SEQR
SeqPlex RNA Amplification Kit
For use with high throughput sequencing technologies
Synonyme(s) :
WGA kit, Whole transcriptome amplification
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| À vous/Référence | Disponibilité | Prix |
|---|---|---|
10 reactions | Vérifier la disponibilité des articles du panier | 1 030.00 CHF |
50 reactions | Vérifier la disponibilité des articles du panier | 4 670.00 CHF |
500 reactions | Vérifier la disponibilité des articles du panier | 23 460.00 CHF |
A propos de cet article
NACRES:
NA.55
UNSPSC Code:
41121800
1 030.00 CHF
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technique(s)
whole genome amplification: suitable
dilution
(WTA)
input
purified RNA
shipped in
wet ice
storage temp.
−20°C
General description
The SeqPlex™ RNA Amplification Kit for whole transcriptome amplification (WTA) is designed to facilitate next-generation sequencing (NGS) from small quantities or from degraded/highly fragmented RNA (e.g. RNA from formalin-fixed paraffin-embedded (FFPE) tissue samples). The SeqPlex kit allows the user to pre-amplify these and other small quantity/highly fragmented RNA samples for input into a NGS workflow. It also facilitates the amplification of non-polyA tailed RNA isolated from tissue, cultured cells, formalin-fixed samples, or serum while maintaining patterns of differential expression found in the unamplified sample.
Features and Benefits
- Low quantities of total RNA random priming technology amplifies fragmented or intact RNA from all sources including FFPE and RIP.
- Semi-degenerate library primer design for more complete transcriptome coverage and efficient priming.
- No need to fragment DNA before sequencing.
- Amplifies ds-cDNA in 8 hours or less.
- Compatible with all next generation sequencing platforms except Pacific Bioscience.
Other Notes
SEQR-500RXN is manufactured on-demand. Contact us for more information.
Legal Information
SeqPlex is a trademark of Sigma-Aldrich Co. LLC
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Cet article | |||
|---|---|---|---|
| technique(s) whole genome amplification: suitable | technique(s) whole genome amplification: suitable | technique(s) whole genome amplification: suitable | technique(s) whole genome amplification: suitable |
| Quality Level 200 | Quality Level 200 | Quality Level 200 | Quality Level 200 |
| dilution (WTA) | dilution (WGA) | dilution (WTA) | dilution (WTA) |
| shipped in wet ice | shipped in wet ice | shipped in wet ice | shipped in wet ice |
| input purified RNA | input purified DNA | input purified RNA | input purified DNA |
| storage temp. −20°C | storage temp. −20°C | storage temp. −20°C | storage temp. −20°C |
Classe de stockage
11 - Combustible Solids
flash_point_f
Not applicable
flash_point_c
Not applicable
wgk
WGK 3
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