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P1034

Phenyl isothiocyanate

Sigma Grade, 8.36 M, suitable for solid phase protein sequencing analysis, ≥99% (GC), liquid

Sinónimos:

PITC

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Fórmula lineal:
C6H5NCS
Número CAS:
103-72-0
Peso molecular:
135.19
UNSPSC Code:
12352118
NACRES:
NA.31
PubChem Substance ID:
24898144
EC Number:
203-138-1
Beilstein/REAXYS Number:
471392
MDL number:
MFCD00004798
Assay:
≥99% (GC)
Form:
liquid
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grade

Sigma Grade

Quality Segment

100

assay

≥99% (GC)

form

liquid

concentration

8.36 M

refractive index

n20/D 1.6515 (lit.)

bp

218 °C (lit.)

mp

−21 °C (lit.)

density

1.132 g/mL at 20 °C (lit.)

suitability

suitable for solid phase protein sequencing analysis

storage temp.

2-8°C

SMILES string

S=C=Nc1ccccc1

InChI

1S/C7H5NS/c9-6-8-7-4-2-1-3-5-7/h1-5H

InChI key

QKFJKGMPGYROCL-UHFFFAOYSA-N

General description

Phenyl isothiocyanate belongs to the class of aromatic compounds with isothiocyanate groups. It is typically used as a precolumn derivatization agent for the formation of phenylthiocarbamyl amino acids for their subsequent determination using chromatography techniques.

Application

Derivatizing reagent for primary and secondary amines. Used in sequencing peptides by Edman degradation and in amino acid analyses by HPLC.
Phenyl isothiocyanate may be used in the polypeptide and protein[1] sequencing at the subpicomole and subnanomole levels, respectively, by applying automated Edman chemistry followed by the analysis using high-performance liquid chromatography (HPLC) technique.

Packaging

One mL size packaged in flame sealed amber ampules under argon.
Ten mL size packaged in screw cap amber vials under argon.

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Este artículo
78780139742317861
Phenyl isothiocyanate Sigma Grade, 8.36 M, suitable for solid phase protein sequencing analysis, ≥99% (GC), liquid

Sigma-Aldrich

P1034

Phenyl isothiocyanate

Phenyl isothiocyanate for HPLC derivatization, the detection of alcohols and amines, ≥99.0%

Sigma-Aldrich

78780

Phenyl isothiocyanate

Phenyl isothiocyanate reagent grade, 98%

Sigma-Aldrich

139742

Phenyl isothiocyanate

Phenyl isothiocyanate 99%, for protein sequencing

Sigma-Aldrich

317861

Phenyl isothiocyanate

assay

≥99% (GC)

assay

≥99.0%

assay

98%

assay

99%

Quality Level

100

Quality Level

200

Quality Level

200

Quality Level

100

refractive index

n20/D 1.6515 (lit.)

refractive index

n20/D 1.649, n20/D 1.6515 (lit.)

refractive index

n20/D 1.6515 (lit.)

refractive index

n20/D 1.6515 (lit.)

storage temp.

2-8°C

storage temp.

2-8°C

storage temp.

-

storage temp.

2-8°C

bp

218 °C (lit.)

bp

218 °C (lit.)

bp

218 °C (lit.)

bp

218 °C (lit.), 221 °C

density

1.132 g/mL at 20 °C (lit.)

density

1.13 g/mL at 20 °C, 1.132 g/mL at 20 °C (lit.)

density

1.132 g/mL at 20 °C (lit.)

density

1.132 g/mL at 20 °C (lit.)

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Danger

Hazard Classifications

Acute Tox. 3 Oral - Resp. Sens. 1 - Skin Corr. 1B - Skin Sens. 1

Clase de almacenamiento

6.1A - Combustible acute toxic Cat. 1 and 2 / very toxic hazardous materials

wgk

WGK 3

flash_point_f

190.4 °F - closed cup

flash_point_c

88 °C - closed cup

ppe

Faceshields, Gloves, Goggles, type ABEK (EN14387) respirator filter

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Certificados de análisis (COA)

Lot/Batch Number
SDBB7158
STBL3619
STBL0610
STBJ8892
STBJ6409

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Visite la Librería de documentos

R L Heinrikson et al.
Analytical biochemistry, 136(1), 65-74 (1984-01-01)
Methods for the quantitative derivatization of amino acids with phenylisothiocyanate and for the separation and quantitation of the resulting phenylthiocarbamyl derivatives by reverse-phase high-performance liquid chromatography are described. Phenylthiocarbamylation of amino acids proceeds smoothly in 5 to 10 min at
Examination of automated polypeptide sequencing using standard phenyl isothiocyanate reagent and subpicomole high-performance liquid chromatographic analysis
Tempst P and Riviere L
Analytical Biochemistry, 183(2), 290-300 (1989)
The Protein Protocols Handbook, 235(1), 48-62 (1996)
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SKUGTIN
P1034-1ML04061838174888
P1034-10X1ML04061834353874
P1034-10ML04061834353867

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