C6098

Monoclonal Anti-CCR-6 antibody produced in mouse

clone 53103.111, purified immunoglobulin, lyophilized powder

• UNSPSC Code: 51111800
• MDL number: MFCD01864609
• Conjugate: unconjugated
• Clone: 53103.111, monoclonal
• Application: FACS, IHC
• Citations: 4

Propiedades

• biological source: mouse
• conjugate: unconjugated
• antibody form: purified immunoglobulin
• antibody product type: primary antibodies
• clone: 53103.111, monoclonal
• form: lyophilized powder
• species reactivity: human
• technique(s): flow cytometry: suitable using human PBMCs (1 μg/10⁶ cells), immunohistochemistry: 25 μg/mL using frozen human tissue sections
• isotype: IgG2b
• UniProt accession no.: P51684
• storage temp.: −20°C
• Gene Information: human ... CCR6(1235) ; mouse ... Ccr6(12458)

Descripción

General description

Chemokine receptor CCR6 plays an important role in adaptive immunity, recruitment of CD4⁺ T cells and dendritic cells to sites of epithelial inflammation. It is expressed on leukocyte subsets of T cells, B cells, immature dendritic cells, neutrophils, monocytes and NKT cells. CCR6 binds the chemokine, CCL20 (macrophage inflammatory protein-3α) and induces proinflammatory signals such as TNF-α, IFN-γ and toll-like receptor agonists that in turn activate ERK1/2 and p38 MAPK pathways. CCR6 has been linked to autoimmune diseases of multiple sclerosis, rheumatoid arthritis, psoriasis and atherosclerosis. Monoclonal Anti-CCR-6 reacts with CCR-6 transfected cells and not with the parent cell line. The antibody shows no cross-reactivity with CCR-1, CCR-2, CCR-3, CCR-4, CCR-5, CCR-7, CXCR-1, CXCR-2, CXCR-3, CXCR-4 and STRL33 transfected cells.

Immunogen

human CCR-6 transfected NSO mouse myeloma cells.

Application

Monoclonal Anti-CCR-6 antibody may be used for flow cytometry at a working concentration of 1μg/10⁶ cells using human peripheral blood mononuclear cells. Antibody concentration of 25 μg/ml may be used for immunohistochemistry of frozen human tissue sections.

Biochem/physiol Actions

The antibody reacts with human CCR-6 transfectant cells and not the parent cell line. The antibody shows no cross-reactivity with CCR-1, CCR-2, CCR-3, CCR-4, CCR-5, CCR-7, CXCR-1, CXCR-2, CXCR-3, CXCR-4 or STRL33.

Physical form

Lyophilized from a 0.2 μm filtered solution in phosphate buffered saline with 5% trehalose.

Preparation Note

Purified using protein G.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Información de seguridad

• Clase de almacenamiento: 11 - Combustible Solids
• wgk: WGK 1
• flash_point_f: Not applicable
• flash_point_c: Not applicable