04-858
Anti-Histone H4 Antibody, pan, rabbit monoclonal
culture supernatant, clone 62-141-13, Upstate®
Sinónimos:
H4, Histone H4
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| Tamaño de envase | SKU | Disponibilidad | Precio |
|---|---|---|---|
| 100 μL | Comprobar disponibilidad del carrito | $624.00 |
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UNSPSC Code:
12352203
NACRES:
NA.41
eCl@ss:
32160702
Conjugate:
unconjugated
Clone:
62-141-13, monoclonal
Application:
ChIP, WB, multiplexing
Citations:
41
$624.00
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Permítanos ayudarlebiological source
rabbit
Quality Level
conjugate
unconjugated
antibody form
culture supernatant
antibody product type
primary antibodies
clone
62-141-13, monoclonal
species reactivity
Saccharomyces cerevisiae, human, vertebrates, chicken
manufacturer/tradename
Upstate®
technique(s)
ChIP: suitable, multiplexing: suitable, western blot: suitable
isotype
IgG
NCBI accession no.
UniProt accession no.
shipped in
dry ice
target post-translational modification
unmodified
Gene Information
human ... H4C1(8359)
General description
10 kDa
This antibody recognizes histone H4 independant of any post-translational modifications.
Immunogen
Epitope: a.a. 25-28
Synthetic peptide corresponding to amino acids 17-28 of Histone H4. Epitope has been mapped to residues 25-28
Application
Anti-Histone H4 Antibody, pan is a Rabbit Monoclonal Antibody for detection of Histone H4 also known as Histone H4 & has been validated in ChIP, WB, Mplex.
Immunoblot Analysis:
A 1:100,000 dilution of a previous lot detected unmodified recombinant Histone H4 protein (Catalog # 14-412) and Histone H4 in acid extracts from both untreated HeLa cells and cells treated with sodium butyrate (hyper-acetylated) or colcemid (hyper-phosphorylated) (Figure B).
Chromatin Immunoprecipitation:
An independent laboratory used a previous lot of this antibody in chromatin immunoprecipitation (Issac, C., 2006).
Multiplexing:
Serial dilutions of a previous lot were incubated with histone peptides containing various unmodified peptides spanning the aminoterminus of Histone H4 conjugated to Luminex® microspheres. The antibody recognized both modified and unmodified peptides.
A 1:100,000 dilution of a previous lot detected unmodified recombinant Histone H4 protein (Catalog # 14-412) and Histone H4 in acid extracts from both untreated HeLa cells and cells treated with sodium butyrate (hyper-acetylated) or colcemid (hyper-phosphorylated) (Figure B).
Chromatin Immunoprecipitation:
An independent laboratory used a previous lot of this antibody in chromatin immunoprecipitation (Issac, C., 2006).
Multiplexing:
Serial dilutions of a previous lot were incubated with histone peptides containing various unmodified peptides spanning the aminoterminus of Histone H4 conjugated to Luminex® microspheres. The antibody recognized both modified and unmodified peptides.
Research Category
Epigenetics & Nuclear Function
Epigenetics & Nuclear Function
Research Sub Category
Histones
Histones
Biochem/physiol Actions
Recognizes Histone H4.
Physical form
Cultured supernantant in 0.05% sodium azide.
Preparation Note
Stable for 1 year at -20°C from date of receipt.
For maximum recovery of product, centrifuge the vial prior to removing the cap. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
For maximum recovery of product, centrifuge the vial prior to removing the cap. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
Analysis Note
Control
Recombinant Histone H4 (Catalog # 14-412) or HeLa cell acid extract.
Recombinant Histone H4 (Catalog # 14-412) or HeLa cell acid extract.
Routinely evaluated by immunoblot using HeLa cell acid extract.
Other Notes
Replaces: 05-858
Legal Information
Luminex is a registered trademark of Luminex Corporation
UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Este artículo | |||
|---|---|---|---|
| clone 62-141-13, monoclonal | clone 62-141-13, monoclonal | clone 62-10C-2, monoclonal | clone polyclonal |
| antibody form culture supernatant | antibody form purified immunoglobulin | antibody form culture supernatant | antibody form serum |
| species reactivity Saccharomyces cerevisiae, human, vertebrates, chicken | species reactivity chicken, Saccharomyces cerevisiae, vertebrates, yeast, human | species reactivity human | species reactivity human, Saccharomyces cerevisiae |
| biological source rabbit | biological source rabbit | biological source rabbit | biological source rabbit |
| Gene Information human ... H4C1(8359) | Gene Information Saccharomyces cerevisiae ... Hhf1(852294) | Gene Information human ... H4C1(8359) | Gene Information human ... H4C1(8359) |
| conjugate unconjugated | conjugate unconjugated | conjugate unconjugated | conjugate - |
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Encuentre la documentación para los productos que ha comprado recientemente en la Biblioteca de documentos.
Multiple forms of H4 histone mRNA in human cells.
Lichtler, A C, et al.
Proceedings of the National Academy of Sciences of the USA, 77, 1942-1946 (1980)
Deepika Jaiswal et al.
G3 (Bethesda, Md.), 7(12), 3971-3982 (2017-10-27)
The conserved yeast histone methyltransferase Set1 targets H3 lysine 4 (H3K4) for mono, di, and trimethylation and is linked to active transcription due to the euchromatic distribution of these methyl marks and the recruitment of Set1 during transcription. However, loss
Anne de Bruyn Kops et al.
Molecular biology of the cell, 29(21), 2578-2590 (2018-08-23)
Correlation between transcriptional regulation and positioning of genes at the nuclear envelope is well established in eukaryotes, but the mechanisms involved are not well understood. We show that brr6-1, a mutant of the essential yeast envelope transmembrane protein Brr6p, impairs
Número de artículo de comercio global
| SKU | GTIN |
|---|---|
| 04-858 | 04053252337130 |