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B5144

Bromelain from pineapple stem

≥4 units/mg protein, (chromatography purified)

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Tamaño de envaseSKUDisponibilidadPrecio
100 units

Disponible para enviar HOYdesdeMILWAUKEE

$471.00
250 units

Disponible para enviar HOYdesdeMILWAUKEE

$945.00

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Número CAS:
UNSPSC Code:
12352204
NACRES:
NA.54
EC Number:
253-387-5
MDL number:
Número CE:
Specific activity:
(chromatography purified), ≥4 units/mg protein

$471.00


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form

lyophilized powder

Quality Level

specific activity

(chromatography purified), ≥4 units/mg protein

composition

Protein, 30-50% biuret

solubility

H2O: soluble 1.0 mg/mL, clear, colorless

storage temp.

−20°C

Application

Bromelain from pineapple stem has been used as a positive control in sodium dodecyl sulfate (SDS) / polyacrylamide gel electrophoresis (PAGE) zymograms. It has also been used as a Azocasein substrate for the determination of proteolytic activity.
Bromelain may be used to inhibit the biosysnthesis of proinflammatory prostaglandins. It may be used to reduce clotting efficiency. Bromelain, from pineapple stem, has been used to make enzymatic hydrolysates of honeybee-collected pollen .

Biochem/physiol Actions

Bromelain is a cysteine endopeptidase with broad specificity for cleavage of proteins. Bromelain may be from a stem or piece of fruit. Product P3169 (pGlu-Phe-Leu-p-nitroanilide) may be used as a chromogenic substrate for the assay of bromelain.

Physical form

Lyophilized powder containing mannitol and potassium phosphate buffer salts

Preparation Note

Chromatographically purified
Chromatographically purified. Dissolves in water at 1 mg/mL concentration to form a clear, colorless solution.

Other Notes

One unit will release 1.0 μmole of p-nitrophenol from N-α-Z-L-lysine p-nitrophenyl ester per min at pH 4.6 at 25 °C.

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1 of 1

Este artículo
B4882P6675C9658
specific activity

(chromatography purified), ≥4 units/mg protein

specific activity

≥3 units/mg protein

specific activity

≥100 units/mg protein

specific activity

≥3 units/mg protein

form

lyophilized powder

form

lyophilized powder

form

lyophilized powder

form

lyophilized powder

storage temp.

−20°C

storage temp.

−20°C

storage temp.

−20°C

storage temp.

−20°C

solubility

H2O: soluble 1.0 mg/mL, clear, colorless

solubility

-

solubility

-

solubility

H2O: soluble 0.5 mg/mL

Quality Level

200

Quality Level

200

Quality Level

200

Quality Level

300

composition

Protein, 30-50% biuret

composition

Protein, ≥30% biuret

composition

Protein, 20-74% Lowry

composition

Protein, ~70% biuret


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pictograms

Health hazardExclamation mark

signalword

Danger

Hazard Classifications

Eye Irrit. 2 - Resp. Sens. 1 - Skin Irrit. 2 - STOT SE 3

target_organs

Respiratory system

Clase de almacenamiento

13 - Non Combustible Solids

wgk

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable



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Azocasein substrate for determination of proteolytic activity: reexamining a traditional method using bromelain samples
Coelho DF, et al.
BioMed Research International (2016)
Acidic cysteine protease inhibitors from pineapple stem.
R L Heinrikson et al.
Methods in enzymology, 45, 740-751 (1976-01-01)
Ashley G Yow et al.
Scientific reports, 13(1), 11605-11605 (2023-07-19)
Papain (aka C1A) family proteases, including bromelain enzymes, are widespread across the plant kingdom and play critical regulatory functions in protein turnover during development. The proteolytic activity exhibited by papain family proteases has led to their increased usage for a



Número de artículo de comercio global

SKUGTIN
B5144-250UN04061833433591
B5144-100UN04061833433584

Questions

  1. Good day! I would like to ask if do you have a Bromelain Powder in CDU? We would like to purchase for our research regarding bromelain enzyme.

    1 answer
    1. Currently, there are no Bromelain products tested with casein and reported in CDU.

      Helpful?

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