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MilliporeSigma

75554

Millipore

Aminopeptidase Test Strips

suitable for agriculture, clinical testing, environmental, food and beverages, pharmaceutical, microbiology and specific enzyme detection

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About This Item

UNSPSC Code:
41171621
NACRES:
NA.85

product name

Aminopeptidase Test, suitable for microbiology

product line

BioChemika

shelf life

limited shelf life, expiry date on the label

technique(s)

microbe id | specific enzyme detection: suitable

antibiotic activity spectrum

Gram-negative bacteria

application(s)

agriculture
clinical testing
environmental
food and beverages
pharmaceutical

microbiology

storage temp.

2-8°C

suitability

bacteria

General description

Aminopeptidase Test is used for the detection of L-alanine-aminopeptidase in microorganisms. L-Alanine aminopeptidase is an enzyme localized in the bacterial cell wall which cleaves N-terminal L-alanine from various peptides. It is found almost exclusively in Gram-negative microorganisms. Studied Gram-positive or Gram-variable microorganisms show no or very weak activity. The aminopeptidase test is a reliable method for determining the Gram behavior and differentiating Gram-positive from Gram-negative bacteria. It does not replace Gram-staining, as it cannot show morphology.

Application

Aminopeptidase Test has been used to perform a Gram analysis of bacteria isolated from Scleroderma citrinum mycorrhizae, the mycorrhizosphere and bulk soil.
For the detection of L-alanine-aminopeptidase in microorganisms.
L-Alanine aminopeptidase is an enzyme localized in the bacterial cell wall which cleaves N-terminal L-alanine from various peptides. It is found almost exclusively in Gram-negative microorganisms. Studied Gram-positive or Gram-variable microorganisms show no or very weak activity. The aminopeptidase test is a reliable method for determining the Gram behavior. It does not replace Gram-staining, as it cannot show morphology.

Components

50 Test Strips (the reaction zone of each strip contains 0.5 μmol L-alanine-4-nitroanilide and buffering agents)

Storage Class

11 - Combustible Solids

wgk_germany

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, type N95 (US)


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S.Uroz
Applied and Environmental Microbiology, 73(9), 3019-3027 (2007)
J Fink-Gremmels
Animal Feed Contamination (2012)

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