueous solution	form lyophilized powder
recombinant expressed in E. coli	recombinant -	recombinant -	recombinant -
mol wt 64 kDa	mol wt 55 kDa	mol wt 62 kDa	mol wt 71 kDa by SDS-PAGE, 83 kDa by gel filtration
storage temp. −20°C	storage temp. −20°C	storage temp. −70°C	storage temp. −20°C
Quality Level 200	Quality Level 200	Quality Level 200	Quality Level 200