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D8130

Ácido 5,5′-ditiobis(2-nitrobenzoico)

≥98%, BioReagent, suitable for determination of sulfhydryl groups

Sinónimos:

DTNB, Disulfuro de 3-carboxi-4-nitrofenilo, Disulfuro de bis(3-carboxi-4-nitrofenilo), Reactivo de Ellman, Ácido 6,6′-dinitro-3,3′-ditiodibenzoico

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Tamaño de envaseSKUDisponibilidadPrecio
500 mg

Disponible para enviar HOYdesdeMILWAUKEE

$31.50
1 g

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$56.60
5 g

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$139.00
10 g

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$257.00
25 g

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$516.00
100 g

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$1,990.00
$1,691.50

Acerca de este artículo

Fórmula lineal:
[-SC6H3(NO2)CO2H]2
Número CAS:
Peso molecular:
396.35
UNSPSC Code:
12352106
NACRES:
NA.26
PubChem Substance ID:
EC Number:
200-714-4
Beilstein/REAXYS Number:
1896821
MDL number:
Assay:
≥98%
Form:
powder

$31.50


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product line

BioReagent

Quality Segment

assay

≥98%

form

powder

mp

240-245 °C (dec.) (lit.)

suitability

suitable for determination of sulfhydryl groups

SMILES string

OC(=O)c1cc(SSc2ccc(c(c2)C(O)=O)[N+]([O-])=O)ccc1[N+]([O-])=O

InChI

1S/C14H8N2O8S2/c17-13(18)9-5-7(1-3-11(9)15(21)22)25-26-8-2-4-12(16(23)24)10(6-8)14(19)20/h1-6H,(H,17,18)(H,19,20)

InChI key

KIUMMUBSPKGMOY-UHFFFAOYSA-N

General description

El ácido 5,5′-ditio-bis(2-nitrobenzoico) se denomina también DTNB o reactivo de Ellman.[1] Es un reactivo hidrosoluble que interacciona con los grupos tiol y puede utilizarse cómodamente a pH neutro.[2] El anión tiolato (RS-) reacciona con el reactivo de Ellman (DTNB2-) para producir el anión 2-nitro-5-tiobenzoato (TNB2-) y un disulfuro mixto (R-S-TNB-). El TNB2- tiene un elevado coeficiente de absorción molar, y absorbe a 412 nm. Esta absorción es independiente del pH, a un pH> 7,3.[3]

Application

El reactivo de Ellman puede utilizarse para el análisis de la acetilcolinesterasa y para marcar de manera específica los residuos de cisteína.′[2] Es un reactivo sensible para medir el contenido sulfhidrilo libre en proteínas, péptidos y tejidos.

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Este artículo
D218200322123158194
assay

≥98%

assay

99%

assay

≥98% (titration)

assay

96%

Quality Level

200

Quality Level

200

Quality Level

100

Quality Level

200

form

powder

form

powder

form

solid

form

solid

mp

240-245 °C (dec.) (lit.)

mp

240-245 °C (dec.) (lit.)

mp

-

mp

155-157 °C (lit.)

product line

BioReagent

product line

ReagentPlus®

product line

-

product line

-

suitability

suitable for determination of sulfhydryl groups

suitability

-

suitability

-

suitability

-


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Clase de almacenamiento

11 - Combustible Solids

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

dust mask type N95 (US), Eyeshields, Gloves



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Certificados de análisis (COA)

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Protocolos

To measure chloramphenicol acetyltransferase activity, this procedure uses DTNB and coenzyme A. The reaction of DTNB with the –SH group on CoA results in a colorimetric increase at 412 nm.

Contenido relacionado

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Product Information Sheet

Ver todo el contenido relacionado

Ellman's reagent: 5,5'-dithiobis(2-nitrobenzoic acid)--a reexamination.
P W Riddles et al.
Analytical biochemistry, 94(1), 75-81 (1979-04-01)
Reassessment of Ellman's reagent.
P W Riddles et al.
Methods in enzymology, 91, 49-60 (1983-01-01)
Anne Bertling et al.
Arteriosclerosis, thrombosis, and vascular biology, 32(8), 1979-1990 (2012-04-28)
Staphylococcus aureus can induce platelet aggregation. The rapidity and degree of this correlates with the severity of disseminated intravascular coagulation, and depends on platelet peptidoglycans. Surface-located thiol isomerases play an important role in platelet activation. The staphylococcal extracellular adherence protein



Número de artículo de comercio global

SKUGTIN
D8130-10G04061835544141
D8130-50MG04061833690161
D8130-4MG04061833690154
D8130-150MG04061833412954
D8130-100G04061835544134
D8130-500MG04061835544172
D8130-8MG04061833460979
D8130-1G04061835544158
D8130-5G04061835544189
D8130-25G04061835544165

Questions

1–4 of 4 Questions  
  1. How can I determine the shelf life / expiration / retest date of this product?

    1 answer
    1. If this product has an expiration or retest date, it will be shown on the Certificate of Analysis (COA, CofA). If there is no retest or expiration date listed on the product's COA, we do not have suitable stability data to determine a shelf life. For these products, the only date on the COA will be the release date; a retest, expiration, or use-by-date will not be displayed.
      For all products, we recommend handling per defined conditions as printed in our product literature and website product descriptions. We recommend that products should be routinely inspected by customers to ensure they perform as expected.
      For products without retest or expiration dates, our standard warranty of 1 year from the date of shipment is applicable.
      For more information, please refer to the Product Dating Information document: https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/marketing/global/documents/418/501/product-dating-information-06-25-mk.pdf

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  2. How is shipping temperature determined? And how is it related to the product storage temperature?

    1 answer
    1. Products may be shipped at a different temperature than the recommended long-term storage temperature. If the product quality is sensitive to short-term exposure to conditions other than the recommended long-term storage, it will be shipped on wet or dry-ice. If the product quality is NOT affected by short-term exposure to conditions other than the recommended long-term storage, it will be shipped at ambient temperature. As shipping routes are configured for minimum transit times, shipping at ambient temperature helps control shipping costs for our customers. For more information, please refer to the Storage and Transport Conditions document: https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/marketing/global/documents/316/622/storage-transport-conditions-mk.pdf

      Helpful?

  3. To dissolve DTNB which alcohol should use?

    1 answer
    1. This product is tested for solubility in Ethanol at 10 mg/mL. Solutions may also be prepared at 5mM in 100 mM sodium or potassium phosphate buffer, pH 7.2, containing 0.1 mM EDTA. Please see the link below to review the entire product datasheet for additional information:
      https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/product/documents/414/707/d8130pis.pdf

      Helpful?

  4. what is the storage temperature for this chemical

    1 answer
    1. This product can be stored at room temperature.

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