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MilliporeSigma

HT60

Sigma-Aldrich

Amyloid Stain, Congo Red

Sinónimos:

Puchtler’s Modification for Amyloid

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1 KIT
$305.00

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1 KIT
$305.00

About This Item

Código UNSPSC:
41116121
NACRES:
NA.47

$305.00


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caducidad

Expiry date on the label.

DIV

for in vitro diagnostic use

dilution

(for histology)

aplicaciones

hematology
histology

temp. de almacenamiento

room temp

Principio

Amyloid protein is detected in tissue with Congo red, a metachromatic stain. Staining is intensified by pretreatment of tissue with alkaline sodium chloride.

Solo componentes del kit

Referencia del producto
Descripción

  • Congo Red Solution (kit only) 500 mL

  • Sodium Chloride Solution, Alcoholic (kit only) 500 mL

  • Sodium Hydroxide Solution (kit only) 2 x 12

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Referencia del producto
Descripción
Precios

Palabra de señalización

Danger

Frases de peligro

Clasificaciones de peligro

Carc. 1B - Eye Irrit. 2 - Flam. Liq. 2 - Met. Corr. 1 - Skin Irrit. 2

Código de clase de almacenamiento

3 - Flammable liquids

Punto de inflamabilidad (°F)

57.2 °F - closed cup

Punto de inflamabilidad (°C)

14.0 °C - closed cup


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Protocolos

A stem cell culture protocol to generate 3D NSC models of Alzheimer’s disease using ReNcell human neural stem cell lines.

Questions

1–2 of 2 Questions  
  1. Are there any protocols/literature available that have adapted Congo Red staining for cell cultures?

    1 answer
    1. There is no published procedure for using the HT60 kit for cultured cells, and such usage is regarded as off-label. The Congo Red Amyloid kit was designed for tissue sections, but most histological procedures can be used on cultured adherent cells. Specific considerations are mentioned for the thickness of cells and the use of a polarized microscope. Congo Red staining has specific requirements for cells to be stated at a certain thickness. If the cells are within the 6-12 micron range, no major adjustments to the protocol are expected. However, if the cells are extremely small or large, this might be an issue. Additionally, it is advised to utilize a polarized microscope for Congo Red staining, as it can be somewhat nonspecific when using a bright field microscope or an inverted microscope. Not everything that stains red under a light microscope with Congo Red is necessarily amyloid. Apple green staining with polarized microscopy is considered specific for Congo Red. It's important to note that not everything that stains red with Congo Red can automatically be assumed to be amyloid. Adapting a bright field microscope to use the polarized filters necessary for polarized microscopy is possible, but it is uncertain if this is possible when using an inverted microscope.

      Due to possible size variation, it is recommended to stain a series of slides if possible. Starting with the times stated in the pdf file is advised, and the staining time may be varied depending upon the thickness or size of the cultured cells. Trying both shortened and extended times depending upon the results obtained is also recommended.

      Helpful?

  2. What kind of mounting media should be used - organic or aqueous?

    1 answer
    1. Most histology procedures will instruct to mount in either a synthetic or an aqueous mounting media. Simply rinse three times in ethanol after coming out of the Alkaline Congo Red working solution. The slides should then be cleared in xylene and mounted. Also, mounting in a synthetic mounting media, such as DPX or other similar type permanent mounting medias can be tried.

      Helpful?

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