Clinica chimica acta; international journal of clinical chemistry, 411(19-20), 1536-1538 (2010-06-24)
In clinical settings, human serum albumin (HSA) is widely quantitated on the basis of change in the color of bromocresol purple (BCP) bound to HSA. However, the binding sites of BCP on HSA are hitherto unknown. We have identified these
Applied radiation and isotopes : including data, instrumentation and methods for use in agriculture, industry and medicine, 69(2), 377-380 (2010-11-27)
Polyvinyl chloride (PVC) dyed with bromocresol purple was investigated as a high-dose radiation dosimeter. The absorbance at 417 nm depends linearly on the dose below 50 kGy. The response depends neither on dose rate nor on the irradiation temperature. The
Journal of photochemistry and photobiology. B, Biology, 90(1), 1-7 (2007-11-21)
The interaction between bromocresol purple (BCP) and bovine serum albumin (BSA)/porcine serum albumin (PSA) was investigated both in the absence and presence of bilirubin (BR) using absorption/absorption difference spectroscopy. A significant red shift in the absorption maxima of BCP accompanied
Journal of pharmaceutical and biomedical analysis, 40(1), 151-156 (2005-08-23)
A simple, extraction-free spectrophotometric method is proposed for the analysis of some beta-blockers, namely atenolol, timolol and nadolol. The method is based on the interaction of the drugs in chloroform with 0.1% chloroformic solutions of acidic sulphophthalein dyes to form
Microplate readers have been useful assistants of researchers for several decades. This work is focused on the applications of a simple absorbance microplate reader in yeast physiology research, and its advantages and limitations in comparison with alternative methods are discussed.
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