If this product has an expiration or retest date, it will be shown on the Certificate of Analysis (COA, CofA). If there is no retest or expiration date listed on the product's COA, we do not have suitable stability data to determine a shelf life. For these products, the only date on the COA will be the release date; a retest, expiration, or use-by-date will not be displayed.
For all products, we recommend handling per defined conditions as printed in our product literature and website product descriptions. We recommend that products should be routinely inspected by customers to ensure they perform as expected.
For products without retest or expiration dates, our standard warranty of 1 year from the date of shipment is applicable.
For more information, please refer to the Product Dating Information document: https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/marketing/global/documents/418/501/product-dating-information-06-25-mk.pdf
77617
Fenol – cloroformo – mezcla de alcohol isoamílico
BioUltra, Molecular Biology, 25:24:1
Seleccione un Tamaño
| A ustedes/SKU | Disponibilidad | Precio |
|---|---|---|
100 mL | Disponible para enviar HOYdesdeMILWAUKEE | $139.00 |
500 mL | Disponible para enviar HOYdesdeMILWAUKEE | $443.00 |
Acerca de este artículo
grade
Molecular Biology
Quality Segment
product line
BioUltra
form
liquid
technique(s)
ChIP: suitable, DNA extraction: suitable, DNA purification: suitable
impurities
DNases, none detected, RNases, none detected, phosphatases, none detected, proteases, none detected
pH
7.2-8.3(H2O-phase, after extraction with H2O, 1:1)
density
1.28 g/mL at 20 °C
absorption
cut-off at 360 nm
suitability
suitable for molecular biology
application(s)
agriculture
storage temp.
2-8°C
SMILES string
ClC(Cl)Cl.CC(C)CCO.Oc1ccccc1
InChI
1S/C6H6O.C5H12O.CHCl3/c7-6-4-2-1-3-5-6;1-5(2)3-4-6;2-1(3)4/h1-5,7H;5-6H,3-4H2,1-2H3;1H
InChI key
ZYWFEOZQIUMEGL-UHFFFAOYSA-N
General description
Las aplicaciones de la mezcla PCI son diversas, y abarcan el aislamiento del ARN, donde extrae de manera eficaz el ARN de los tejidos animales y vegetales para análisis posteriores como la transcripción inversa y los estudios de expresión génica. Además, la mezcla PCI también ha demostrado ser fundamental en la extracción del ADN, lo que permite una recuperación eficiente para diversas aplicaciones, como las huellas genéticas ubicadas en los minisatélites del ADN para la identificación genética. Como técnica de purificación general, la mezcla PCI garantiza preparaciones de gran calidad tanto de ADN como de ARN para futuras investigaciones.
Application
Biochem/physiol Actions
Features and Benefits
- Versátil y adaptable para una amplia variedad de aplicaciones de laboratorio y experimentales
- Sin ADNasas, ARNasas, NICKasas y proteasas
Physical form
Other Notes
1 of 1
Este artículo | |||
|---|---|---|---|
| Quality Level 200 | Quality Level 100 | Quality Level 100 | Quality Level 200 |
| application(s) agriculture | application(s) - | application(s) - | application(s) - |
| grade Molecular Biology | grade Molecular Biology | grade Molecular Biology | grade Molecular Biology |
| technique(s) ChIP: suitable, DNA purification: suitable, DNA extraction: suitable | technique(s) - | technique(s) - | technique(s) GC/GC: suitable |
| form liquid | form liquid | form liquid | form liquid |
| impurities DNases, none detected, phosphatases, none detected, RNases, none detected, proteases, none detected | impurities DNases, none detected, RNases, none detected, phosphatases, none detected, proteases, none detected | impurities DNases, none detected, RNases, none detected, phosphatases, none detected, proteases, none detected | impurities DNases, none detected, RNases, none detected, insoluble matter, passes filter test, phosphatases, none detected, proteases, none detected, ≤0.001% free alkali (as NH3) |
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signalword
Danger
Hazard Classifications
Acute Tox. 3 Inhalation - Acute Tox. 3 Oral - Acute Tox. 4 Dermal - Aquatic Chronic 2 - Carc. 2 - Eye Dam. 1 - Muta. 2 - Repr. 2 - Skin Corr. 1B - STOT RE 1 Oral - STOT RE 2 - STOT SE 3
target_organs
Central nervous system, Liver,Kidney, Nervous system,Kidney,Liver,Skin
Clase de almacenamiento
6.1A - Combustible acute toxic Cat. 1 and 2 / very toxic hazardous materials
flash_point_f
174.2 °F
flash_point_c
79 °C
ppe
Faceshields, Gloves, Goggles, type ABEK (EN14387) respirator filter
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How can I determine the shelf life / expiration / retest date of this product?
1 answer-
Helpful?
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Hello, Is this product saturated with Tris-Hcl?
1 answer-
Yes, Tris HCl is used to prepare the 100 mM Tris pH 8.0 saturation buffer.
Helpful?
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Is the packaging configuration on the 100 ml presentation glass, amber bottle?
1 answer-
Yes, this product is packaged in an amber glass bottle.
Helpful?
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Dans la description, il est dit que le produit est "composé de 25 % de phénol, 24 % de chloroforme et 1 % d'alcool isoamylique". Les 50% restant, qu'est-ce que c'est ou est-ce une erreur ?
1 answer-
As reported in the 'Physical Form' section, under 'DESCRIPTION', the remainder of this material is 100 mM TRIS, pH 8.0, containing approximately 0.1% 8-hydroxyquinoline.
Helpful?
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How is shipping temperature determined? And how is it related to the product storage temperature?
1 answer-
Products may be shipped at a different temperature than the recommended long-term storage temperature. If the product quality is sensitive to short-term exposure to conditions other than the recommended long-term storage, it will be shipped on wet or dry-ice. If the product quality is NOT affected by short-term exposure to conditions other than the recommended long-term storage, it will be shipped at ambient temperature. As shipping routes are configured for minimum transit times, shipping at ambient temperature helps control shipping costs for our customers. For more information, please refer to the Storage and Transport Conditions document: https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/marketing/global/documents/316/622/storage-transport-conditions-mk.pdf
Helpful?
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Is there an overlay layer of aqueous TE buffer in the bottles?
1 answer-
The product is saturated with TRIS-buffer. At the time of filling the product consists of a single phase. It is possible that an aqueous phase will be liberated, particularly at lower temperatures. Should phase separation occur, the upper will be the aqueous Tris.
Helpful?
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