L6533
Lectin from Dolichos biflorus (horse gram)
biotin conjugate, buffered aqueous solution
Sinónimos:
Dolichos biflorus agglutinin, DBA
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| Tamaño de envase | SKU | Disponibilidad | Precio |
|---|---|---|---|
| 5 mg | Comprobar disponibilidad del carrito | $557.00 |
Acerca de este artículo
NACRES:
NA.32
UNSPSC Code:
12352202
$557.00
Comprobar disponibilidad del carrito
Servicio técnico
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Permítanos ayudarleconjugate
biotin conjugate
Quality Level
form
buffered aqueous solution
composition
Protein, ≥2.5 mg/mL biuret
extent of labeling
≥3 mol biotin per mol lectin
storage temp.
−20°C
General description
Lectin from Dolichos biflorus (horse gram) is a heterotetrameric glycoprotein with a molecular mass of around 113 kDa. Dolichos biflorus agglutinin (DBA) is specific for terminal α-linked N-acetyl-D-galactosamine (GalNAc). It is a dietary lectin.
Application
Lectin from Dolichos biflorus (horse gram) has been used:
- to characterize mouse uterine natural killer (uNK) cells
- in double staining with lectins to localize nuclear factor-κB (NF-κB) activation
- as a species-specific gonocyte marker for the determination of donor cell colonization
- to label undifferentiated spermatogonia/cat testicular tissue
Biochem/physiol Actions
Dolichos biflorus agglutinin (DBA) possesses binding activity for which it is used as a marker binucleate cells and can indicate the state of their differentiation. It serves as a label for primordial germ cells (PGCs), gonocytes, pre-spermatogonia and spermatogonia.
DBA has anti-A1 human blood group specificity, useful for distinguishing between A1 and A2 blood types. DBA has strong anti-Cad activity, and has an affinity for terminal N-acetyl-α-D-galactosaminyl residues. Conjugates are prepared from affinity purified lectin.
Packaging
Sold on the basis of mg active conjugate.
Physical form
contains 10 mM HEPES, 0.15 M NaCl, pH 7.5, 0.08% sodium azide, 0.1 mM Ca2+
1 of 1
Este artículo | |||
|---|---|---|---|
| Quality Level 200 | Quality Level 300 | Quality Level 200 | Quality Level 200 |
| form buffered aqueous solution | form lyophilized powder | form lyophilized powder | form lyophilized powder |
| storage temp. −20°C | storage temp. −20°C | storage temp. 2-8°C | storage temp. −20°C |
| extent of labeling ≥3 mol biotin per mol lectin | extent of labeling - | extent of labeling ~2 mol FITC per mol protein | extent of labeling 2-4 mol biotin per mol protein |
| composition Protein, ≥2.5 mg/mL biuret | composition - | composition Protein, ~50% Lowry | composition - |
| conjugate biotin conjugate | conjugate - | conjugate FITC conjugate | conjugate biotin conjugate |
Clase de almacenamiento
10 - Combustible liquids
wgk
WGK 3
flash_point_f
Not applicable
flash_point_c
Not applicable
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Bovine sertoli cells colonize and form tubules in murine hosts following transplantation and grafting procedures
Zhang Z, et al.
Journal of Andrology, 29(4), 418-430 (2008)
Tubular NF-kB and AP-1 activation in human proteinuric renal disease
Mezzano SA, et al.
Kidney International, 60(4), 1366-1377 (2001)
Spermatogonial stem cell organization in felid testis as revealed by Dolichos biflorus lectin
Escada-Rebelo S, et al.
Andrology, 4(6), 1159-1168 (2016)
Número de artículo de comercio global
| SKU | GTIN |
|---|---|
| L6533-5MG | 04061833263525 |