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D225

4,5-Diaminofluorescein diacetate solution

5 mM in DMSO, ≥97% (HPLC)

Sinónimos:

DAF-2 DA

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Tamaño de envaseSKUDisponibilidadPrecio
1 mg
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$865.00

Acerca de este artículo

UNSPSC Code:
41116100
NACRES:
NA.28
MDL number:

$865.00


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Quality Segment

assay

≥97% (HPLC)

form

solution

availability

not available in Japan

concentration

5 mM in DMSO

shipped in

wet ice

storage temp.

−20°C

General description

4,5-Diaminofluorescein diacetate (DAF-2 DA) solution is a membrane-permeable fluorescent probe, which measures nitric oxide (NO) in living cells. It is used to study the synthesis of NO in bovine aortic endothelial cells (BAECs). DAF-2 DA imaging shows that ciliated epithelial cells produces high amount of NO than non-epithelial inflammatory cells.

Application

Cell-permeable fluorescent detector of nitric oxide in living cells.

Legal Information

DAF-2 is manufactured by Daiichi Pure Chemicals Co., LTD.
US Patent No. 5,874,590

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Este artículo
D2813D224251505-M
concentration

5 mM in DMSO

concentration

-

concentration

5 mM in DMSO

concentration

-

form

solution

form

lyophilized powder

form

solution

form

solution

shipped in

wet ice

shipped in

wet ice

shipped in

-

shipped in

-

assay

≥97% (HPLC)

assay

≥98% (HPLC)

assay

≥97% (HPLC)

assay

≥98% (HPLC)

storage temp.

−20°C

storage temp.

2-8°C

storage temp.

2-8°C

storage temp.

−20°C

Quality Level

100

Quality Level

100

Quality Level

100

Quality Level

100


Clase de almacenamiento

10 - Combustible liquids

wgk

WGK 2

flash_point_f

188.6 °F - closed cup

flash_point_c

87 °C - closed cup



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Contenido relacionado

Nitric oxide (NO) as a signal transporter in neurons, endothelial cells and in the immune system.


Determinants of shear stress-stimulated endothelial nitric oxide production assessed in real-time by 4, 5-diaminofluorescein fluorescence
Qiu W, et al.
Biochemical and Biophysical Research Communications, 286(2), 328-335 (2001)
Increased nitric oxide production in nasal epithelial cells from allergic patients-RT-PCR analysis and direct imaging by a fluorescence indicator: DAF-2 DA
Takeno S, et al.
Clinical and Experimental Allergy, 31(6), 881-888 (2001)
Anne Marie W Bartosch et al.
Biophysical journal, 113(1), 101-108 (2017-07-13)
Nitric oxide (NO) is a regulatory molecule in the vascular system and its inhibition due to endothelial injury contributes to cardiovascular disease. The glycocalyx is a thin layer of glycolipids, glycoproteins, and proteoglycans on the surface of mammalian epithelial cells.



Número de artículo de comercio global

SKUGTIN
D225-1MG04061833562017

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