AB5705
Anti-Calcitonin Gene Related Protein Antibody
Chemicon®, from chicken
Sinónimos:
CGRP
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About This Item
UNSPSC Code:
12352203
eCl@ss:
32160702
NACRES:
NA.41
Productos recomendados
biological source
chicken
Quality Level
antibody form
purified immunoglobulin
antibody product type
primary antibodies
clone
polyclonal
species reactivity
rat
manufacturer/tradename
Chemicon®
technique(s)
immunohistochemistry: suitable
NCBI accession no.
UniProt accession no.
shipped in
dry ice
target post-translational modification
unmodified
Gene Information
human ... CALCA(796)
Specificity
CGRP (Calcitonin Gene Related Peptide).
Immunogen
A 14 amino acid synthetic peptide from rat CGRP I (processed active peptide). The immunogen sequence is identical at 13 of 14 residues between rat/mouse CGRP I and CGRP II. {KDNFVPTNVGSEAF-NH2}
Application
Immunohistochemistry: 1:500 See Protocol below.
Optimal working dilutions must be determined by the end user.
AB5705 has been used successfully on perfusion fixed tissue. Perfusion: 1) Ca2+-free Tyrode′s solution followed by 2) a paraformaldehyde-picric acid fixative and 3) 10% sucrose in PBS as a cryo-protectant. Tissues were rapidly dissected out and stored overnight in 0.1 M phosphate buffer (pH 7.4) containing 10% sucrose.Slide-mounted tissue sections were incubated with blocking buffer for 1 hour at room temperature. Primary antibody was diluted in blocking buffer to the appropriate working dilution. Blocking buffer was removed and the slides were then incubated at 2-8°C for 18-24 hours with AB5705. After rinsing in PBS 3 times sections were incubated for 60 minutes at room temperature with Cy3-conjugated secondary antibodies. After mounting in a mixture of PBS and glycerol (1:3) containing 0.1% p-phenylenediamine, sections were examined with a Nikon Microphot-SA epifluorescence microscope.
Optimal working dilutions must be determined by the end user.
AB5705 has been used successfully on perfusion fixed tissue. Perfusion: 1) Ca2+-free Tyrode′s solution followed by 2) a paraformaldehyde-picric acid fixative and 3) 10% sucrose in PBS as a cryo-protectant. Tissues were rapidly dissected out and stored overnight in 0.1 M phosphate buffer (pH 7.4) containing 10% sucrose.Slide-mounted tissue sections were incubated with blocking buffer for 1 hour at room temperature. Primary antibody was diluted in blocking buffer to the appropriate working dilution. Blocking buffer was removed and the slides were then incubated at 2-8°C for 18-24 hours with AB5705. After rinsing in PBS 3 times sections were incubated for 60 minutes at room temperature with Cy3-conjugated secondary antibodies. After mounting in a mixture of PBS and glycerol (1:3) containing 0.1% p-phenylenediamine, sections were examined with a Nikon Microphot-SA epifluorescence microscope.
Research Category
Neuroscience
Neuroscience
Research Sub Category
CNS Control of Metabolism
Hormones & Receptors
CNS Control of Metabolism
Hormones & Receptors
This Anti-Calcitonin Gene Related Protein Antibody is validated for use in IH for the detection of Calcitonin Gene Related Protein.
Physical form
Format: Purified
Purified chicken IgY. Liquid containing 0.05% sodium azide.
Storage and Stability
Maintain at -20°C in undiluted aliquots for up to 6 months after date of receipt. Avoid repeated freeze/thaw cycles.
Other Notes
Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.
Legal Information
CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Storage Class
10 - Combustible liquids
wgk_germany
WGK 1
Certificados de análisis (COA)
Busque Certificados de análisis (COA) introduciendo el número de lote del producto. Los números de lote se encuentran en la etiqueta del producto después de las palabras «Lot» o «Batch»
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