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W4502

Agua

suitable for electrophoresis, PCR

Water

Sinónimos:

Agua sin ADNasas, Agua sin ARNasas, Agua sin nucelasas, Agua sin proteasas, No tratada con DEPC

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A ustedes/SKUDisponibilidadPrecio
1 L

Envío estimado HOYdesdeMILWAUKEE

$86.00
10 x 50 mL

Envío estimado HOYdesdeMILWAUKEE

$151.00
6 x 1 L
Comprobar disponibilidad del carrito
$404.00
10 L

Envío estimado HOYdesdeMILWAUKEE

$421.00

Acerca de este artículo

Fórmula lineal:
H2O
Número CAS:
Peso molecular:
18.02
NACRES:
NA.31
PubChem Substance ID:
UNSPSC Code:
12191602
EC Number:
231-791-2
MDL number:
Beilstein/REAXYS Number:
2050024
Technique(s):
PCR: suitable, electrophoresis: suitable
Bp:
100 °C (lit.)
Vapor pressure:
3 mmHg

$86.00


Envío estimado HOYDetalles


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Nombre del producto

Agua, Nuclease-Free Water, for Molecular Biology

vapor density

<1 (vs air)

Quality Segment

vapor pressure

3 mmHg

sterility

0.1 μm filtered

form

liquid

technique(s)

PCR: suitable, electrophoresis: suitable

refractive index

n20/D 1.34 (lit.)

pH

5-7

bp

100 °C (lit.)

mp

0 °C (lit.)

density

1.000 g/mL at 3.98 °C (lit.)

foreign activity

DNase, RNase, Protease, free

SMILES string

O

InChI

1S/H2O/h1H2

InChI key

XLYOFNOQVPJJNP-UHFFFAOYSA-N

Application

Se ha utilizado agua-
  • en la síntesis de ADNc y la amplificación mediante PCR
  • para la dilución de una muestra de ADN ruminal antes de la prueba de PCR cuantitativa
  • en PCR (reacción en cadena de la polimerasa) y electroforesis en gel de agarosa
  • para la elución del ARN durante el aislamiento de ARN

Features and Benefits

  • Agua sin nucleasas
  • Sin ADNasas, sin ARNasas, sin proteasas
  • No tratada con dietilpirocarbonato (DEPC)
  • Filtrada en filtro de 0,1 µm
  • Preparada a partir de agua doblemente destilada y desionizada
  • Idónea para aplicaciones moleculares

Este producto se ha filtrado por 0,1 µm Se ha designado como Reactivo para biología molecular y es adecuada para aplicaciones de biología molecular. Se ha analizado la ausencia de nucleasas y proteasas en este producto, y se ha realizado un análisis del grado de contaminación microbiológica. No se ha tratado con dietilpirocarbonato (DEPC).

Preparation Note

Este producto se prepara a partir de agua desionizada y con doble destilación.

Other Notes

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Este artículo
9801-OP9528495289
technique(s)

PCR: suitable, electrophoresis: suitable

technique(s)

-

technique(s)

FISH: suitable

technique(s)

-

Quality Level

200

Quality Level

100

Quality Level

100

Quality Level

100

form

liquid

form

liquid

form

liquid

form

liquid

pH

5-7

pH

-

pH

5-7

pH

5-7

density

1.000 g/mL at 3.98 °C (lit.)

density

1.000 g/mL at 3.98 °C (lit.)

density

1.000 g/mL at 3.98 °C (lit.)

density

1.000 g/mL at 3.98 °C (lit.)

bp

100 °C (lit.)

bp

100 °C (lit.)

bp

100 °C (lit.)

bp

100 °C (lit.)


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Clase de almacenamiento

12 - Non Combustible Liquids

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves



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Questions

1–5 of 5 Questions  
  1. W4502 Millipore Water: what is the ohms of this water?

    1 answer
    1. The conductivity of this water is not determined. Currently, there is no Molecular Biology grade water product available that is tested for conductivity.

      Helpful?

  2. Do you have stability data for W4502 for evaluating if Shelf Life can be extended?

    1 answer
    1. This water product is not assigned an expiration or retest date. In the original sealed container the material is stable nearly indefinitely. Items that are not assigned an expiration date are warranteed for 1 year from the date of shipment.

      Helpful?

  3. Is this water sterile? Can this be used for cell culture?

    1 answer
    1. Product W4502 is not sterile, this product is prepared from Double Distilled and Deionized Water. This product is best suitable to use in molecular biology applications. See product W3500 for Sterile, Tissue Culture grade Water:
      https://www.sigmaaldrich.com/product/sigma/w3500

      Helpful?

  4. In what vessel does this product come? A bottle, a bag with a spigot, etc.?

    1 answer
    1. This product is packaged in square PET square bottles. To request a quote for larger volumes or specialized packaging, please submit a request at the link below:
      https://www.sigmaaldrich.com/services/support/bulk-quotation-request

      Helpful?

  5. This product does not list an expiration date on the label. Could someone provide an expiration for lot number BCCH7145?

    1 answer
    1. This product does not contain an expiration date or recommended retest date. Products with no expiration date or recommended retest date should be routinely inspected by customers to ensure they perform as expected. These products are also subject to a one year warranty from the date of shipment. For more information you may access the "Product Dating Information" document under "ADDITIONAL USEFUL DOCUMENTS ABOUT OUR PRODUCTS" at the bottom of the Quality Services page with this link: https://www.sigmaaldrich.com/life-science/quality-and-regulatory-management/quality-services

      Helpful?

Reviews

Active Filters

  1. USA
    • Review 1
    • Votes 6
    1 out of 5 stars.

    This water has microbial contamination!

    We used this water (Lot #0000387784) to dilute DNA for cell culture, but the cells kept getting contaminated. Eventually we traced it back to the water. Even a freshly opened bottle had microbial growth after mixing with culture medium. This ended up ruining thousands of dollars of experiments.

    Helpful?

    1. Response from MilliporeSigma:

      Thank you for your review. We are sorry to hear that your experience did not match your product expectations. We encourage customers who experience problems with our products to contact us for additional technical support. Please visit https://www.sigmaaldrich.com/US/en/support/customer-support to submit a Product Technical Inquiry.

  2. 1 Ratings-Only Review