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G3907

Glutathione−Agarose

set of 3 pre-packed columns (2.5 ml each), (1:1 suspension in a 0.5 M NaCl + 20% ethanol solution)

Sinónimos:

GSH-agarose, S-linked glutathione agarose

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Tamaño de envaseSKUDisponibilidadPrecio
1 set
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$624.00

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UNSPSC Code:
41106500
NACRES:
NA.56
MDL number:
Matrix:
cross-linked 4% beaded agarose

$624.00


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Quality Segment

form

(1:1 suspension in a 0.5 M NaCl + 20% ethanol solution)

analyte chemical class(es)

proteins (GST)

packaging

set of 3 pre-packed columns (2.5 ml each)

technique(s)

immunoprecipitation (IP): suitable, protein purification: suitable

matrix

cross-linked 4% beaded agarose

storage temp.

2-8°C

General description

The resin in these columns consists of glutathione attached through the sulfur to epoxy activated, 4% cross-linked beaded agarose resulting in a 12 atom spacer. [1]

Application

Affinity chromatography using glutathione-agarose permits rapid, mild, non-denaturing and highly selective purification of glutathione binding enzymes such as glutathione-S-transferase, glutathione peroxidase, and glyoxalase I.[2]
The product was used in the design, synthesis and biological evaluation of new tryptamine and tetrahydro-β-carboline-based selective inhibitors of CDK4.[3] It was also used to study Fascaplysin-inspired diindolyls as selective inhibitors of CDK4/cyclin D1.[4]
Affinity chromatography using glutathione-agarose permits rapid, mild, non-denaturing and highly selective purification of proteins containing glutathione binding sequences, such as Glutathione S-Transferase (GST), glutathione peroxidase and glyoxalase I.

Physical form

1:1 suspension in a 0.5 M NaCl + 20% ethanol solutionl

Other Notes

Suspension of Product No. G 4510

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Este artículo
G4510AGRLE-ROAGRMP-RO
matrix

cross-linked 4% beaded agarose

matrix

4% cross-linked beaded agarose

matrix

-

matrix

-

technique(s)

immunoprecipitation (IP): suitable, protein purification: suitable

technique(s)

immunoprecipitation (IP): suitable, protein purification: suitable

technique(s)

-

technique(s)

-

packaging

set of 3 pre-packed columns (2.5 ml each)

packaging

-

packaging

pkg of 100 g (11685660001), pkg of 500 g (11685678001)

packaging

pkg of 100 g (11388983001), pkg of 500 g (11388991001)

analyte chemical class(es)

proteins (GST)

analyte chemical class(es)

proteins (GST)

analyte chemical class(es)

-

analyte chemical class(es)

-

Quality Level

200

Quality Level

200

Quality Level

-

Quality Level

-

form

(1:1 suspension in a 0.5 M NaCl + 20% ethanol solution)

form

lyophilized powder

form

powder

form

powder


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Clase de almacenamiento

10 - Combustible liquids

flash_point_f

180.1 °F

flash_point_c

82.3 °C



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Contenido relacionado

Investigate in vitro protein-protein interactions with pull-down assays, utilizing affinity, GST pull-down, TAP, and co-immunoprecipitation methods.

Investigue las interacciones proteína-proteína in vitro con análisis pull-down, utilizando métodos de afinidad, pull-down con GST, TAP y co-inmunoprecipitación.


Paul R Jenkins et al.
Bioorganic & medicinal chemistry, 16(16), 7728-7739 (2008-07-25)
We present the design, synthesis and biological activity of a library of substituted (biphenylcarbonyl)-tryptamine and (biphenylcarbonyl)-tetrahydro-beta-carboline compounds related to the natural product fascaplysin, as novel inhibitors of CDK4/cyclin D1. We show all these molecules, prepared using the Suzuki-Miyaura reaction, being
Carine Aubry et al.
Organic & biomolecular chemistry, 4(5), 787-801 (2006-02-24)
We present the design, synthesis, and biological activity of three classes of tryptamine derivatives, which are non-planar analogues of the toxic anti-cancer agent fascaplysin. We show these compounds to be selective inhibitors of CDK4 over CDK2, the most active compound
F Toribio et al.
Journal of chromatography. B, Biomedical applications, 684(1-2), 77-97 (1996-09-20)
The different preparative techniques and related analytical methods used for purification of glutathione peroxidase, glutathione transferase and glutathione reductase, described in papers published in the last ten years, have been reviewed in this article. Among the different purification techniques, chromatography



Número de artículo de comercio global

SKUGTIN
G3907-1SET04061833630327

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