This product has not been validated for deparaffinization of formalin-fixed, paraffin-embedded samples. However, an article in "Applied Immunohistochemistry & Molecular Morphology" (2014 Sep;22(8):623-6) indicated that mineral oil appears to work well for deparaffinizing these samples and for DNA extraction.
M8410
Aceite de inmersión
light oil, (neat)
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| Tamaño de envase | SKU | Disponibilidad | Precio |
|---|---|---|---|
| 5 mL | Disponible para enviar HOYdesdeMILWAUKEE | $20.90 | |
| 100 mL | Disponible para enviar HOYdesdeMILWAUKEE | $41.60 | |
| 500 mL | Disponible para enviar HOYdesdeMILWAUKEE | $68.30 | |
| 1 L | Disponible para enviar HOYdesdeMILWAUKEE | $116.00 |
Acerca de este artículo
$20.90
Disponible para enviar HOYDetalles
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Servicio técnico
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Permítanos ayudarleQuality Segment
sterility
non-sterile
form
light oil
storage condition
protect from light
refractive index
n20/D 1.467 (lit.)
density
0.84 g/mL at 25 °C (lit.)
format
neat
storage temp.
room temp
InChI key
AEOVEGJBKQQFOP-DDVLFWKVSA-L
Application
Se ha utilizado aceite mineral como recubrimiento en cultivos embrionarios en microgotas.
Analysis Note
Este material se ha analizado como recubrimiento en cultivos embrionarios en microgotas. (Todos los materiales analizados se prueban utilizando embriones de ratón.) Se utilizan normalmente los híbridos BCF1 x BDF1 El aceite se utiliza fundamentalmente para evitar la evaporación del medio, en particular cuando se realizan manipulaciones en el embrión.1 El procedimiento es el siguiente: Embriones de una célula (BCF1 x BDF1) se cultivaron en microgotas por triplicado del medio probado en embriones HTF (líquido tubárico humano) complementado con BSA al 0,4%. Para un análisis válido, al menos el 70% de los embriones de control en estadio de una célula deben desarrollarse al estadio de blastocisto en 96 horas. Para la liberación del producto, al menos el 80% de los embriones problema en estadio de una célula deben desarrollarse al estadio de blastocisto en 96 horas con el material analizado.
Disclaimer
Este producto no es estéril. El aceite mineral es difícil de esterilizar. Sólo puede ser esterilizado mediante filtración cuidadosa del aceite calentado. Se supone que se añadirán antibióticos al medio de cultivo. De lo contrario deberá utilizarse el producto de referencia M5310, que está esterilizado por filtración.
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Este artículo | |||
|---|---|---|---|
| Quality Level 200 | Quality Level 200 | Quality Level 300 | Quality Level 200 |
| form light oil | form light oil | form light oil | form viscous liquid |
| density 0.84 g/mL at 25 °C (lit.) | density 0.84 g/mL at 25 °C (lit.) | density 0.82-0.88 g/mL at 25 °C | density 0.833 g/mL at 25 °C |
| refractive index n20/D 1.467 (lit.) | refractive index n20/D 1.467 (lit.) | refractive index n20/D 1.467 (lit.) | refractive index n20/D 1.467 (lit.) |
| storage condition protect from light | storage condition - | storage condition protect from light | storage condition - |
| format neat | format - | format neat | format - |
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Clase de almacenamiento
10 - Combustible liquids
wgk
WGK 1
flash_point_f
No data available
flash_point_c
No data available
ppe
Eyeshields, Gloves
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Artículos
Mouse embryo media and embryo validated reagents for transgenic mouse embryo culture
TIE: A Method to Electroporate Long DNA Templates into Preimplantation Embryos for CRISPR-Cas9 Gene Editing
Bagheri H, et al.
The CRISPR journal, 1(3), 223-229 (2018)
Minoru Kubo et al.
Nucleic acids research, 47(9), 4539-4553 (2019-03-16)
Next-generation sequencing technologies have made it possible to carry out transcriptome analysis at the single-cell level. Single-cell RNA-sequencing (scRNA-seq) data provide insights into cellular dynamics, including intercellular heterogeneity as well as inter- and intra-cellular fluctuations in gene expression that cannot
Insulin and branched-chain amino acid depletion during mouse preimplantation embryo culture programmes body weight gain and raised blood pressure during early postnatal life
Velazquez M
Biochimica et Biophysica Acta (BBA)-Molecular Basis of Disease, 1864(2), 590-600 (2018)
Número de artículo de comercio global
| SKU | GTIN |
|---|---|
| M8410-1L | 04061838259844 |
| M8410-5ML | 04061834063070 |
| M8410-100ML | 04061838259837 |
| M8410-500ML | 04061838259851 |
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Can M8410 Sigma-Aldrich Mineral oil be used for the deparaffinization of FFPE samples, and is it compatible with downstream histological or molecular analyses like immunohistochemistry or RNA/DNA extraction?
1 answer-
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Hello,I´m looking for a mineral oil for sulfur pattern production. It´s difficult to find an oil in which the content of sulfur compounds appears in the COA. Can you inform if there´s a possibility of sulfur contamination in the production of mineral oil?
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The manufacturing process for this product is considered proprietary. Currently, there is no product option available that is tested for the presence of sulfur.
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ıs this suitable for preparation of carbon paste electrode?
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This material has not been validated in the process of making carbon paste electrodes. However, if the specifications (found below) meet your requirements, then there is no reason that they can't be used in this process.
https://www.sigmaaldrich.com/specification-sheets/990/164/M8410-BULK.pdfHelpful?
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Hello, Does this product have an odour? I want to use it for an olfactory memory assay, so needs to be odourless
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According to Section 9 of the Safety Data Sheet, this is an odorless chemical.
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What is the composition of the oil? Where does it comes from?
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M8410 is a light oil. Mineral oil is a complex mixture of different hydrocarbons and its typical composition is as follows:
Paraffinic oils, based on n-alkanes, 15-60%
Naphthenic oils, based on cycloalkanes, 30%-60%
Aromatic oils, based on aromatic hydrocarbons, 3-30%
Light and heavy mineral oil differ in terms of composition, viscosity, and density.
Mineral oil with an alkyl chain carbon number of 15 to 25 is categorized as light oil whereas 25 to 50 as heavy oil.
Light oil has a density of 0.83–0.86 kg L-1 whereas heavy oil has 0.875–0.905 kg L-1
Light mineral oil is less occlusive than heavy mineral oil as it contains more cyclic (saturated) moleculesAdditional Links: https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/product/documents/326/294/m8410pis.pdf and a second document: https://www.sigmaaldrich.com/specification-sheets/990/164/M8410-BULK.pdf
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