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M0643

Medio esencial mínimo Eagle

With Earle′s salts, L-glutamine, and non-essential amino acids, without sodium bicarbonate, powder, suitable for cell culture

Sinónimos:

MEM

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Tamaño de envaseSKUDisponibilidadPrecio
10 L

Disponible para enviar HOYdesdeMILWAUKEE

$49.40
10 x 1 L

Disponible para enviar HOYdesdeMILWAUKEE

$74.30
50 L

Disponible para enviar HOYdesdeMILWAUKEE

$153.00

Acerca de este artículo

NACRES:
NA.75
UNSPSC Code:
12352207

$49.40

Disponible para enviar HOYDetalles

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Quality Segment

500

form

powder

technique(s)

cell culture | mammalian: suitable

components

HEPES: no
Earle’s salts (5% CO2): yes
NaHCO3: no
phenol red: yes
L-glutamine: yes
sodium pyruvate: no

shipped in

ambient

storage temp.

2-8°C

General description

It contains 19 amino acids. The essential amino acids and the non-essential amino acids; L-ala; L-asn; L-asp; L-glu; L-gly; L-pro and L-ser. May be preferred for growth of adherent cells in the presence of reduced serum or as a base for development of a serum free medium.
Minimum Essential Medium Eagle consists of inorganic salts, water-soluble vitamins, sugar, and essential amino acids.[1] It is widely used as a media formulation for many cell culture studies.[2] It is a modified version of Basal medium Eagle (BME) and the concentrations of the majority of amino acids are twice as compared to BME.[1]

Application

Minimum Essential Medium Eagle has been used to culture osteoblast-like cell line MG-63, dispersed ventricular cells[3], and endometrial adenocarcinoma Ishikawa cells.[4]

Preparation Note

Formulated to contain 9.6 grams of powder per liter of medium.
Supplement with 2.2 g/L sodium bicarbonate.

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Este artículo
M7278M3024M4526
Sigma-Aldrich

Sigma-Aldrich

M0643

Minimum Essential Medium Eagle

Minimum Essential Medium Eagle HEPES Modification, with Earle′s salts, 25 mM HEPES and sodium bicarbonate, without L-glutamine, liquid, sterile-filtered, suitable for cell culture

Sigma-Aldrich

M7278

Minimum Essential Medium Eagle

Sigma-Aldrich

Sigma-Aldrich

M3024

Minimum Essential Medium Eagle

Minimum Essential Medium Eagle Alpha Modification, with sodium bicarbonate, without L-glutamine, ribonucleosides and deoxyribonucleosides, liquid, sterile-filtered, suitable for cell culture

Sigma-Aldrich

M4526

Minimum Essential Medium Eagle

technique(s)

cell culture | mammalian: suitable

technique(s)

cell culture | mammalian: suitable

technique(s)

cell culture | mammalian: suitable

technique(s)

cell culture | mammalian: suitable

form

powder

form

liquid

form

powder

form

liquid

components

HEPES: no
Earle’s salts (5% CO2): yes
NaHCO3: no
phenol red: yes
L-glutamine: yes
sodium pyruvate: no

components

L-glutamine: no
phenol red: yes
HEPES: 25 mM
sodium pyruvate: no
Earle’s salts (5% CO2): yes
NaHCO3: yes

components

Earle’s salts (5% CO2): yes
L-glutamine: no
HEPES: no
NaHCO3: no
sodium pyruvate: no
phenol red: no

components

Earle’s salts (5% CO2): yes
sodium pyruvate: yes
glucose: yes
L-glutamine: no
HEPES: no
Hanks’ salts (2% CO2): no
phenol red: yes
NaHCO3: yes
stable glutamine: no

storage temp.

2-8°C

storage temp.

2-8°C

storage temp.

2-8°C

storage temp.

2-8°C

shipped in

ambient

shipped in

ambient

shipped in

ambient

shipped in

ambient

Quality Level

500

Quality Level

400

Quality Level

500

Quality Level

400

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Clase de almacenamiento

11 - Combustible Solids

wgk

WGK 2

flash_point_f

Not applicable

flash_point_c

Not applicable

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Certificados de análisis (COA)

Lot/Batch Number
0000500472
0000500472
0000500470
0000500470
25D291

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Protocolos

Powdered Media Preparation Instructions

Powdered media and salt mixtures are extremely hygroscopic and should be protected from atmospheric moisture.

Artículos

MEM (Minimum Essential Medium Eagle)

A large selection of MEM formulations. Minimum Essential Medium (MEM), developed by Harry Eagle, is one of the most widely used of all synthetic cell culture media.

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Minimum Essential Medium Eagle (MEM) Specifications
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Journal of alloys and compounds, 161282-161282 (2021)
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European journal of pharmacology, 487(1-3), 29-36 (2004-03-23)
Concentration-dependent (10-1000 microM) effects of terpenoid phenol derivatives were studied on L-type Ca(2+) current in isolated canine and human ventricular cardiomyocytes using the whole-cell configuration of patch clamp technique. Carvacrol, thymol and eugenol suppressed peak Ca(2+) current at +5 mV
Tatsuma Yao et al.
Reproductive medicine and biology, 16(2), 99-117 (2017-12-21)
Cell culture technology has spread prolifically within a century, a variety of culture media has been designed. This review goes through the history, characteristics and current issues of animal-cell culture media. A literature search was performed on PubMed and Google
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Número de artículo de comercio global

SKUGTIN
M0643-50K04061834181491
M0643-10L04061833993750
M0643-1L04061837459504
M0643-100L04061837459498
M0643-50L04061833993767
M0643-10X1L04061835568802

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