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492016

NP-40 Alternative

Sinónimos:

NP-40 Alternative - CAS 9016-45-9 - Calbiochem, Nonylphenyl Polyethylene Glycol

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Tamaño de envaseSKUDisponibilidadPrecio
100 mL
Comprobar disponibilidad del carrito
$95.10
500 mL
Comprobar disponibilidad del carrito
$278.00
1000 mL
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$463.00

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Número CAS:
UNSPSC Code:
12161902
NACRES:
NA.25
MDL number:

$95.10


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description

RTECS - MD0905000

Quality Level

form

liquid

manufacturer/tradename

Calbiochem®

storage condition

OK to freeze, desiccated

color

clear colorless

CMC

50 - 300 μM

solubility

water: 25 mg/mL, aqueous buffer: soluble

density

1.06 g/mL

application(s)

(Isolating membrane proteins)

shipped in

ambient

storage temp.

15-25°C

SMILES string

O(CCOc1ccc(cc1)CCCCCCCCC)CCO

InChI

1S/C19H32O3/c1-2-3-4-5-6-7-8-9-18-10-12-19(13-11-18)22-17-16-21-15-14-20/h10-13,20H,2-9,14-17H2,1H3

InChI key

BLXVTZPGEOGTGG-UHFFFAOYSA-N

General description

A non-ionic surfactant used in the isolation and purification of functional membrane protein complexes and in two-dimensional (2D) gel electrophoresis. Similar in usage to NP-40 and TRITON X-100 Detergent(Cat. No. 648462).

Application

NP-40 Alternative has been used:
  • as a component of the lysis buffer for solubilizing proteins from transfected HEK293 cells.
  • as a component of the wash buffer for efficient solubilization of biotinylated plasma proteins in plasma protein profiling and chromatin immunoprecipitation (ChIP).
  • for the purification of doublet microtubules from cilia for single particle cryo-electron microscopy (cryo-EM).

Other Notes

Ripoche, P., et al. 1982. Biochim. Biophys. Acta693, 497.

Legal Information

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany
Triton is a trademark of The Dow Chemical Company or an affiliated company of Dow

Disclaimer

Toxicity: Irritant (B)

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Este artículo
49201856741X45
form

liquid

form

solution

form

liquid

form

-

application(s)

(Isolating membrane proteins)

application(s)

(Isolating membrane proteins)

application(s)

-

application(s)

-

storage temp.

15-25°C

storage temp.

2-8°C

storage temp.

-

storage temp.

-

density

1.06 g/mL

density

-

density

1.06 g/mL at 20 °C

density

-

CMC

50 - 300 μM

CMC

50 - 300 μM

CMC

0.08 mM (20-25°C)

CMC

-

solubility

water: 25 mg/mL, aqueous buffer: soluble

solubility

water: soluble

solubility

-

solubility

-


pictograms

Exclamation markEnvironment

Clase de almacenamiento

11 - Combustible Solids

flash_point_f

503.6 °F - Pensky-Martens closed cup - (own results)

flash_point_c

262 °C - Pensky-Martens closed cup - (own results)

signalword

Danger

Hazard Classifications

Aquatic Acute 1 - Aquatic Chronic 1 - ED ENV 1 - Eye Irrit. 2 - Skin Irrit. 2



Certificados de análisis (COA)

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Journal of biological methods, 2(3), e23-e23 (2015-10-13)
Over the past two decades, researchers have struggled to efficiently express foreign DNA in primary macrophages, impeding research progress. The applications of lipofection, electroporation, microinjection, and viral-mediated transfer typically result in disruptions in macrophage differentiation and function, low expression levels
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Mutations in the channel protein PKD2 cause autosomal dominant polycystic kidney disease, but the function of PKD2 in cilia remains unclear. Here, we show that PKD2 targets and anchors mastigonemes, filamentous polymers of the glycoprotein MST1, to the extracellular surface



Número de artículo de comercio global

SKUGTIN
492016-100ML07790788050832
492016-1000ML04055977273489
492016-500ML04055977273496
492016-4L04061839404465

Questions

1–2 of 2 Questions  
  1. How can I determine the shelf life / expiration / retest date of this product?

    1 answer
    1. If this product has an expiration or retest date, it will be shown on the Certificate of Analysis (COA, CofA). If there is no retest or expiration date listed on the product's COA, we do not have suitable stability data to determine a shelf life. For these products, the only date on the COA will be the release date; a retest, expiration, or use-by-date will not be displayed.
      For all products, we recommend handling per defined conditions as printed in our product literature and website product descriptions. We recommend that products should be routinely inspected by customers to ensure they perform as expected.
      For products without retest or expiration dates, our standard warranty of 1 year from the date of shipment is applicable.
      For more information, please refer to the Product Dating Information document: https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/marketing/global/documents/418/501/product-dating-information-06-25-mk.pdf

      Helpful?

  2. How is shipping temperature determined? And how is it related to the product storage temperature?

    1 answer
    1. Products may be shipped at a different temperature than the recommended long-term storage temperature. If the product quality is sensitive to short-term exposure to conditions other than the recommended long-term storage, it will be shipped on wet or dry-ice. If the product quality is NOT affected by short-term exposure to conditions other than the recommended long-term storage, it will be shipped at ambient temperature. As shipping routes are configured for minimum transit times, shipping at ambient temperature helps control shipping costs for our customers. For more information, please refer to the Storage and Transport Conditions document: https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/marketing/global/documents/316/622/storage-transport-conditions-mk.pdf

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