C8554

Chorionic gonadotropin human

lyophilized powder, suitable for mouse embryo cell culture

• Sinónimos: Choriogonin, HCG
• Número CAS: 9002-61-3
• UNSPSC Code: 12352202
• EC Number: 232-660-2
• NACRES: NA.32
• MDL number: MFCD00130797
• Biological source: human urine
• Form: lyophilized powder

Propiedades

• biological source: human urine
• Quality Segment: 200
• sterility: aseptically processed
• form: lyophilized powder
• potency: ≥5,000 I.U. per mg
• mol wt: 37.9 kDa, apparent mol wt 37.9 kDa by SDS-PAGE
• drug control: Home Office Schedule 4.2
• technique(s): cell culture | embryo: suitable
• impurities: HBV,HCV and HIV, tested negative
• solubility: H₂O: 1 mL/vial, saline: 1 mL/vial
• UniProt accession no.: P01215
• shipped in: dry ice
• storage temp.: −20°C
• Gene Information: human ... CGA(1081)

Descripción

General description

Glycoprotein hormone consisting of a 92-amino acid α-chain which is identical to that of LH, FSH, and TSH; and a distinct 149-amino acid β-chain. Produced by chorionic tissue and responsible for maintaining the corpus luteum during early pregnancy. Also produced by trophoblast cell neoplasms, which are marked by elevated free hCG β-chains and hyperglycosylated hCG.

Note: Actual molecular weight will vary from the theoretical depending upon the degree of glycosylation. Theoretical MW is 37.9 kDa based on native form which contains 2 subunits: α = 14.9 kDa (~10.2 kDa for the polypeptide and ~4.7 kDa for the carbohydrate), and β = 23.0 kDa (~16.0 kDa for the polypeptide and ~7.0 kDa for the carbohydrate). See J. Biol. Chem. 248, 6796-6827 (1973).

Application

Recommended for superovulation induction in mice.

Biochem/physiol Actions

Human chorionic gondadotropin (hCG) may be used as an alternative to PMSG to induce ovulation in laboratory animals.[1] When hCG was injected into laboratory animals via intravenous (i.v.), intraperitoneal (i.p.), or subcutaneous (s.c.) methodstet, the effective dose values for 50% of the animals (ED50) per animal and per body weight (kg) in parenthesis were as follows:
Mice: i.v. 0.2 (7.7) I.U.; i.p. 0.3 (11.5) I.U.; s.c. 0.7 (26.9) I.U.
Syrian hamsters: i.v. 1.0 (9.5) I.U.; i.p. 1.8 (17.1) I.U.; s.c. 2.6 (24.8) I.U.
Rats: i.v. 1.3 (4.6) I.U.; i.p. 3.5 (12.3) I.U.; s.c.7.5 (26.3) I.U.

Using PMSG, the ED50 values per animal and per body weight (kg) in parenthesis were as follows:
Mice: i.v. 0.8 (30.8) I.U.; i.p. 2.0 (76.9) I.U.; s.c. 2.8 (107.7) I.U
Syrian hamsters: i.v., 3.6 (34.3) I.U.; i. p., 8.0 (76.2) I.U.; s.c., 13.2 (125.7) I.U.
Rats: i.v., 6.0 (76.8) I.U.; i p., 20.8 (73.0) I.U.; s.c., 76.8 (269.5) I.U.

Información de seguridad

• pictograms: GHS08
• signalword: Warning
• hcodes: H361
• pcodes: P281
• Hazard Classifications: Repr. 2
• Clase de almacenamiento: 11 - Combustible Solids
• wgk: WGK 3
• flash_point_f: Not applicable
• flash_point_c: Not applicable