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C8058

Chondroitinase B from Flavobacterium heparinum

lyophilized powder (with BSA as stabilizer)

Sinónimos:

Chondroitin sulfate B lyase

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Tamaño de envaseSKUDisponibilidadPrecio
50 units
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US$ 942,00

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Número CAS:
52227-83-5
UNSPSC Code:
12352204
NACRES:
NA.54
MDL number:
MFCD00166444
Biological source:
bacterial (Flavobacterium heparinum)

US$ 942,00

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biological source

bacterial (Flavobacterium heparinum)

Quality Level

200

conjugate

(Glucosaminoglycan)

form

lyophilized powder (with BSA as stabilizer)

shipped in

dry ice

storage temp.

−20°C

Application

Chondroitinase B from Flavobacterium heparinum has been used in a study to assess the structural characterization and antithrombin activity of dermatan sulfate. Chondroitinase B from Flavobacterium heparinum has also been used in a study to investigate the chondroitin lyase action pattern via liquid chromatography–mass spectrometry.
The enzyme from Sigma has been used for the analysis of the frequency of iduronic acid in dermatan sulfate dodecasaccharide.[1] It has also been used to digest dermatan sufate (DS) in melanoma cells. This digestion resulted in decreased proliferation and invasiveness of tumor cells, thereby suggesting a role for DS in metastasis.[2]

Biochem/physiol Actions

Chondroitinase B degrades only chondroitin sulphate B producing oligo- and tetra-saccharides, and an unsaturated 4-sulphated disaccharide. It has an optimum temperature of 20 °C and an optimum pH of 8.0. The enzyme activity is inhibited by 50% using 0.1 M NaCI. Co2+, Fe3+ and Ba2+ also inhibit its activity.[3]

Other Notes

One unit will form 0.1 μmole of unsaturated uronic acid per hr at pH 7.5 at 25°C using chondroitin sulfate B as substrate.

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Este artículo
E2039C2780C0954
Chondroitinase B from Flavobacterium heparinum lyophilized powder (with BSA as stabilizer)

Sigma-Aldrich

C8058

Chondroitinase B from Flavobacterium heparinum

Chondroitinase AC from Flavobacterium heparinum recombinant, expressed in E. coli, ≥200 units/mg protein, For Chondroitin Sulfate Analysis

Sigma-Aldrich

E2039

Chondroitinase AC from Flavobacterium heparinum

Chondroitinase AC from Flavobacterium heparinum lyophilized powder, 0.5-1.5 units/mg protein (using chondroitin sulfate A as substrate)

Sigma-Aldrich

C2780

Chondroitinase AC from Flavobacterium heparinum

Chondroitinase C from Flavobacterium heparinum lyophilized powder, ≥200 units/mg solid

Sigma-Aldrich

C0954

Chondroitinase C from Flavobacterium heparinum

biological source

bacterial (Flavobacterium heparinum)

biological source

-

biological source

-

biological source

-

form

lyophilized powder (with BSA as stabilizer)

form

lyophilized solid

form

lyophilized powder

form

lyophilized powder

storage temp.

−20°C

storage temp.

−20°C

storage temp.

−20°C

storage temp.

−20°C

shipped in

dry ice

shipped in

dry ice

shipped in

-

shipped in

-

Quality Level

200

Quality Level

200

Quality Level

200

Quality Level

200

conjugate

(Glucosaminoglycan)

conjugate

(Glucosaminoglycan)

conjugate

(Glucosaminoglycan)

conjugate

(Glucosaminoglycan)

pictograms

Exclamation mark
GHS07

signalword

Warning

hcodes

H319

Hazard Classifications

Eye Irrit. 2

Clase de almacenamiento

13 - Non Combustible Solids

wgk

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, type N95 (US)

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Certificados de análisis (COA)

Lot/Batch Number
0000294092
0000282814
0000285693
0000269784
SLCQ4035

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Contenido relacionado

Zhenqing Zhang et al.
Analytical biochemistry, 385(1), 57-64 (2008-11-11)
Liquid chromatography-mass spectrometry was applied to determine the action pattern of different chondroitin lyases. Two commercial enzymes, chondroitinase ABC (Proteus vulgaris) and chondroitinase ACII (Arthrobacter aurescens), having action patterns previously determined by viscosimetry and gel electrophoresis were first examined. Next
Y M Michelacci et al.
The Biochemical journal, 151(1), 121-129 (1975-10-01)
A chondroitinase that degrades only chondroitin sulphate B was isolated from Flavobacterium heparinum, and separated from a constitutive chondroitinase AC also present in extracts of F. heparinum. The enzyme acts only on chondroitin sulphate B, producing oligo- and tetra-saccharides, plus
Yvette M Coulson-Thomas et al.
Journal of neuroscience methods, 171(1), 19-29 (2008-04-18)
Injury to the CNS of vertebrates leads to the formation of a glial scar and production of inhibitory molecules, including chondroitin sulphate proteoglycans. Various studies suggest that the sugar component of the proteoglycan is responsible for the inhibitory role of
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SKUGTIN
C8058-50UN04061833629376

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