β-Lactamase from Enterobacter cloacae

Name: β-Lactamase from <I>Enterobacter cloacae</I>
Brand: SIGMA

Type III, lyophilized powder, 6-18 units/mg protein (using benzylpenicillin)

Sinónimos:

β-Lactamase I, β-Lactamase II, Cephalosporinase, Penicillin amido-β-lactam hydrolase, Penicillinase from Enterobacter cloacae

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Número CAS:

9073-60-3

UNSPSC Code:

12352204

NACRES:

NA.54

Número CE:

3.5.2.6 (BRENDA, IUBMB)

MDL number:

MFCD00131819

Specific activity:

6-18 units/mg protein (using benzylpenicillin)

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Propiedades

type

Type III

form

lyophilized powder

specific activity

6-18 units/mg protein (using benzylpenicillin)

composition

Protein, ~10%

storage temp.

2-8°C

Descripción

Application

β--lactamase is used to inactivate β-lactam antibiotics by breaking open the β-lactam ring. β--lactamase is used to study antibiotic resistance and resistance suppression[1]. Product P4399 is produced from Enterobacter cloacae.

Biochem/physiol Actions

β--lactamase inactivates β-lactam antibiotics by breaking open the β-lactam ring[1].

Physical form

Lyophilized powder containing sodium phosphate and sodium citrate buffer salts

Preparation Note

Chromatographically purified

Analysis Note

Protein determined by biuret

Other Notes

One unit will hydrolyze 1.0 μmole of benzylpenicillin per min at pH 7.0 at 25 °C. This International Unit (using benzylpenicillin as substrate) is approximately equal to 600 Levy or 75 Pollock units.