B6149
5-Bromo-4-chloro-3-indolyl phosphate disodium salt
chromogenic, ≥98% (HPLC), powder
Sinónimos:
5-BCIP disodium salt, BCIP®, X-phosphate disodium salt
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| Tamaño de envase | SKU | Disponibilidad | Precio |
|---|---|---|---|
| 25 mg | Comprobar disponibilidad del carrito | US$ 92,50 | |
| 50 mg | Comprobar disponibilidad del carrito | US$ 99,90 | |
| 100 mg | Comprobar disponibilidad del carrito | US$ 149,00 | |
| 500 mg | Comprobar disponibilidad del carrito | US$ 495,00 | |
| 1 g | Comprobar disponibilidad del carrito | US$ 747,00 | |
| 5 g | Comprobar disponibilidad del carrito | US$ 3.020,00 |
Acerca de este artículo
Fórmula lineal:
C8H4BrClNO4P · 2Na
Número CAS:
Peso molecular:
370.43
UNSPSC Code:
12352204
PubChem Substance ID:
NACRES:
NA.83
MDL number:
US$ 92,50
PRECIOS SIN IMPUESTOS NACIONALES
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Permítanos ayudarleNombre del producto
5-Bromo-4-chloro-3-indolyl phosphate disodium salt, ≥98% (HPLC)
Quality Level
assay
≥98% (HPLC)
form
powder
solubility
H2O: 20 mg/mL, DMF: insoluble
storage temp.
−20°C
SMILES string
[Na+].[Na+].[O-]P([O-])(=O)Oc1c[nH]c2ccc(Br)c(Cl)c12
InChI
1S/C8H6BrClNO4P.2Na/c9-4-1-2-5-7(8(4)10)6(3-11-5)15-16(12,13)14;;/h1-3,11H,(H2,12,13,14);;/q;2*+1/p-2
InChI key
OAZUOCJOEUNDEK-UHFFFAOYSA-L
General description
Histochemical substrate for alkaline phosphatase.
Application
For the colorimetric detection of alkaline phosphatase-labeled molecules, 5-Bromo-4-chloro-3-indolyl phosphate (BCIP) and Nitro Blue Tetrazolium (NBT) are available. The BCIP/NBT substrate system is versatile and functions in a variety of applications, including Northern Southern, and Western blotting, in situ hybridization, and immunohistochemistry.
BCIP is provided in two salt forms: the disodium salt which is soluble in water and the p-toluidine form which is soluble in dimethylformamide. These salt forms may be used to prepare a stock solution that in combination with NBT and a reaction buffer, form a substrate solution for alkaline phosphatase. This substrate system, when incubated with alkaline phosphatase, produces an insoluble NBT diformazan product that is easily observable with its purple color. For added convenience, a mixture of BCIP and NBT is provided in an easily dissolvable tablet form or as a ready-to-use liquid.
BCIP is provided in two salt forms: the disodium salt which is soluble in water and the p-toluidine form which is soluble in dimethylformamide. These salt forms may be used to prepare a stock solution that in combination with NBT and a reaction buffer, form a substrate solution for alkaline phosphatase. This substrate system, when incubated with alkaline phosphatase, produces an insoluble NBT diformazan product that is easily observable with its purple color. For added convenience, a mixture of BCIP and NBT is provided in an easily dissolvable tablet form or as a ready-to-use liquid.
For the colorimetric detection of alkaline phosphatase-labeled molecules.
5-Bromo-4-chloro-3-indolyl phosphate disodium salt has been used to detect protein-antibody complexes. It has also been used for tissue staining in alkaline phosphatase histochemistry.
Legal Information
BCIP is a registered trademark of Merck KGaA, Darmstadt, Germany
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Este artículo | |||
|---|---|---|---|
| assay ≥98% (HPLC) | assay ≥95.0% (HPLC) | assay ≥99% | assay ≥94% (HPLC) |
| solubility H2O: 20 mg/mL, DMF: insoluble | solubility - | solubility DMF: 20 mg/mL, H2O: insoluble | solubility water: 5 mg/mL, clear to slightly hazy |
| Quality Level 200 | Quality Level 200 | Quality Level 200 | Quality Level 200 |
| form powder | form powder | form powder | form crystalline |
| storage temp. −20°C | storage temp. 2-8°C | storage temp. −20°C | storage temp. −20°C |
Clase de almacenamiento
11 - Combustible Solids
wgk
WGK 3
flash_point_f
Not applicable
flash_point_c
Not applicable
ppe
Eyeshields, Gloves, type N95 (US)
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Artículos
NBT-BCIP substrate system aids in western blotting and immunohistological staining, producing a blue-purple insoluble end product.
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Número de artículo de comercio global
| SKU | GTIN |
|---|---|
| B6149-1G | 04061833434475 |
| B6149-500MG | 04061835553921 |
| B6149-50MG | 04061835517688 |
| B6149-25MG | 04061833434482 |
| B6149-100MG | 04061833434468 |
| B6149-5G | 04061833434499 |