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D8900

Dulbecco′s Modified Eagle′s Medium/Nutrient Mixture F-12 Ham

With L-glutamine and 15 mM HEPES, without sodium bicarbonate, powder, suitable for cell culture

Sinónimos:

DMEM Hams F12, DME/F-12, 1:1 mixture

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Tamaño de envaseSKUDisponibilidadPrecio
10 L

Disponible para enviar HOYdesdeMILWAUKEE

$124.00
10 x 1 L

Disponible para enviar HOYdesdeMILWAUKEE

$132.00
50 L

Disponible para enviar HOYdesdeMILWAUKEE

$529.00

Acerca de este artículo

UNSPSC Code:
12352207
NACRES:
NA.71
MDL number:

$124.00


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Quality Segment

form

powder

technique(s)

cell culture | mammalian: suitable

components

sodium pyruvate: 0.055 g/L
HEPES: 15 mM
NaHCO3: no
L-glutamine: yes
glucose: 3.15 g/L
phenol red: yes

shipped in

ambient

storage temp.

2-8°C

Application

Dulbecco′s Modified Eagle′s Medium/Nutrient Mixture F-12 Ham is suitable for the growth of a wide range of cell types in low serum conditions. It has been used:

  • as a component of the culture medium for endometrial explants in vitro culture
  • to immerse chitosan-gelatin-glycerol phosphate hydrogels for cytotoxicity evaluation
  • to culture nucleus pulposus (NP) cells to culture human articular chondrocytes for evaluating the toxicity of baicalein

Preparation Note

Formulated to contain 15.6 grams of powder per liter of medium.
Supplement with 1.2 g/L sodium bicarbonate.

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Este artículo
D0547D2906D8437
form

powder

form

powder

form

powder

form

liquid

technique(s)

cell culture | mammalian: suitable

technique(s)

cell culture | mammalian: suitable

technique(s)

cell culture | mammalian: suitable

technique(s)

cell culture | mammalian: suitable

components

sodium pyruvate: 0.055 g/L
HEPES: 15 mM
NaHCO3: no
L-glutamine: yes
glucose: 3.15 g/L
phenol red: yes

components

NaHCO3: no
sodium pyruvate: 0.11 g/L
HEPES: no
phenol red: yes
glucose: 3.15 g/L
L-glutamine: yes

components

NaHCO3: no
L-glutamine: yes
phenol red: no
sodium pyruvate: 0.055 g/L
glucose: 3.15 g/L
HEPES: 15 mM

components

glucose: 3.15 g/L
HEPES: yes
L-glutamine: yes
phenol red: yes
sodium pyruvate: 0.055 g/L

storage temp.

2-8°C

storage temp.

2-8°C

storage temp.

2-8°C

storage temp.

2-8°C

shipped in

ambient

shipped in

ambient

shipped in

ambient

shipped in

ambient

Quality Level

500

Quality Level

500

Quality Level

500

Quality Level

400


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Clase de almacenamiento

11 - Combustible Solids

wgk

WGK 1



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Protocolos

Powdered media and salt mixtures are extremely hygroscopic and should be protected from atmospheric moisture.

Artículos

Nutrient Mixture F-12 Ham Formulation

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High-quality ultrapure water that is endotoxin-free is recommended for mammalian cell culture as it support cell viability and prevents contamination.


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Número de artículo de comercio global

SKUGTIN
D8900-50L04061835568376
D8900-10X1L04061833590089
D8900-10L04061833590072
D8900-50K04061832954301

Questions

  1. What is the molarity of glucose when reconstituted with water?

    1 answer
    1. Upon reconstitution, the molarity of glucose is 17.5 mM (3.15 g/L).

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