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SEQR

SeqPlex RNA Amplification Kit

For use with high throughput sequencing technologies

Synonyme(s) :

WGA kit, Whole transcriptome amplification

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A propos de cet article

NACRES:
NA.55
UNSPSC Code:
41121800

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technique(s)

whole genome amplification: suitable

Quality Level

200

dilution

(WTA)

input

purified RNA

shipped in

wet ice

storage temp.

−20°C

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Cet article
SEQXEWTA2WGA2
SeqPlex RNA Amplification Kit For use with high throughput sequencing technologies

Sigma-Aldrich

SEQR

SeqPlex RNA Amplification Kit

SeqPlex DNA Amplification Kit For use with high throughput sequencing technologies, Whole Genome Amplification kit designed to facilitate Next Gen Sequencing.

Sigma-Aldrich

SEQXE

SeqPlex DNA Amplification Kit

Complete Whole Transcriptome Amplification Kit DNA polymerase included, Complete Kit with optimized enzyme to amplify total RNA in <4 hours, no 3′ bias

Sigma-Aldrich

WTA2

Complete Whole Transcriptome Amplification Kit

GenomePlex® Complete Whole Genome Amplification (WGA) Kit Optimized kit with enzyme for amplifying a variety of DNA including FFPE tissue

Sigma-Aldrich

WGA2

GenomePlex® Complete Whole Genome Amplification (WGA) Kit

technique(s)

whole genome amplification: suitable

technique(s)

whole genome amplification: suitable

technique(s)

whole genome amplification: suitable

technique(s)

whole genome amplification: suitable

shipped in

wet ice

shipped in

wet ice

shipped in

wet ice

shipped in

wet ice

storage temp.

−20°C

storage temp.

−20°C

storage temp.

−20°C

storage temp.

−20°C

Quality Level

200

Quality Level

200

Quality Level

200

Quality Level

200

General description

The SeqPlex™ RNA Amplification Kit for whole transcriptome amplification (WTA) is designed to facilitate next-generation sequencing (NGS) from small quantities or from degraded/highly fragmented RNA (e.g. RNA from formalin-fixed paraffin-embedded (FFPE) tissue samples). The SeqPlex kit allows the user to pre-amplify these and other small quantity/highly fragmented RNA samples for input into a NGS workflow. It also facilitates the amplification of non-polyA tailed RNA isolated from tissue, cultured cells, formalin-fixed samples, or serum while maintaining patterns of differential expression found in the unamplified sample.

Application

SeqPlex™ RNA Amplification Kit has been used to amplify poly(A)-selected RNA.[1][2]

Features and Benefits

  • Low quantities of total RNA random priming technology amplifies fragmented or intact RNA from all sources including FFPE and RIP.
  • Semi-degenerate library primer design for more complete transcriptome coverage and efficient priming.
  • No need to fragment DNA before sequencing.
  • Amplifies ds-cDNA in 8 hours or less.
  • Compatible with all next generation sequencing platforms except Pacific Bioscience.

Other Notes

SEQR-500RXN is manufactured on-demand. Contact us for more information.

Legal Information

SeqPlex is a trademark of Sigma-Aldrich Co. LLC

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TransPlex® Whole Transcriptome Amplification Kit, DNA polymerase separate.

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Classe de stockage

11 - Combustible Solids

flash_point_f

Not applicable

flash_point_c

Not applicable

wgk

WGK 3

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Certificats d'analyse (COA)

Lot/Batch Number
0000505089
0000505090
0000505090
0000505089
0000401679

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Consulter la Bibliothèque de documents

Isaac Engel et al.
Nature immunology, 17(6), 728-739 (2016-04-19)
Natural killer T cells (NKT cells) have stimulatory or inhibitory effects on the immune response that can be attributed in part to the existence of functional subsets of NKT cells. These subsets have been characterized only on the basis of
DNA Sequencing Research Group (DSRG): Evaluation of RNA Amplification Kits at Subnanogram Input Amounts of Total RNA for RNA-Seq
Nicolet C, et al.
Journal of biomolecular techniques : JBT, 24, S70-S70 (2013)
Meritxell Jodar et al.
Human reproduction update, 19(6), 604-624 (2013-07-17)
BACKGROUND Spermatozoa are highly differentiated, transcriptionally inert cells characterized by a compact nucleus with minimal cytoplasm. Nevertheless they contain a suite of unique RNAs that are delivered to oocyte upon fertilization. They are likely integrated as part of many different
Giuliana P Mognol et al.
Proceedings of the National Academy of Sciences of the United States of America, 114(13), E2776-E2785 (2017-03-12)
T-cell exhaustion is a progressive loss of effector function and memory potential due to persistent antigen exposure, which occurs in chronic viral infections and cancer. Here we investigate the relation between gene expression and chromatin accessibility in CD8+ tumor-infiltrating lymphocytes
Kathryn D Tuttle et al.
Nature communications, 9(1), 2650-2650 (2018-07-10)
During development in the thymus, invariant natural killer T (iNKT) cells commit to one of three major functionally different subsets, iNKT1, iNKT2, and iNKT17. Here, we show that T cell antigen receptor (TCR) signal strength governs the development of iNKT
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Protocoles

SEQR - SeqPlex RNA Amplification Kit Protocol

The SeqPlex RNA Amplification kit provides a method for amplification of total RNA or isolated mRNA prior to entry into the workflows of the commonly used deep sequencing platforms.

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