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H7782

Hybridization solution

Sinónimos:

Hyb solution, Hybridization solution for in situ hybridization, Ready-to-use hybridization solution

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Tamaño de envaseSKUDisponibilidadPrecio
6 mL

Disponible para enviar HOYdesdeMILWAUKEE

$116.00

Acerca de este artículo

UNSPSC Code:
12161503
EC Number:
200-842-0
NACRES:
NA.32

$116.00


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form

liquid

Quality Segment

technique(s)

FISH: suitable

storage temp.

−20°C

General description

In situ hybridization assays detect a specific nucleic acid sequence of a gene at the cellular level. Hybridization solution is used to enhance hybrid formation between the DNA probe and the target mRNA on tissue sections. It may be used for reconstituting and/or diluting the probe for in situ hybridization experiments, or as a negative control (without probe).
Ready-to-use hybridization solution optimized for in situ hybridization (ISH) procedures.

Application

Hybridization solution has been used in:
  • fluorescent in situ hybridization (FISH) to perform probe annealing[1]
  • in situ hybridization (ISH) to dilute probe for the detection of amastigote forms of Leishmania infantum[2]
  • FISH analysis to hybridize probes


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Este artículo
H7140S4040H7033
technique(s)

FISH: suitable

technique(s)

-

technique(s)

Northern blotting: suitable, Southern blotting: suitable

technique(s)

-

Quality Level

200

Quality Level

100

Quality Level

100

Quality Level

200

form

liquid

form

liquid (2x concentrate)

form

-

form

solution

storage temp.

−20°C

storage temp.

−20°C

storage temp.

-

storage temp.

room temp


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pictograms

Health hazard

signalword

Danger

Hazard Classifications

Carc. 2 - Repr. 1B - STOT RE 2 Oral

target_organs

Blood

Clase de almacenamiento

6.1C - Combustible acute toxic Cat.3 / toxic compounds or compounds which causing chronic effects

wgk

WGK 2

flash_point_f

Not applicable

flash_point_c

Not applicable



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SKUGTIN
H7782-6ML04061833804742

Questions

1–4 of 4 Questions  
  1. Does this hybridization mix contain 50% formamide?

    1 answer
    1. This product contains formamide, with the exact composition being proprietary. The Safety Data Sheet indicates that the formamide content is greater than or equal to 50% and less than 70%.

      Helpful?

  2. Is this buffer appropriate for Fluorescent in situ hybridization using probes labeled with ATTO by nick translation?

    1 answer
    1. This product has not been tested for the application. So, there is no reference data available.

      Helpful?

  3. Is this buffer appropriate to use with all probe types? Will it work with labeled (DIG, DNP, Biotin, etc.) polynucleotide probes?

    1 answer
    1. The buffer is optimized for both DNA and RNA probes for in situ hybridization. It has been used with FITC and biotinylated probes.

      Helpful?

  4. What is the composition of the Hybridization solution ( H7782)?

    1 answer
    1. The specific formulation of this product is considered proprietary but includes dextran sulfate and formamide in a sodium citrate solution.

      Helpful?

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