The following protocol is recommended:
1. Before opening the vial, spin down the solid to the bottom by a
microcentrifuge or by a desktop centrifuge.
2. Warm the vial to room temperature and add DMSO to prepare a
1000X stock solution of 1-5 mM (freeze aliquots at -20°C).
3. Dilute in cell culture media at a final concentration of 1-5 µM and add
to cells in culture. Incubate at 37°C for 20-45 minutes.
4. Wash cells with PBS buffer before imaging.
SCT047
BioTracker Cystine-FITC Live Cell Dye
Live cell imaging dye that measures cystine uptake at the single cell level used to detect glutathione (GSH) synthesis, reactive oxygen species (ROS) detoxification, T-cell activation and B-cell development.
Sinónimos:
Cellular cystine uptake detection, Live cell imaging probe
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NACRES:
NA.47
UNSPSC Code:
12352207
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Servicio técnico
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Permítanos ayudarleassay
≥98% (H-NMR), ≥98% (HPLC), ≥98% (LC/MS), ≥98% (elemental analysis)
technique(s)
cell based assay: suitable
detection method
fluorometric
shipped in
ambient
storage temp.
-10 to -25°C
General description
Uptake of cystine allows accumulation of cysteine that is necessary for glutathione synthesis and reactive oxygen species (ROS) detoxification. After the uptake of cystine into the cytoplasm, the reducing intracellular environment leads to a separation of one cystine molecule into two cysteine molecules. A key function of cysteine is to maintain the cellular pools of glutathione (GSH). Cystine uptake is also required for T-cell activation and B-cell development.
The BioTracker Cystine-FITC dye is a live cell green fluorescent imaging probe that measures cystine uptake at the single cell level. The probe has been used to measure T-cell activation using traditional and imaging flow cytometry methods.
Spectral Properties
Absorbance: 450nm
Emission: 525nm
The BioTracker Cystine-FITC dye is a live cell green fluorescent imaging probe that measures cystine uptake at the single cell level. The probe has been used to measure T-cell activation using traditional and imaging flow cytometry methods.
Spectral Properties
Absorbance: 450nm
Emission: 525nm
Application
Live cell fluorescent imaging
Research Category
Cell Imaging
Cell Imaging
Research Sub Category
Live Cell Dye
Live Cell Dye
Physical form
Lyophilized
Preparation Note
Store BioTracker Cystine-FITC Live Cell Dye at -20°C, desiccate and protect from light
Note: Centrifuge vial briefly to collect contents at bottom of vial before opening.
Note: Centrifuge vial briefly to collect contents at bottom of vial before opening.
Analysis Note
Purity: ≥ 98% confirmed by HNMR, LC-MS and HPLC and elemental analysis
Molar Mass: 625.65 g/mol
Molar Mass: 625.65 g/mol
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Este artículo | |||
|---|---|---|---|
| technique(s) cell based assay: suitable | technique(s) cell based assay: suitable | technique(s) cell based assay: suitable | technique(s) cell based assay: suitable |
| storage temp. -10 to -25°C | storage temp. -10 to -25°C | storage temp. -10 to -25°C | storage temp. - |
| assay ≥98% (H-NMR), ≥98% (LC/MS), ≥98% (HPLC), ≥98% (elemental analysis) | assay ≥98% (H-NMR), ≥98% (HPLC), ≥98% (LC/MS), ≥98% (elemental analysis) | assay ≥98% (H-NMR), ≥98% (HPLC), ≥98% (LC/MS), ≥98% (elemental analysis) | assay ≥98% (H-NMR), ≥98% (HPLC), ≥98% (LC/MS), ≥98% (elemental analysis) |
| shipped in ambient | shipped in ambient | shipped in ambient | shipped in - |
| detection method fluorometric | detection method fluorometric | detection method fluorometric | detection method fluorometric |
Clase de almacenamiento
11 - Combustible Solids
wgk
WGK 3
flash_point_f
Not applicable
flash_point_c
Not applicable
Certificados de análisis (COA)
Busque Certificados de análisis (COA) introduciendo el número de lote del producto. Los números de lote se encuentran en la etiqueta del producto después de las palabras «Lot» o «Batch»
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Contenido relacionado
Peter J Siska et al.
Journal of immunological methods, 438, 51-58 (2016-09-07)
T and B lymphocytes undergo metabolic re-programming upon activation that is essential to allow bioenergetics, cell survival, and intermediates for cell proliferation and function. To support changes in the activity of signaling pathways and to provide sufficient and necessary intracellular
Número de artículo de comercio global
| SKU | GTIN |
|---|---|
| SCT047 | 04054839532412 |
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